Collagen for Cell Culture and RNAi Techonology
Atelocollagen for researchー Connecting basic research to clinical applications ー
- Poster presentation at 5th Tissue Engineering and Regenerative Medicine International Society -World Congress (TERMIS-WC)
- Poster presentation at 17th Congress of the Japanese Society for Regenerative Medicine (JSRM)
- Poster presentation at Consortium of Biosciences 2017 (ConBio2017)
- The 138th Annual Meeting of the Pharmaceutical Society of Japan (AMPSJ)
- The 17th Congress of the Japanese Society for Regenerative Medicine (17JSRM)
- The 42nd Annual Meeting of the Japanese Society for Investigative Dermatology
- Use of collagen microspheres for sustained release of growth factors and high-density cell culture
- Atelocollagen gel for transplantation of 3D cultured skin
- Atelocollagen permeable membrane for transplantation of cell sheets
11.09.2018Open innovation for advance in Life science research
Open innovation has been increasingly h...
10.07.2018Molecular crowding affects induction of stem cell differentiation
We guess for many people the term molec...
29.05.2018Does collagen coating influence experimental results?
Collagen is widely used as a coating fo...
What is Atelocollagen?
Collagen is an extracellular matrix found in the dermis, ligaments, bones, etc., and accounts for approximately 30% of the total protein in the human body. The most abundant type of collagen is type I collagen, which has a molecular weight of approximately 300 kDa and comprises three polypeptides. The molecular structure of collagen is a right-handed triple helix region and telopeptide (non-helical) regions at the N-terminal and C-terminal of the molecule. These telopeptide regions are composed of two α1 chains and one α2 chain. The triple helix region is conserved among species and shows low immunogenicity, while the telopeptide regions exhibit high immunogenicity. Removal of the telopeptide regions by protease treatment produces atelocollagen, which retains the same properties as collagen. We have developed atelocollagen-based medical devices.
Unwinding of the triple helix of collagen and atelocollagen by heat degeneration produces gelatine. Gelatine is a random coil single polypeptide and has high immunogenicity.Peptides obtained by hydrolysis with strong acids, strong alkalis, or by enzymatic treatment are called hydrolysed collagen. Gelatine and hydrolysed collagen have totally different properties from collagen due to their structural differences compared to collagen.