FibColl^® accelerated stratum corneum maturation in an epidermal model

FibColl^® Atelocollagen Inserts (FibColl^®) and polyethylene terephthalate (PET) cell culture inserts were used to culture keratinocytes at the air–liquid interface for 14 days under the same culture conditions. Frozen sections were then evaluated through hematoxylin and eosin staining. A well-developed stratum corneum was observed on FibColl^® as early as day 7 of culture, and further stratification was observed on day 14.

FibColl^® allowed for the expression of barrier function markers and improved transepithelial electrical resistance (TEER)

First, keratinocytes were cultured at the air–liquid interface for 7 days on FibColl^® and PET inserts. Frozen sections were then examined through fluorescent immunostaining. Tight junctions formed by CLDN1 and the clear expression of OCLN (Occludin) were observed in FibColl^®. In addition, transepithelial electrical resistance (TEER) values were significantly higher in the FibColl^® group than in the PET inserts group (based on in-house data).

Insert containing 100% atelocollagen membrane

Fibcoll^® Atelocollagen Inserts 24 (24 pieces/bag)

Unlike other collagen-coated plastic membranes, the pores in FibColl^® remain unclogged, and its atelocollagen fiber structure facilitates cell culture in an environment that closely mimics in vivo conditions.

Long-term culture of the dermal layer inhibited contraction in a full-thickness skin model

When creating a full-thickness skin model using native collagen (NC) and atelocollagen (AC), we cultured a dermal layer comprising collagen gel with fibroblasts for 14 days (25 days in total, including epidermal layer formation) instead of the usual 7 days (18 days, including epidermal layer formation). In-house data revealed that the shrinkage could be suppressed by culturing the dermal layer for 14 days (25 days, including surface layer formation) instead of the usual 7 days (18 days, including epidermal layer formation).

Application of a contraction-suppressed full-thickness skin model to a psoriasis-like inflammation model

Furthermore, psoriasis-like inflammation was induced in a contraction-suppressed full-thickness skin model (CTR) in the psoriasis group (Ps) by cytokines (IL-1α, IL-6, TNF-α, IL-22, and IL-17A) that are characteristic of psoriasis. The nuclei remained in the stratum corneum, presumably because of incomplete keratinization, and expression of psoriasis-specific genes and changes in protein expression were observed in the group (based on in-house data).

Ready-to-use premixed atelocollagen solution

3D Ready Atelocollagen (12 mL/bottle)

This is an atelocollagen solution for three-dimensional (3D) cultures with the intention of making stable 3D culture accessible to everyone. Since DMEM is premixed with high-purity atelocollagen, it can be used immediately by simply mixing it with cells and warming it up.

Exhibition information

We plan to participate in the upcoming cosmetic exhibition in May 2025. Please visit us at the venue to explore our atelocollagen products, which are suitable for creating epidermal and full-thickness skin models.