3D Ready Atelocollagen for Organoid/3D culture | Koken Co., Ltd.

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3D Ready Atelocollagen

Outline

Stable three-dimensional culture (3D culture) can be performed easily without the need for neutralization or mixing with cell culture medium usually required to form atelocollagen gels. High-purity atelocollagen derived from bovine hide is premixed with DMEM, so it can be used immediately. Simply mix with the cells and warm it up. This product is especially recommended for those beginning the 3D culture without worrying about fibril formation during mixing the solutions or cell sedimentation during gel formation.

Applications

  • Collagen-gel-3D-culture

    3D culture

  • Culture-on-a-collagen-gel

    Culture on collagen gel

  • Collagen-gel-3D-co-culture

    Co-culture

Features

  • Ready-to-use atelocollagen solution for 3D culture.

  • Premixed with DMEM, low glucose or high glucose.

  • Easy operation: simply mix with the cells and warm to form a gel.

  • Reduces cell sedimentation during gel formation achieves a stable 3D culture.

Type of collagen

Bovine hide derived atelocollagen

Notice of temporary container change
Regarding 3D-LG01 and 3D-LG05,  we may temporarily change from 20 mL container to 30 mL container due to container shortage. In this case, 3D-LG05 will be individually wrapped 5 bottles instead of a set of 5 bottles in a box. There is no change in catalogue number, specifications, price, or ordering method due to temporary changes in the container.

Cat. No. Product Packaging Price
3D-LG01 3D Ready Atelocollagen DMEM-LG1（Low Glucose） 12 mL/bottle Visit distributor’s
web page
3D-LG05 3D Ready Atelocollagen DMEM-LG5（Low Glucose） 12 mL/bottle×5 bottles Visit distributor’s
web page
3D-HG01 3D Ready Atelocollagen DMEM-HG1（High Glucose） 12 mL/bottle Visit distributor’s
web page
3D-HG05 3D Ready Atelocollagen DMEM-HG5（High Glucose） 12mL/bottle×5 bottles Visit distributor’s
web page

Storage: -20℃  For research use only.

Click here for frequently asked questions about 3D ready atelocollagen

Use case

  • Use case 1
  • Use case 2
  • Use case 3
  • Use case 4
  • Use case 5

  • Are your cells sedimenting in your 3D culture?

    3D Ready Atelocollagen enables uniform 3D culture. Cell sedimentation was suppressed in in-gel culture of GFP-expressing HepG2 cells by using 3D Ready Atelocollagen. (in-house data)

  • Evaluation of cell spreading using various types of cells

    This is the result of creating a gel containing various cells stained with Hoechst 33342 using 3D atelocollagen and observing the cross section of the gel.
    Cell sedimentation to the bottom of the gel was suppressed, and cell dispersion could be observed near the top of the gel. This shows that uniform 3D culture of multiple cell types is possible.　(in-house data)

  • Evaluation of cell invasion from cancer cell spheroids

    3D Ready Atelocollagen is transparent enough for microscopic observation.
    After forming spheroids of various cells using a low-adhesion plate, 3D Ready Atelocollagen was added to the wells to perform in-gel culture (3D culture).
    As a result, cell infiltration was observed in all cell types after 2 days of culture.
    (Fujita M, et al. National Institutes for Quantum Science and Technology )

  • Atelocollagen as a defined alternative to soluble basement membrane extract!

    Comparison study of organoids cultured with basement membrane extract vs. atelocollagen concluded no major differences in morphology or ability to secrete water.
    Note: In-house data below obtained with ~0.5% atelocollagen.3D Ready Atelocollagen product is ~0.4 %

    Prior to induction with differentiation factors, no significant differences in marker gene expression were observed between atelocollagen cultured and basement membrane extract cultured organoids. In atelocollagen cultures, addition of differentiation factors significantly increased marker gene expression.
    Note: In-house data below obtained with ~0.5% atelocollagen.3D Ready Atelocollagen product is ~0.4 %

  • Functional, defined organoid culture at less cost!

    As expected, prolactin addition stimulated casein gene expression in mouse mammary gland organoids produced with atelocollagen, showing that 3D Ready AteloCell can be an effective, cost-efficient alternative to undefined basement membrane extract scaffolds that may also introduce ambiguity into experimental data interpretation.
    Note: In-house data below obtained with ~0.5% atelocollagen.3D Ready Atelocollagen product is ~0.4 %

References

  • 3D culture
  • Co-culture
  • Miscellaneous
    • This product is a new product that eliminates the labor of neutralizing Collagen acidic solutions and mixing a medium. You can see many reference papers on the product page of Collagen acid solutions.
    • Effects of Wnt10a on dentinogenesis of dental pulp cells in a 3-dimensional culture system using atelocollagen gel.
      Fujisawa H, Kato Y, Yokose S.
      Odontology. 2025 Jun 25. Epub ahead of print. PMID: 40560351.
    • Collagen Depletion by Pirfenidone Enhances Antitumor Effect of Oncolytic Adenovirus against Peritoneal Metastases of Gastric Cancer
      Okura, Tomohiro et al.
      Molecular Therapy Oncology, Volume 0, Issue 0, 201045
    • Protocol for RNA extraction from glioblastoma cell-line-derived spheroids embedded in atelocollagen gel guided by real-time imaging.
      Fujita M, Imadome K, Sato T, Hirakawa H, Kado J, Yamagiri A, Miura T, Kamimura S.
      STAR Protoc. 2025 May 24;6(2):103839. PMID: 40413748.
    • COL8A1 enhances the invasion/metastasis in MDA-MB-231 cells via the induction of IL1B and MMP1 expression.
      Sagara A, Miura S, Kobinata A, Naganawa R, Yaginuma S, Saito S, Saito R, Kominato H, Yumoto T, Sato F.
      Biochem Biophys Res Commun. 2023 Jan 29;642:145-153. PMID: 36577251.
    • Decreased levels of endocytic collagen receptor Endo180 in dermal fibroblasts lead to decreased production of type I collagen and increased expression of matrix metalloproteinase-1.
      Iwahashi H, Kawashima Y, Masaki H.
      Photodermatol Photoimmunol Photomed. 2022 Mar;38(2):150-157. PMID: 34469613.
    • Lotus germ extract rejuvenates aging fibroblasts via restoration of disrupted proteostasis by the induction of autophagy.
      Machihara K, Kageyama S, Oki S, Makino H, Sasaki M, Iwahashi H, Namba T.
      Aging (Albany NY). 2022 Sep 26;14(19):7662-7691. PMID: 36170016.
    • Metabolic characterization of aggressive breast cancer cells exhibiting invasive phenotype: impact of non-cytotoxic doses of 2-DG on diminishing invasiveness.
      Fujita M, Imadome K, Somasundaram V, Kawanishi M, Karasawa K, Wink DA.
      BMC Cancer. 2020 Sep 29;20(1):929. PMID: 32993545
    • This product is a new product that eliminates the labor of neutralizing Collagen acidic solutions and mixing a medium. You can see many reference papers on the product page of Collagen acid solutions.
    • This product is a new product that eliminates the labor of neutralizing Collagen acidic solutions and mixing a medium. You can see many reference papers on the product page of Collagen acid solutions.
    • Cell Morphology-Based Screening Identified Vitetrifolin D from Vitex Rotundifolia as an Inhibitor of Phorbol Ester–Induced Downregulation of E-Cadherin in HHUA Endometrial Cells,
      Yusuke Hanaki, Nichika Iwase, Yasunori Sugiyama, Sena Miyoshi, Ryo C. Yanagita,
      BPB Reports, 2023 6(3) 103-107.
    • Hypercholesterolemic Dysregulation of Calpain in Lymphatic Endothelial Cells Interferes With Regulatory T-Cell Stability and Trafficking.
      Miyazaki T, Taketomi Y, Higashi T, Ohtaki H, Takaki T, Ohnishi K, Hosonuma M, Kono N, Akasu R, Haraguchi S, Kim-Kaneyama JR, Otsu K, Arai H, Murakami M, Miyazaki A.
      Arterioscler Thromb Vasc Biol. 2023 Feb;43(2):e66-e82. PMID: 36519468.
    • 12-O-Tetradecanoylphorbol 13-acetate promotes proliferation and epithelial-mesenchymal transition in HHUA cells cultured on collagen type I gel: A feasible model to find new therapies for endometrial diseases.
      Hanaki Y, Miyoshi S, Sugiyama Y, Yanagita RC, Sato M.
      Biosci Biotechnol Biochem. 2022 Sep 23;86(10):1417-1422. PMID: 35973688.
    • Newly established patient-derived organoid model of intracranial meningioma.
      Yamazaki S, Ohka F, Hirano M, Shiraki Y, Motomura K, Tanahashi K, Tsujiuchi T, Motomura A, Aoki K, Shinjo K, Murofushi Y, Kitano Y, Maeda S, Kato A, Shimizu H, Yamaguchi J, Adilijiang A, Wakabayashi T, Saito R, Enomoto A, Kondo Y, Natsume A.
      Neuro Oncol. 2021 Nov 2;23(11):1936-1948. PMID: 34214169.