Outline

FibColl^® Atelocollagen Insert 24 is a novel cell culture insert that uses a membrane entirely made from atelocollagen. Unlike other collagen-coated plastic membranes, FibColl^® pores are not clogged, and the fiber structure of atelocollagen enables cell culture in an in vivo mimicking environment. The microporous structure between atelocollagen fibrils allows the permeation of molecules even over 600 kDa, making it suitable for barrier function assessment and co-culture models.

Applications

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  Culture on the membrane

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  Air-liquid interface culture

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  Co-culture

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  Cell sheet transplantation

Features

• Membranes made from atelocollagen are permeable to molecules with molecular weight even higher than 600 kDa.
• A barrier function assessment model can be developed by tight junction formation of epithelial cells.
• FibColl^® is an easy-to-use hanging cell culture insert considering medium exchange and membrane recovery after culture.
• The membrane is biodegradable and therefore it can also be used for cell sheet transplantation.

Types of collagen

Bovine hide derived atelocollagen

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  Fibril structure of FibColl^® (SEM image)

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  Lung cancer cells cultured on FibColl^® for 2 days (optical phase-contrast microscopy image)

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  Formation of tight junctions between renal tubular epithelial cells cultured using FibColl^® for 2 days (Red: ZO-1, blue: nucleus, fluorescence microscope image).）

Use case

References