FibColl® for Producing matured epidermal models | Koken Co. Ltd.

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FibColl® Atelocollagen Inserts 24

Outline

FibColl® Atelocollagen Insert 24 is a novel cell culture insert that uses a membrane entirely made from atelocollagen. Unlike other collagen-coated plastic membranes, FibColl® pores are not clogged, and the fiber structure of atelocollagen enables cell culture in an in vivo mimicking environment. The microporous structure between atelocollagen fibrils allows the permeation of molecules even over 600 kDa, making it suitable for barrier function assessment and co-culture models.

Applications

  • Culture-on-FibColl

    Culture on the membrane

  • Air-liquid interface culture on FibColl

    Air-liquid interface culture

  • Co-culture-with-FibColl

    Co-culture

  • Cell-sheet-transplantation-with-FibColl

    Cell sheet transplantation

Features

  • Membranes made from atelocollagen are permeable to molecules with molecular weight even higher than 600 kDa.
  • A barrier function assessment model can be developed by tight junction formation of epithelial cells.
  • FibColl® is an easy-to-use hanging cell culture insert considering medium exchange and membrane recovery after culture.
  • The membrane is biodegradable and therefore it can also be used for cell sheet transplantation.

Types of collagen

Bovine hide derived atelocollagen

  • Fibril structure of FibColl® (SEM image)

  • Lung cancer cells cultured on FibColl® for 2 days (optical phase-contrast microscopy image)

  • Formation of tight junctions between renal tubular epithelial cells cultured using FibColl® for 2 days (Red: ZO-1, blue: nucleus, fluorescence microscope image).）

Cat. No. Product Packaging Price
FAI-24 FibColl® Atelocollagen Inserts 24 24 pieces/bag Visit distributor’s
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Storage: Room temperature  For research use only.

Click here for frequently asked questions about FibColl® Atelocollagen Inserts

Use case

  • Use Case 1
  • Use Case 2
  • Use Case 3

  • Accelerated maturation of epidermal model using FibColl®

    Normal human epidermal keratinocytes (1.8×10⁵ cells) were seeded on the membrane of FibColl® or polyethylene terephthalate cell culture inserts (PET), and cultured submerged for 3 days, followed by air-liquid interface culture for 14 days. Frozen sections of each epidermal model were then observed using HE staining. As a result, a mature stratum corneum was formed on FibColl® as early as day 7 of culture, and further layering of the stratum corneum was confirmed on day 14 (In-house data).

    When the expression of each epidermal marker was observed by immunofluorescence staining, maximum expression of CK14, a basal layer marker, was observed near the basal layer with FibColl®.In addition, the staining distinction between CK14 and the spinous layer marker CK10 was clearer than with PET. Furthermore, expression of FLG, a granular layer marker, near the granular layer was prominent (In-house data).

    In FibColl® culture, clear expression of CLDN1 and OCLN, components of tight junctions, was confirmed by immunostaining. In addition, TEER measurement showed that FibColl® had a significantly higher TEER value than PET (In-house data).

  • Evaluation of substance permeability and barrier function using FibColl®

    ①After adding a solution of the evaluation molecule onto the FibColl® membrane, the molecules that migrated under the membrane were quantified. Proteins over 600 kDa also permeated.
    ② Canine renal tubular epithelial cells (MDCK cells) were cultured on FibColl® for 14 days, and the formation of tight junctions was confirmed by immunofluorescent staining of ZO-1.
    ③ Transepithelial electrical resistance (TEER) measurements showed electrical resistance.

    (In-house data)

  • Application of FibColl® in a psoriasis-like epidermal inflammation model

    In an epidermal model cultured for 7 days at the air-liquid interface on FibColl®, cytokines were added twice at 24-hour intervals after medium exchange to induce inflammation. Expression of several psoriasis-related genes was found to be upregulated or downregulated, indicating the utility of FibColl® in constructing psoriasis models. Sufficient RNA was extractable from FibColl®(24 well plate size) for qPCR analysis (In-house data).

References

  • Culture on the membrane
  • Co-culture
  • Cell sheet transplantation