Challenging mass culture of mesenchymal stem cells: Soluble microcarriers

― Made from raw materials that conform to the Standards for Biological Raw Materials －

Removal of microcarriers

When mass culturing with microcarriers made of resin, cells must be harvested by filtration after culture. However, removing small debris that is generated during culture is difficult.
Atelocollagen Microspheres are produced only from atelocollagen, which can be completely removed (dissolved).

When a crude collagenase preparation (200 units/mg) containing various common hydrolytic enzymes used for cell dispersion was added to the microspheres to a final concentration of 0.1% and incubated at 37°C, the microspheres were completely dissolved after 60 min* (based on in-house data).

*Different types of collagenase are available on the market, and collagenase activity varies greatly. Therefore , the dissolution time varies depending on the collagenase used and its concentration.

Potential elimination of the need for microcarrier removal

The following paper showed that microspheres were dissolved by synovial fluid from patients with knee osteoarthritis, indicating the potential of microspheres to be administered directly as a transplant carrier after mass cultivation.

A novel cell source for therapy of knee osteoarthritis using atelocollagen microsphere-adhered adipose-derived stem cells: Impact of synovial fluid exposure on cell activity.

Sakamoto T, et al. Regen Ther. 2024 Apr 23;27:408-418. PMID: 38694445.

Adipose-derived mesenchymal stem cells were seeded onto the microspheres and cultured in a rotational system. In addition, the degradation of the microspheres by synovial fluid was evaluated.

Soluble microcarriers made from atelocollagen

Atelocollagen Ｍicrospheres　15mL/bottle

Seminar information

We plan to hold the following seminar in August 2025. We hope you will join us to take an opportunity to have the recent information on regenerative medicine, genome editing, and organoids.