AteloGene® Systemic Use for in vivo transfection | KOKEN CO., LTD. | KOKEN CO., LTD.

Show product listProduct list

AteloGene® Systemic Use


This in vivo transfection kit is designed for delivering siRNA/miRNA into animal tissues, mouse in particular, to introduce the siRNA/miRNA into cells by systemic administrations.

Atelocollagen is positively charged and can thus electrostatically form complexes with nucleic acids when they are mixed together at appropriate concentrations and ratios. The complexes protect nucleic acids from being degraded by nucleases in in vivo.

What is Atelocollagen?



  • i.v. administration of nucleic acids

  • i.p. administration of nucleic acids



  • Prolonged in vivo RNAi effect because atelocollagen, the main component of AteloGene® products, form complexes with siRNA/miRNA.
  • Since AteloGene® products are made from atelocollagen, it is nontoxic.
  • AteloGene® Systemic Use does not form a gel and siRNA/miRNA are efficiently delivered throughout the entire body via the blood circulation following tail vein injection.

Kit contents

①AteloGene® Systemic Use prefilled syringe
  (Including losses during the preparation steps)
600 μL × 2 syringes
②10×siRNA buffer
    (It can be used for miRNA as well)
3 mL × 1 bottle
③Sterilized water 3 mL × 1 bottle
④2 mL microtube 2 tubes
⑤Disposable Syringe 2 tubes
⑥18G needle 4 needles
⑦26G needle 2 needles
⑧Instruction manual 1 leaflet


Devices and reagents required other than those in the kit

  • siRNA/miRNA (PAGE- or HPLC-purified grade is recommended.)
  • Container for preparation of siRNA/miRNA solution (sterilized, RNase-free)
  • Cooling device (crushed ice, cold block, etc.)
  • Tube rotator (that can tumble and agitate such as TAITEC RT-5, Bibby scientific SB3, etc.)
  • Pipetter and tips (sterilized, RNase-free)
  • High-speed refrigerated centrifuge
  • Anesthetic
Cat. No. Product Package Price
for Cosmo Bio customers

for REPROCELL customers

AteloGene® Systemic Use 1 kit
(10 administrations) *
Visit distributor’s
web page

*It can be used more than 10 administrations depending on target tissue because one administration is assumed to be 200μl.

Storage: 2-10°C  For research use only.
Expiration date: 5 years from manufacturing date printed on the box.

Click here for frequently asked questions about AteloGene® Systemic Use

Experimental example

Systemic siRNA administration to mouse metastatic tumour model with AteloGene® Systemic Use

After systemic administration of a luciferase siRNA (Luc siRNA) & AteloGene® mixture to metastatic tumors expressing luciferase, luciferase expression was markedly reduced when compared to the control.


  • Systemic administration in Cancer research
  • Systemic administration in other research
    • Treating metastatic prostate cancer with microRNA-145.
      Iscaife A, Reis ST, Morais DR, Viana NI, da Silva IA, Pimenta R, Bordini A, Dip N, Srougi M, Leite KRM.
      Apoptosis. 2018 Aug;23(7-8):388-395. PMID: 29858716
    • Combined anti-metastasis therapy of an siRNA- based medicine and ABT-263 in orthotopically xenografted prostate cancer model mice.
      TAKEI, Yoshifumi et al.
      Medical Research Archives, [S.l.], v. 6, n. 9, sep. 2018.
    • Histone deacetylase inhibitors inhibit metastasis by restoring a tumor suppressive microRNA-150 in advanced cutaneous T-cell lymphoma.
      Abe F, Kitadate A, Ikeda S, Yamashita J, Nakanishi H, Takahashi N, Asaka C, Teshima K5, Miyagaki T, Sugaya M, Tagawa H.
      Oncotarget. 2017 Jan 31;8(5):7572-7585. PMID: 27935859
    • miR-340 suppresses tumor growth and enhances chemosensitivity of colorectal cancer by targeting RLIP76.
      Zhang LL, Xie FJ, Tang CH, Xu WR, Ding XS, Liang J.
      Eur Rev Med Pharmacol Sci. 2017 Jun;21(12):2875-2886. PMID: 28682430
    • Efficient delivery of micro RNA to bone-metastatic prostate tumors by using aptamer-conjugated atelocollagen in vitro and in vivo.
      Hao Z, Fan W, Hao J, Wu X, Zeng GQ, Zhang LJ, Nie SF, Wang XD.
      Drug Deliv. 2016;23(3):874-81. PMID: 24892627
    • MiR675-5p Acts on HIF-1α to Sustain Hypoxic Responses: A New Therapeutic Strategy for Glioma.
      Lo Dico A, Costa V, Martelli C, Diceglie C, Rajata F, Rizzo A, Mancone C, Tripodi M, Ottobrini L, Alessandro R, Conigliaro A.
      Theranostics. 2016 May 8;6(8):1105-18. PMID: 27279905
    • Identification of Akt1 as a potent therapeutic target for oral squamous cell carcinoma.
      Nakashiro K, Tanaka H, Goda H, Iwamoto K, Tokuzen N, Hara S, Onodera J, Fujimoto I, Hino S, Hamakawa H.
      Int J Oncol. 2015 Oct;47(4):1273-81. PMID: 26315674
    • Atelocollagen-mediated intravenous siRNA delivery specific to tumor tissues orthotopically xenografted in prostates of nude mice and its anticancer effects.
      Yuan Y, Makita N, Cao D, Mihara K, Kadomatsu K, Takei Y.
      Nucleic Acid Ther. 2015 Apr;25(2):85-94. PMID: 25692652
    • Specific inhibition of tumor cells by oncogenic EGFR specific silencing by RNA interference.
      Takahashi M, Chiyo T, Okada T, Hohjoh H.
      PLoS One. 2013 Aug 8;8(8):e73214. PMID: 23951344
    • Targeting Aurora kinase A suppresses the growth of human oral squamous cell carcinoma cells in vitro and in vivo.
      Tanaka H, Nakashiro KI, Iwamoto K, Tokuzen N, Fujita Y, Shirakawa R, Oka R, Goda H, Hamakawa H.
      Oral Oncol. 2013 Jun;49(6):551-9. PMID: 23481312
    • Intraperitoneal delivery of a small interfering RNA targeting NEDD1 prolongs the survival of scirrhous gastric cancer model mice.
      Fujita T, Yanagihara K, Takeshita F, Aoyagi K, Nishimura T, Takigahira M, Chiwaki F, Fukagawa T, Katai H, Ochiya T, Sakamoto H, Konno H, Yoshida T, Sasaki H.
      Cancer Sci. 2013 Feb;104(2):214-22. PMID: 23106787
    • Effect of ADAM28 on Carcinoma Cell Metastasis by Cleavage of von Willebrand Factor.
      Mochizuki S, Soejima K, Shimoda M, Abe H, Sasaki A, Okano HJ, Okano H, Okada Y.
      J Natl Cancer Inst. 2012 Jun 20;104(12):906-22. PMID:22636800
    • siRNA targeting Livin decreases tumor in a xenograft model for colon cancer.
      Oh BY, Lee RA, Kim KH.
      World J Gastroenterol. 2011 May 28;17(20):2563-71. PMID:21633662
    • MicroRNA-143 regulates human osteosarcoma metastasis by regulating matrix metalloprotease-13 expression.
      Osaki M, Takeshita F, Sugimoto Y, Kosaka N, Yamamoto Y, Yoshioka Y, Kobayashi E, Yamada T, Kawai A, Inoue T, Ito H, Oshimura M, Ochiya T.
      Mol Ther. 2011 Jun;19(6):1123-30. PMID:21427707
    • Anti-tumor effect of small interfering RNA targeting the androgen receptor in human androgen-independent prostate cancer cells.
      Azuma K, Nakashiro K, Sasaki T, Goda H, Onodera J, Tanji N, Yokoyama M, Hamakawa H.
      Biochem Biophys Res Commun. 2010 Jan 1;391(1):1075-9. PMID:20004643
    • Rb inactivation accelerates neoplastic growth and substitutes for recurrent amplification of cIAP1, cIAP2 and Yap1 in sporadic mammary carcinoma associated with p53 deficiency.
      Cheng L, Zhou Z, Flesken-Nikitin A, Toshkov IA, Wang W, Camps J, Ried T, Nikitin AY.
      Oncogene. 2010 Oct 21;29(42):5700-11. PMID:20676140
    • Knockdown of Akt isoforms by RNA silencing suppresses the growth of human prostate cancer cells in vitro and in vivo.
      Sasaki T, Nakashiro K, Tanaka H, Azuma K, Goda H, Hara S, Onodera J, Fujimoto I, Tanji N, Yokoyama M, Hamakawa H.
      Biochem Biophys Res Commun. 2010 Aug 13;399(1):79-83. PMID:20638364
    • Systemic delivery of synthetic microRNA-16 inhibits the growth of metastatic prostate tumors via downregulation of multiple cell-cycle genes.
      Takeshita F, Patrawala L, Osaki M, Takahashi RU, Yamamoto Y, Kosaka N, Kawamata M, Kelnar K, Bader AG, Brown D, Ochiya T.
      Mol Ther. 2010 Jan;18(1):181-7. PMID:19738602.
    • Systemic delivery of siRNA specific to tumor mediated by atelocollagen: combined therapy using siRNA targeting Bcl-xL and cisplatin against prostate cancer.
      Mu P, Nagahara S, Makita N, Tarumi Y, Kadomatsu K, Takei Y.
      Int J Cancer. 2009 Dec 15;125(12):2978-90. PMID:19422046
    • Glutathione S-transferase Pi mediates proliferation of androgen-independent prostate cancer cells.
      Hokaiwado N, Takeshita F, Naiki-Ito A, Asamoto M, Ochiya T, Shirai T.
      Carcinogenesis. 2008 Jun;29(6):1134-8. PMID: 18413363
    • Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer.
      Kawata E, Ashihara E, Kimura S, Takenaka K, Sato K, Tanaka R, Yokota A, Kamitsuji Y, Takeuchi M, Kuroda J, Tanaka F, Yoshikawa T, Maekawa T.
      Mol Cancer Ther. 2008 Sep;7(9):2904-12. PMID: 18790771
    • Efficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivo.
      Takeshita F, Minakuchi Y, Nagahara S, Honma K, Sasaki H, Hirai K, Teratani T, Namatame N, Yamamoto Y, Hanai K, Kato T, Sano A, Ochiya T.
      Proc Natl Acad Sci U S A. 2005 Aug 23;102(34):12177-82. PMID:16091473
    • Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine-γ-Lyase-Produced H2S.
      Chen S, Zhang JH, Hu YY, Hu DH, Gao SS, Fan YF, Wang YL, Jiao Y, Chen ZW.
      Evid Based Complement Alternat Med. 2018 May 31;2018:8903849. PMID: 29955237
    • Activator Protein-1 Decoy Oligodeoxynucleotide Transfection Is Beneficial in Reducing Organ Injury and Mortality in Septic Mice.
      Imaizumi T, Matsuda N, Tomita K, Palikhe S, Ohashi W, Hattori K, Hattori Y.
      Crit Care Med. 2018 May;46(5):e435-e442. PMID: 29406423
    • Dissociation of Systemic Glucose Homeostasis from Triacylglyceride Accumulation by Reduced Acsl6 Expression in Skeletal Muscle.
      Lee, J.Y., Kim, AR., Jung, YH. et al.
      Biotechnol Bioproc E (2018) 23: 465.
    • SIRT3 deficiency leads to induction of abnormal glycolysis in diabetic kidney with fibrosis.
      Srivastava SP, Li J, Kitada M, Fujita H, Yamada Y, Goodwin JE, Kanasaki K, Koya D.
      Cell Death Dis. 2018 Sep 24;9(10):997. PMID: 30250024
    • HSF1 deficiency accelerates the transition from pressure overload-induced cardiac hypertrophy to heart failure through endothelial miR-195a-3p-mediated impairment of cardiac angiogenesis.
      Wang S, Wu J, You J, Shi H, Xue X, Huang J, Xu L, Jiang G, Yuan L, Gong X, Luo H, Ge J, Cui Z, Zou Y.
      J Mol Cell Cardiol. 2018 Apr 5;118:193-207. PMID: 29626503
    • Protective Effect of Let-7 miRNA Family in Regulating Inflammation in Diabetes-Associated Atherosclerosis.
      Brennan E, Wang B, McClelland A, Mohan M, Marai M, Beuscart O, Derouiche S, Gray S, Pickering R, Tikellis C, de Gaetano M, Barry M, Belton O, Ali-Shah ST, Guiry P, Jandeleit-Dahm KA, Cooper ME, Godson C, Kantharidis P.
      Diabetes. 2017 Aug;66(8):2266-2277. PMID: 28487436
    • Signal transducer and activator of transcription 5 plays a crucial role in hepatic lipid metabolism through regulation of CD36 expression.
      Hosui A, Tatsumi T, Hikita H, Saito Y, Hiramatsu N, Tsujii M, Hennighausen L, Takehara T.
      Hepatol Res. 2017 Jul;47(8):813-825. PMID: 27593674
    • Activator Protein-1 Decoy Oligodeoxynucleotide Transfection Is Beneficial in Reducing Organ Injury and Mortality in Septic Mice.
      Imaizumi T, Matsuda N, Tomita K, Palikhe S, Ohashi W, Hattori K, Hattori Y.
      Crit Care Med. 2018 May;46(5):e435-e442. PMID: 29406423
    • Deficiency in catechol-o-methyltransferase is linked to a disruption of glucose homeostasis in mice.
      Kanasaki M, Srivastava SP, Yang F, Xu L, Kudoh S, Kitada M, Ueki N, Kim H, Li J, Takeda S, Kanasaki K, Koya D.
      Sci Rep. 2017 Aug 11;7(1):7927. PMID: 28801594
    • MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation.
      Tsukita S, Yamada T, Takahashi K, Munakata Y, Hosaka S, Takahashi H, Gao J, Shirai Y, Kodama S, Asai Y, Sugisawa T, Chiba Y, Kaneko K, Uno K, Sawada S, Imai J, Katagiri H.
      EBioMedicine. 2017 Feb;15:163-172. PMID: 27974246
    • MiR-29a Assists in Preventing the Activation of Human Stellate Cells and Promotes Recovery From Liver Fibrosis in Mice.
      Matsumoto Y, Itami S, Kuroda M, Yoshizato K, Kawada N, Murakami Y.
      Mol Ther. 2016 Oct;24(10):1848-1859. PMID: 27480597
    • Role of Sialidase in Long-Term Potentiation at Mossy Fiber-CA3 Synapses and Hippocampus-Dependent Spatial Memory.
      Minami A, Saito M, Mamada S, Ieno D, Hikita T, Takahashi T, Otsubo T, Ikeda K, Suzuki T.
      PLoS One. 2016 Oct 26;11(10):e0165257. PMID: 27783694
    • MicroRNA-124 inhibits the progression of adjuvant-induced arthritis in rats.
      Nakamachi Y, Ohnuma K, Uto K, Noguchi Y, Saegusa J, Kawano S.
      Ann Rheum Dis. 2016 Mar;75(3):601-8. PMID: 25596157
    • Pivotal Role of Carbohydrate Sulfotransferase 15 in Fibrosis and Mucosal Healing in Mouse Colitis.
      Suzuki K, Arumugam S, Yokoyama J, Kawauchi Y, Honda Y, Sato H, Aoyagi Y, Terai S, Okazaki K, Suzuki Y, Mizumoto S, Sugahara K, Atreya R, Neurath MF, Watanabe K, Hashiguchi T, Yoneyama H, Asakura H.
      PLoS One. 2016 Jul 13;11(7):e0158967. PMID: 27410685
    • Involvement of Hydrogen Sulfide in Endothelium-Derived Relaxing Factor-Mediated Responses in Rat Cerebral Arteries.
      Wang M, Hu Y, Fan Y, Guo Y, Chen F, Chen S, Li Q, Chen Z.
      J Vasc Res. 2016 Oct 13;53(3-4):172-185. PMID: 27732975
    • RNA interference targeting carbohydrate sulfotransferase 3 diminishes macrophage accumulation, inhibits MMP-9 expression and promotes lung recovery in murine pulmonary emphysema.
      Kai Y, Tomoda K, Yoneyama H, Yoshikawa M, Kimura H.
      Respir Res. 2015 Dec 9;16:146. PMID: 26646821
    • Eomesodermin-expressing T-helper cells are essential for chronic neuroinflammation.
      Raveney BJ, Oki S, Hohjoh H, Nakamura M, Sato W, Murata M, Yamamura T.
      Nat Commun. 2015 Oct 5;6:8437. PMID: 26436530
    • MicroRNA-451 down-regulates neutrophil chemotaxis via p38 MAPK.
      Murata K, Yoshitomi H, Furu M, Ishikawa M, Shibuya H, Ito H, Matsuda S.
      Arthritis Rheumatol. 2014 Mar;66(3):549-59. PMID: 24574214
    • Efficient Delivery of siRNA by Atelocollagen in a Murine Laser-Induced Choroidal Neovascularization Model.
      Ito Y, Nozaki M, Sugitani K, Ashikari M, Hirano Y, Ogura Y.
      Ophthalmologica. 2013;230(4):215-21. PMID: 24080835
    • The Downregulation of microRNA let-7a Contributes to the Excessive Expression of Type I Collagen in Systemic and Localized Scleroderma.
      Makino K, Jinnin M, Hirano A, Yamane K, Eto M, Kusano T, Honda N, Kajihara I, Makino T, Sakai K, Masuguchi S, Fukushima S, Ihn H.
      J Immunol. 2013 Apr 15;190(8):3905-15. PMID: 23509348
    • Nuclear Receptor NR4A2 Orchestrates Th17 Cell-Mediated Autoimmune Inflammation via IL-21 Signalling.
      Raveney BJ, Oki S, Yamamura T.
      PLoS One. 2013;8(2):e56595. PMID: 23437182
    • Effects of Atelocollagen Formulation Containing Oligonucleotide on Endothelial Permeability.
      Koji Hanai, Takashi Kojima, Mika Ota, Jun Onodera, and Norimasa Sawada, Journal of J Drug Deliv. 2012;2012:245835. PMID: 22506120
    • Atelocollagen-mediated Systemic Delivery Prevents Immunostimulatory Adverse Effects of siRNA in Mammals.
      Inaba S, Nagahara S, Makita N, Tarumi Y, Ishimoto T, Matsuo S, Kadomatsu K, Takei Y.
      Mol Ther. 2012 Feb;20(2):356-66 PMID:22031237
    • Manipulation of CD98 Resolves Type 1 Diabetes in NOD Mice.
      Lian G, Arimochi H, Kitamura A, Nishida J, Li S, Kishihara K, Maekawa Y, Yasutomo K.
      J Immunol. 2012 Mar 1;188(5):2227-34 PMID:22291182
    • MicroRNA-181b regulates NF-κB-mediated vascular inflammation.
      Sun X, Icli B, Wara AK, Belkin N, He S, Kobzik L, Hunninghake GM, Vera MP, Blackwell TS, Baron RM, Feinberg MW.
      J Clin Invest. 2012 Jun 1;122(6):1973-90. PMID:22622040
    • Reduction of Type IV Collagen by Upregulated miR-29 in Normal Elderly Mouse and klotho-Deficient, Senescence-Model Mouse.
      Takahashi M, Eda A, Fukushima T, Hohjoh H.
      PLoS One. 2012;7(11):e48974. PMID: 23139829
    • Mechanisms of the hepatoprotective effects of tamoxifen against drug-induced and chemical-induced acute liver injuries.
      Yoshikawa Y, Miyashita T, Higuchi S, Tsuneyama K, Endo S, Tsukui T, Toyoda Y, Fukami T, Nakajima M, Yokoi T.
      Toxicol Appl Pharmacol. 2012 Oct 1;264(1):42-50. PMID:22841776
    • Atelocollagen-mediated systemic administration of myostatin-targeting siRNA improves muscular atrophy in caveolin-3-deficient mice.
      Kawakami E, Kinouchi N, Adachi T, Ohsawa Y, Ishimaru N, Ohuchi H, Sunada Y, Hayashi Y, Tanaka E, Noji S.
      Dev Growth Differ. 2011 Jan;53(1):48-54. PMID:21261610
    • The inhibitory effect of microRNA-146 expression on bone destruction in arthritis.
      Nakasa T, Shibuya H, Nagata Y, Niimoto T, Ochi M.
      Arthritis Rheum. 2011 Jun;63(6):1582-90.  PMID:21425254
    • Chondroitin sulphate N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury.
      Takeuchi K, Yoshioka N, Higa Onaga S, Watanabe Y, Miyata S, Wada Y, Kudo C, Okada M, Ohko K, Oda K, Sato T, Yokoyama M, Matsushita N, Nakamura M, Okano H, Sakimura K, Kawano H, Kitagawa H, Igarashi M.
      Nat Commun. 2013;4:2740. PMID: 24220492
    • Cathepsin L inhibition prevents murine autoimmune diabetes via suppression of CD8(+) T cell activity.
      Yamada A, Ishimaru N, Arakaki R, Katunuma N, Hayashi Y.
      PLoS One. 2010 Sep 22;5(9):e12894. PMID:20877570
    • Downregulation of monocyte chemoattractant protein-1 involving short interfering RNA attenuates hapten-induced contact hypersensitivity.
      Ishimoto T, Takei Y, Yuzawa Y, Hanai K, Nagahara S, Tarumi Y, Matsuo S, Kadomatsu K.
      Mol Ther. 2008 Feb;16(2):387-95. PMID:18059372
    • Atelocollagen-mediated local and systemic applications of myostatin-targeting siRNA increase skeletal muscle mass.
      Kinouchi N, Ohsawa Y, Ishimaru N, Ohuchi H, Sunada Y, Hayashi Y, Tanimoto Y, Moriyama K, Noji S.
      Gene Ther. 2008 Aug;15(15):1126-30. PMID:18323791
    • Potential of atelocollagen-mediated systemic antisense therapeutics for inflammatory disease.
      Hanai K, Kurokawa T, Minakuchi Y, Maeda M, Nagahara S, Miyata T, Ochiya T, Sano A.
      Hum Gene Ther. 2004 Mar;15(3):263-72. PMID:15018735