Atelocollagen Powder, Membrane, Sponge | KOKEN CO., LTD. | KOKEN CO., LTD.

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  • CLP-01

  • CLF-01

  • CLS-01

Atelocollagen Powder, Membrane, Sponge


High purity bovine dermis-derived atelocollagen powder, permeable membrane, and sponge for various research purposes. Useful for scaffold research.

What is Atelocollagen?


  • Preparing high concentration solution

  • Processing permeable membrane

  • Cross-linking

Types of collagen

Bovine dermis derived atelocollagen

Cat. No. Product Packaging Storage Price
CLP-01 Atelocollagen Powder 500 mg/bottle -20℃ Visit distributor’s
web page
CLF-01 Atelocollagen Membrane
100mm × 90mm × 35μm
1 sheet/box -20℃ Visit distributor’s
web page

Atelocollagen Sponge*
90mm × 80mm × 5mm
*NOT cross-linked and will deform
after water absorption

1 piece/box 2-10℃ Visit distributor’s
web page

For research use only.

Click here for frequently asked questions about Atelocollagen Powder, Membrane, Sponge



  • Atelocollagen powder
  • Atelocollagen membrane
  • Atelocollagen sponge (NOT cross-linked)
    • PDMS microstencil plate-supported fabrication of ultra-thin, condensed ECM membranes for separated cell coculture on both surfaces.
      Hideki Iwadate, Masumi Yamada, Naoki Kimura, Rina Hashimoto, Yuya Yajima, Rie Utoh, Minoru Seki.
      Sensors and Actuators B: Chemical. Volume 287, 2019, Pages 486-495.
    • Effects of structurally stabilized EGF and bFGF on wound healing in type I and type II diabetic mice.
      Choi SM, Lee KM, Kim HJ, Park IK, Kang HJ, Shin HC, Baek D, Choi Y, Park KH, Lee JW.
      Acta Biomater. 2018 Jan 15;66:325-334. PMID: 29203426
    • Investigation of the Extracellular Matrix Effect for the QCM/CCD Cell Activity Monitoring System.
      Kang HW, Otani N, Hiroshi M, Chang SM, Kim JM.
      J Nanosci Nanotechnol. 2018 Aug 1;18(8):5777-5784. PMID: 29458639
    • Recombinant human IGF-1 produced by transgenic plant cell suspension culture enhances new bone formation in calvarial defects.
      Poudel SB, Bhattarai G, Kook SH, Shin YJ, Kwon TH, Lee SY, Lee JC.
      Growth Horm IGF Res. 2017 Jul 30;36:1-10. PMID: 28787635
    • Multi-layered cell assembling technology using dielectrophoresis and construction of skin tissue microelement
      Transactions of the JSME (in Japanese) Vol. 83 (2017) No. 848 p. 16-00387
    • COMP-Ang1 prevents periodontitic damages and enhances mandible bone growth in an experimental animal model.
      Bhattarai G, Kook SH, Kim JH, Poudel SB, Lim SS, Seo YK, Lee JC.
      Bone. 2016 Nov;92:168-179. PMID: 27612438
    • Development of Stabilized Growth Factor-Loaded Hyaluronate- Collagen Dressing (HCD) matrix for impaired wound healing.
      Choi SM, Ryu HA, Lee KM, Kim HJ, Park IK, Cho WJ, Shin HC, Choi WJ, Lee JW.
      Biomater Res. 2016 Apr 1;20:9. PMID: 27042331
    • Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering.
      Dhand C, Ong ST, Dwivedi N, Diaz SM, Venugopal JR, Navaneethan B, Fazil MH, Liu S, Seitz V,Wintermantel E, Beuerman RW, Ramakrishna S, Verma NK, Lakshminarayanan R.
      Biomaterials. 2016 Oct;104:323-38. PMID: 27475728
    • Hesperidin interaction to collagen detected by physico-chemical techniques.
      Hiraishi N, Maruno T, Tochio N, Sono R, Otsuki M, Takatsuka T, Tagami J, Kobayashi Y.
      Dent Mater. 2016 Oct 19. pii: S0109-5641(16)30483-3. PMID: 27771138
    • Minocycline Hydrochloride Entrapped Biomimetic Nanofibrous Substitutes for Adipose-Derived Stem Cells Differentiation into Osteogenesis.
      Jayaraman, P., Gandhimathi, C., Venugopal, J.R. et al.
      Regen. Eng. Transl. Med. (2016) Volume 2, Issue 1, pp 10–22.
    • Nanoengineered biocomposite tricomponent polymer based matrices for bone tissue engineering.
      Ewa Kijeńska, Sai Zhang, Molamma P. Prabhakaran, Seeram Ramakrishna, and Wojciech Swieszkowski
      International Journal of Polymeric Materials and Polymeric Biomaterials, 65:16, 807-815.
    • Collagen Hydrogel Scaffold and Fibroblast Growth Factor-2 Accelerate Periodontal Healing of Class II Furcation Defects in Dog.
      Momose T, Miyaji H, Kato A, Ogawa K, Yoshida T, Nishida E, Murakami S, Kosen Y, Sugaya T, Kawanami M.
      Open Dent J. 2016 Jul 29;10:347-59. PMID: 27583044
    • Local delivery of recombinant human FGF7 enhances bone formation in rat mandible defects.
      Poudel SB, Bhattarai G, Kim JH, Kook SH, Seo YK, Jeon YM, Lee JC.
      J Bone Miner Metab. 2017 Sep;35(5):485-496. PMID: 27766421
    • Functionalization of the Polymeric Surface with Bioceramic Nanoparticles via a Novel, Nonthermal Dip Coating Method.
      Riau AK, Mondal D, Setiawan M, Palaniappan A, Yam GH, Liedberg B, Venkatraman SS, Mehta JS.
      ACS Appl Mater Interfaces. 2016 Dec 28;8(51):35565-35577. PMID: 27966877
    • Co-effect of silk and amniotic membrane for tendon repair.
      Seo YK, Kim JH, Eo SR.
      J Biomater Sci Polym Ed. 2016 Aug;27(12):1232-47. PMID: 27188627
    • Collagen-Based Artificial Corneal Scaffold with Anti-Infective Capability for Prevention of Perioperative Bacterial Infections.
      Andri K. Riau, Debasish Mondal, Thet T. Aung, Elavazhagan Murugan, Liyan Chen, Nyein C. Lwin, Lei Zhou, Roger W. Beuerman, Bo Liedberg, Subbu S. Venkatraman, and Jodhbir S. Mehta
      ACS Biomaterials Science & Engineering 2015 1 (12), 1324-1334.
    • Quantitative imaging of electrospun fibers by PeakForce Quantitative NanoMechanics atomic force microscopy using etched scanning probes.
      Chlanda A, Rebis J, Kijeńska E, Wozniak MJ, Rozniatowski K, Swieszkowski W, Kurzydlowski KJ.
      Micron. 2015 May;72:1-7. PMID: 25710786
    • Lipid decomposition by sonic vibration in matured 3T3-L1 cells within 3-Dimensional artificial adipose.
      Hyunjin Cho, Eun-Seon Oh, Young-Kwon Seo, Hee-Hoon Yoon, Moon-Young Yoon, Jung-Keug Park
      Biotechnology and Bioprocess Engineering. Volume 20, Issue 1 , pp 188-193.
    • Molecular level evaluation on HEMA interaction with a collagen model.
      Hiraishi N, Tochio N, Kigawa T, Otsuki M, Tagami J.
      Dent Mater. 2015 Feb;31(2):88-92. PMID: 25499247
    • Local delivery of COMP-angiopoietin 1 accelerates new bone formation in rat calvarial defects.
      Lim SS, Kook SH, Bhattarai G, Cho ES, Seo YK, Lee JC.
      J Biomed Mater Res A. 2015 Sep;103(9):2942-51. PMID: 25727390
    • Pilot study of gingival connective tissue responses to 3-dimensional collagen nanofiber-coated dental implants.
      Raita Y, Komatsu K, Hayakawa T.
      Dent Mater J. 2015;34(6):847-54. PMID: 26632234
    • Use of biomimetic microtissue spheroids and specific growth factor supplementation to improve tenocyte differentiation and adaptation to a collagen-based scaffold in vitro.
      Theiss F, Mirsaidi A, Mhanna R, Kümmerle J, Glanz S, Bahrenberg G, Tiaden AN, Richards PJ.
      Biomaterials. 2015 Nov;69:99-109. PMID: 26283157
    • Role of 2-hydroxyethyl methacrylate in the interaction of dental monomers with collagen studied by saturation transfer difference NMR.
      Hiraishi N, Tochio N, Kigawa T, Otsuki M, Tagami J.
      J Dent. 2014 Apr;42(4):484-9. PMID: 24440604
    • Guiding the orientation of smooth muscle cells on random and aligned polyurethane/collagen nanofibers.
      Jia L, Prabhakaran MP, Qin X, Ramakrishna S.
      J Biomater Appl. 2014 Sep;29(3):364-77. PMID: 24682037
    • The effect of dose on rhBMP-2 signaling, delivered via collagen sponge, on osteoclast activation and in vivo bone resorption.
      Kim RY, Oh JH, Lee BS, Seo YK, Hwang SJ, Kim IS.
      Biomaterials. 2014 Feb;35(6):1869-81. PMID: 24321706
    • Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells.
      Liao S, Nguyen LT, Ngiam M, Wang C, Cheng Z, Chan CK, Ramakrishna S.
      Adv Healthc Mater. 2014 May;3(5):737-51. PMID: 24574245
    • Differentiation of embryonic stem cells to cardiomyocytes on electrospun nanofibrous substrates.
      Prabhakaran MP, Mobarakeh LG, Kai D, Karbalaie K, Nasr-Esfahani MH, Ramakrishna S.
      J Biomed Mater Res B Appl Biomater. 2014 Apr;102(3):447-54. PMID: 24039141
    • Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation.
      Rashid R, Lim NS, Chee SM, Png SN, Wohland T, Raghunath M.
      Tissue Eng Part C Methods. 2014 Dec;20(12):994-1002. PMID: 24665935
    • Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells.
      Xue C, Kwek K, Chan JK, Chan CK, Lim M.
      J Biomed Mater Res A. 2014 Feb;102(2):523-31. PMID: 23536515
    • Trans-differentiation of human mesenchymal stem cells generates functional hepatospheres on poly(L-lactic acid)-co-poly(ε-caprolactone)/collagen nanofibrous scaffolds.
      Dillip Kumar Bishi, Santosh Mathapati, Jayarama Reddy Venugopal, Soma Guhathakurta, Kotturathu Mammen Cherian, Seeram Ramakrishna and Rama Shanker Verma
      J. Mater. Chem. B, 2013,1, 3972-3984.
    • Mimicking nanofibrous hybrid bone substitute for mesenchymal stem cells differentiation into osteogenesis.
      Gandhimathi C, Venugopal J, Ravichandran R, Sundarrajan S, Suganya S, Ramakrishna S.
      Macromol Biosci. 2013 Jun;13(6):696-706. PMID: 23529905
    • Monomer-Collagen Interactions Studied by Saturation Transfer Difference NMR.
      Hiraishi N, Tochio N, Kigawa T, Otsuki M, Tagami J.
      J Dent Res. 2013 Mar;92(3):284-8. PMID: 23340212
    • Electrospun Aligned PHBV/Collagen Nanofibers as Substrates for Nerve Tissue Engineering.
      Prabhakaran MP, Vatankhah E, Ramakrishna S.
      Biotechnol Bioeng. 2013 Oct;110(10):2775-84. PMID: 23613155
    • Electrosprayed hydroxyapatite on polymer nanofibers to differentiate mesenchymal stem cells to osteogenesis.
      Venugopal J, Rajeswari R, Shayanti M, Low S, Bongso A, Dev VR, Deepika G, Choon AT, Ramakrishna S.
      J Biomater Sci Polym Ed. 2013;24(2):170-84. PMID: 22370175
    • Human umbilical cord Wharton's jelly stem cells undergo enhanced chondrogenic differentiation when grown on nanofibrous scaffolds and in a sequential two-stage culture medium environment.
      Fong CY, Subramanian A, Gauthaman K, Venugopal J, Biswas A, Ramakrishna S, Bongso A.
      Stem Cell Rev. 2012 Mar;8(1):195-209. PMID:21671058
    • Enhanced osteogenic differentiation with 3D electrospun nanofibrous scaffolds.
      Nguyen LT, Liao S, Chan CK, Ramakrishna S.
      Nanomedicine (Lond). 2012 7(10):1561-75. PMID:22709343
    • Composite poly-L-lactic acid/poly-(α,β)-DL-aspartic acid/collagen nanofibrous scaffolds for dermal tissue regeneration.
      Ravichandran R, Venugopal JR, Sundarrajan S, Mukherjee S, Sridhar R, Ramakrishna S.
      Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1443-51. PMID: 24364944
    • Biomimetic surface modification of titanium surfaces for early cell capture by advanced electrospinning.
      Ravichandran R, Ng CCh, Liao S, Pliszka D, Raghunath M, Ramakrishna S, Chan CK.
      Biomed Mater. 2012 Feb;7(1):015001. PMID:22156014
    • Precipitation of nanohydroxyapatite on PLLA/PBLG/Collagen nanofibrous structures for the differentiation of adipose derived stem cells to osteogenic lineage.
      Ravichandran R, Venugopal JR, Sundarrajan S, Mukherjee S, Ramakrishna S.
      Biomaterials. 2012 Jan;33(3):846-55. PMID:22048006
    • Osteogenic differentiation of human Wharton's jelly stem cells on nanofibrous substrates in vitro.
      Gauthaman K, Venugopal JR, Yee FC, Biswas A, Ramakrishna S, Bongso A.
      Tissue Eng Part A. 2011 Jan;17(1-2):71-81. PMID:20673136
    • Effects of nanofiber/stem cell composite on wound healing in acute full-thickness skin wounds.
      Ma K, Liao S, He L, Lu J, Ramakrishna S, Chan CK.
      Tissue Eng Part A. 2011 May;17(9-10):1413-24. PMID: 21247260
    • Effects of mechanical stimulation in osteogenic differentiation of bone marrow-derived mesenchymal stem cells on aligned nanofibrous scaffolds.
      Ngiam, M., Liao, S., Jie, T. O. J., Sui, X., Dong, Y., Ramakrishna, S., & Chan, C. K.
      J Bioact Compat Pol. January 2011 vol. 26 no. 1 56-70.
    • Comparison of hair dermal cells and skin fibroblasts in a collagen sponge for use in wound repair.
      Youn-Ho Shin, Young-Kwon Seo, Hee-Hoon Yoon, Bo-Young Yoo, Kye-Yong Song and Jung-Keug Park
      Biotechnol Bioproc E. 2011 Volume 16, Number 4, 793-800.
    • Collagen Nanofiber on Titanium or Partially Stabilized Zirconia by Electrospray Deposition.
      Hayakawa T, Yoshinari M, Nitta K, Inoue K.
      J Hard Tissue Biol. 2010. 19(1):5-12.
    • Composite nanofiber mats consisting of hydroxyapatite and titania for biomedical applications.
      Kim HM, Chae WP, Chang KW, Chun S, Kim S, Jeong Y, Kang IK.
      J Biomed Mater Res B Appl Biomater. 2010 Aug;94(2):380-7. PMID: 20574975
    • Bone Augmentation by Implantation of an FGF2-loaded Collagen Gel-sponge Composite Scaffold.
      Naoko KOBAYASHI, Hirofumi MIYAJI, Tsutomu SUGAYA and Masamitsu KAWANAMI.
      Journal of Oral Tissue Engineering Vol. 8 (2010) , No. 2 pp.91-101.
    • Fluorescence lifetime probe for solvent microviscosity utilizing anilinonaphthalene sulfonate.
      Someya Y, Yui H.
      Anal Chem. 2010. 1;82(13):5470-6. PMID: 20524653
    • Enhanced biomineralization in osteoblasts on a novel electrospun biocomposite nanofibrous substrate of hydroxyapatite/collagen/chitosan.
      Zhang Y, Reddy VJ, Wong SY, Li X, Su B, Ramakrishna S, Lim CT.
      Tissue Eng Part A. 2010. 16(6):1949-60. PMID: 20088700
    • Early adhesive behavior of bone-marrow-derived mesenchymal stem cells on collagen electrospun fibers.
      Chan CK, Liao S, Li B, Lareu RR, Larrick JW, Ramakrishna S, Raghunath M.
      Biomed Mater. 2009. 4(3):035006. PMID: 19439824
    • Nanofibrous substrates support colony formation and maintain stemness of human embryonic stem cells.
      Gauthaman K, Venugopal JR, Yee FC, Peh GS, Ramakrishna S, Bongso A
      J Cell Mol Med. 2009 Sep;13(9B):3475-84. PMID: 19228268
    • The fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nanofibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering.
      Ngiam M, Liao S, Patil AJ, Cheng Z, Chan CK, Ramakrishna S.
      Bone. 2009 Jul;45(1):4-16. PMID: 19358900
    • Increase in cell migration and angiogenesis in a composite silk scaffold for tissue-engineered ligaments.
      Seo YK, Yoon HH, Song KY, Kwon SY, Lee HS, Park YS, Park JK.
      J Orthop Res. 2009 Apr;27(4):495-503. PMID: 18924141
    • Fabrication of nano-hydroxyapatite/collagen/osteonectin composites for bone graft applications.
      Liao S, Ngiam M, Chan CK, Ramakrishna S.
      Biomed Mater. 2009 Apr;4(2):025019. PMID: 19349652
    • Fabrication of mineralized polymeric nanofibrous composites for bone graft materials.
      Ngiam M, Liao S, Patil AJ, Cheng Z, Yang F, Gubler MJ, Ramakrishna S, Chan CK.
      Tissue Eng Part A. 2009 Mar;15(3):535-46. PMID: 18759670
    • Processing nanoengineered scaffolds through electrospinning and mineralization suitable for biomimetic bone tissue engineering.
      Liao S, Murugan R, Chan CK, Ramakrishna S.
      J Mech Behav Biomed Mater. 2008 Jul;1(3):252-60. PMID: 19627790
    • Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells.
      Ma K, Chan CK, Liao S, Hwang WY, Feng Q, Ramakrishna S.
      Biomaterials. 2008 May;29(13):2096-103. PMID: 18289666
    • Reinforced bioartificial dermis constructed with collagen threads.
      YK Seo, HH Youn, CS Park, KY Song, JK Park.
      Biotechnol Bioproc E. 2008 Volume 13, Issue 6 , pp 745-751.
    • Remodeling of Three-dimensional Hierarchically Organized Nanofibrous Assemblies.
      W.E. Teo W.E., Liao S., Chan C.K., Ramakrishna S.
      Curr Nanosci. 2008, 4, 361-369.
    • Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note.
      Zeugolis DI, Li B, Lareu RR, Chan CK, Raghunath M.
      J Biomater Sci Polym Ed. 2008;19(10):1307-17. PMID: 18854124
    • Wound healing effect of acellular artificial dermis containing extracellular matrix secreted by human skin fibroblasts.
      Seo YK, Song KY, Kim YJ, Park JK.
      Artif Organs. 2007 Jul;31(7):509-20. PMID: 17584475
    • Development of a cultured dermal substitute composed of a spongy matrix of hyaluronic acid and atelo-collagen combined with fibroblasts: cryopreservation.
      Kubo K, Kuroyanagi Y.
      Artif Organs. 2004 Feb;28(2):182-8. PMID: 14961958
    • Preparation and characteristics of hybrid scaffolds composed of beta-chitin and collagen.
      Lee SB, Kim YH, Chong MS, Lee YM.
      Biomaterials. 2004 May;25(12):2309-17. PMID: 14741596
    • Collagen reversible denaturation by a weak near-infrared laser light irradiation for vascular softening.
      M. Kobayashi, M. Kobayashi, E. Ogawa, E. Ogawa, T. Arai, T. Arai.
      Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108202G (23 October 2018).
    • Portable Micropatterns of Neuronal Cells Supported by Thin Hydrogel Films
      Kuniaki Nagamine , Takuya Hirata , Kohei Okamoto , Yuina Abe , Hirokazu Kaji , and Matsuhiko Nishizawa
      ACS Biomater. Sci. Eng., 2015, 1 (5), pp 329–334.
    • Designing of a Si-MEMS device with an integrated skeletal muscle cell-based bio-actuator.
      Fujita H, Van Dau T, Shimizu K, Hatsuda R, Sugiyama S, Nagamori E.
      Biomed Microdevices. 2011 Feb;13(1):123-9. PMID: 20957437
    • Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
      Fujita H, Endo A, Shimizu K, Nagamori E.
      Biotechnol Bioeng. 2010 Dec 1;107(5):894-901. PMID: 20635352
    • Rapid decrease in active tension generated by C2C12 myotubes after termination of artificial exercise.
      Fujita H, Hirano M, Shimizu K, Nagamori E.
      J Muscle Res Cell Motil. 2010 Dec;31(4):279-88. PMID: 21120590
    • Novel method for measuring active tension generation by C2C12 myotube using UV-crosslinked collagen film.
      Fujita H, Shimizu K, Nagamori E.
      Biotechnol Bioeng. 2010 Jun 15;106(3):482-489. PMID: 20178119
    • Fabrication of scaffold-free contractile skeletal muscle tissue using magnetite-incorporated myogenic C2C12 cells.
      Fujita H, Shimizu K, Yamamoto Y, Ito A, Kamihira M, Nagamori E.
      J Tissue Eng Regen Med. 2010 Aug;4(6):437-443. PMID: 20084621
    • Electroreduction of nitrite to nitrogen oxide by a copper-containing nitrite reductase model complex incorporated into collagen film.
      Isoda N, Yokoyama H, Nojiri M, Suzuki S, Yamaguchi K.
      Bioelectrochemistry. 2010 Feb;77(2):82-88. PMID: 19616484
    • Cell delivery: intramyocardial injections or epicardial deposition? A head-to-head comparison.
      Hamdi H, Furuta A, Bellamy V, Bel A, Puymirat E, Peyrard S, Agbulut O, Menasché P.
      Pluripotent Stem Cell Biology - Advances in Mechanisms, Methods and Models. Prof. Craig Atwood (Ed.), ISBN: 978-953-51-1590-8, InTech, DOI: 10.5772/58394.
    • Cultured mucosal cell sheet with a double layer of keratinocytes and fibroblasts on a collagen membrane.
      Imaizumi F, Asahina I, Moriyama T, Ishii M, Omura K
      Tissue Eng. 2004 May-Jun;10(5-6):657-664. PMID: 15265283
    • Conditioned medium from periodontal ligament stem cells enhances periodontal regeneration.
      Nagata M, Iwasaki K, Akazawa K, Komaki M, Yokoyama N, Izumi Y, Morita I.
      Tissue Eng Part A. 2017 May;23(9-10):367-377. PMID: 28027709
    • Peripheral nerve regeneration through alginate gel: analysis of early outgrowth and late increase in diameter of regenerating axons.
      Hashimoto T, Suzuki Y, Kitada M, Kataoka K, Wu S, Suzuki K, Endo K, Nishimura Y, Ide C.
      Exp Brain Res. 2002 Oct;146(3):356-368. PMID: 12232692