Atelocollagen Sponge MIGHTY scaffold for bone and cartilage research | Koken Co. Ltd.

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Atelocollagen Sponge, MIGHTY

Outline

Atelocollagen sponge MIGHTY consists primarily of type I atelocollagen derived from bovine dermis and can withstand compressive loadings of up to 40 kPa. Culturing cells in MIGHTY under cyclic compressive loading simulates the in vivo environment for evaluating cell function. MIGHTY is also useful as a scaffold for  3D culture.

Applications

  • 3D-culture-in-Mighty

    3D culture

  • Mechanical stress culture

  • Cell transplantation

  • Sustained release carrier

Features

  • Retains structural rigidity during repeated compressive loadings of 40 kPa.

  • Sized for 96-well plates.

  • It is biodegradable and suitable for cell transplantation

Type of collagen

Bovine hide derived atelocollagen

  • Cross-sectional view of an SEM image of MIGHTY

  • Cross-sectional view image of NIH3T3 fibroblasts, seeded in a MIGHTY
    (live cell imaging by fluorescence microscopy)

Cat. No. Product Packaging Price
CSM-25 Atelocollagen Sponge, MIGHTY Sterilized
φ5×3mm		 25 pcs/bottle Visit distributor’s
web page
CSM-50 Atelocollagen Sponge, MIGHTY Sterilized
φ5×3mm		 50 pcs/bottle Visit distributor’s
web page

Storage: Room temperature  For research use only.

Click here for frequently asked questions about MIGHTY

Use case

References

  • Bone, Tooth, Cartilage
  • Skin, Miscellaneous
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      Calcif Tissue Int. 2024 Aug;115(2):185-195. PMID: 38809297.
    • Tacrolimus, FK506, promotes bone formation in bone defect mouse model.
      Nishida S, Azetsu Y, Chatani M, Karakawa A, Otake K, Sugiki H, Sakai N, Maruoka Y, Myers M, Takami M.
      J Oral Biosci. 2024 Feb 13:S1349-0079(24)00016-1. PMID: 38360372.
    • Cyclic compressive loading induces a mature meniscal cell phenotype in mesenchymal stem cells with an atelocollagen-based scaffold.
      Oyama S, Kanamoto T, Ebina K, Etani Y, Hirao M, Goshima A, Otani S, Hikida M, Yamakawa S, Ito S, Okada S, Nakata K.
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    • Reversal of Hyperglycemia by Subcutaneous Islet Engraftment Using an Atelocollagen Sponge as a Scaffold.
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    • Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model.
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    • Effect of compression on mandibular fracture haematoma-derived cells.
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      Putranti NAR, Kunimatsu R, Rikitake K, Hiraki T, Nakajima K, Abe T, Tsuka Y, Sakata S, Nakatani A, Nikawa H, Tanimoto K.
      Cells. 2022 Jun 13;11(12):1914. PMID: 35741043.
    • Cyclic compressive mechanical loading on three­dimensional cultured tissue of human chondrocytes synergistically upregulates MMP­-3 gene expression with IL­-1β.
      Minami HIKIDA, Masahiro NAKAJIMA, Ken NAKATA,
      Journal of Osaka Dental University, 2021, 55 巻, 1 号, p. 91-98.
    • Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel.
      Kanamoto T, Hikida M, Sato S, Oyama S, Tachi Y, Kuroda S, Mazuka T, Ebina K, Nakai T, Nakata K.
      Sci Rep. 2021 Jan 19;11(1):1757. PMID: 33469078
    • Axial mechanical loading to ex vivo mouse long bone regulates endochondral ossification and endosteal mineralization through activation of the BMP-Smad pathway during postnatal growth.
      Miyamoto S, Yoshikawa H, Nakata K.
      Bone Rep. 2021 May 7;15:101088. PMID: 34141832
    • Osteogenic Response to Polysaccharide Nanogel Sheets of Human Fibroblasts After Conversion Into Functional Osteoblasts by Direct Phenotypic Cell Reprogramming.
      Nakai K, Yamamoto K, Kishida T, Kotani SI, Sato Y, Horiguchi S, Yamanobe H, Adachi T, Boschetto F, Marin E, Zhu W, Akiyoshi K, Yamamoto T, Kanamura N, Pezzotti G, Mazda O.
      Front Bioeng Biotechnol. 2021 Sep 3;9:713932. PMID: 34540813.
      線維芽細胞をマイティーに播種し、骨芽細胞へ直接分化。
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      Somemura S, Kumai T, Yatabe K, Sasaki C, Fujiya H, Niki H, Yudoh K.
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      Takeda Y, Niki Y, Fukuhara Y, Fukuda Y, Udagawa K, Shimoda M, Kikuchi T, Kobayashi S, Harato K, Miyamoto T, Matsumoto M, Nakamura M.
      BMC Musculoskelet Disord. 2021 Mar 1;22(1):238. PMID: 33648469
    • Neural crest-derived cells in nasal conchae of adult mice contribute to bone regeneration.
      Yoshida H, Suzawa T, Shibata Y, Takahashi M, Kawai R, Takami M, Maki K, Kamijo R.
      Biochem Biophys Res Commun. 2021 Mar 30;554:173-178. PMID: 33798944.
    • Stem cell-derived conditioned media from human exfoliated deciduous teeth promote bone regeneration.
      Hiraki T, Kunimatsu R, Nakajima K, Abe T, Yamada S, Rikitake K, Tanimoto K.
      Oral Dis. 2020 Mar;26(2):381-390. PMID: 31808229
    • ROCK inhibitors enhance bone healing by promoting osteoclastic and osteoblastic differentiation.
      Nakata J, Akiba Y, Nihara J, Thant L, Eguchi K, Kato H, Izumi K, Ohkura M, Otake M, Kakihara Y, Saito I, Saeki M.
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      Sato M, Aoki H, Nakamura T, Onodera S, Yamaguchi A, Saito A, Azuma T.
      J Periodontal Res. 2020 Oct;55(5):734-743. PMID: 32583900
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      Xiang C, Tenkumo T, Ogawa T, Kanda Y, Nakamura K, Shirato M, Sokolova V, Epple M, Kamano Y, Egusa H, Sasaki K.
      Acta Biomater. 2021 Jan 1;119:375-389. PMID: 33166711.
    • Effect of the combined use of enamel matrix derivative and atelocollagen sponge scaffold on osteoblastic differentiation of mouse induced pluripotent stem cells in vitro.
      Hisanaga Y, Suzuki E, Aoki H, Sato M, Saito A, Saito A, Azuma T.
      J Periodontal Res. 2018 Apr;53(2):240-249. PMID: 29044527
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      Kato T, Khanh VC, Sato K, Kimura K, Yamashita T, Sugaya H, Yoshioka T, Mishima H, Ohneda O.
      Stem Cells Dev. 2018 Jan 15;27(2):85-99. PMID: 29084466
    • Effect of cyclic compressive loading on redifferentiation of human chondrocytes in three-dimensional cultured tissue.
      Tomoko Okamoto, Kenji Kakudo, Yuichi Ohnishi, Masahiro Nakajima, Ken nakata
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      Sci Adv. 2017 May 12;3(5):e1602875. PMID: 28508073
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      Nakamura T, Shirakata Y, Shinohara Y, Miron RJ, Furue K, Noguchi K.
      Clin Oral Investig. 2017 Jun;21(5):1659-1665. PMID: 27726024
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      Shinohara Y, Nakamura T, Shirakata Y, Noguchi K.
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    • Bone formation by human umbilical cord perivascular cells.
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      J Biomed Mater Res A. 2015 Aug;103(8):2807-14. PMID: 25676366
    • Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge.
      Nishio Akihiro, Kubo Hirohito, Kishimoto Naotaka, Hashimoto Yoshiya, Kakudo Kenji.
      J Osaka Dent Univ. 2015 (October);49(2), 185-196.
    • Celecoxib down-regulates mechanically induced ADAMTS-4 gene expression in 3D cultured tissue of human synovium-derived cells at lower concentration than indomethacin.
      M Kondo, K Nakata, K Kakudo.
      J Osaka Dent Univ. 2014 April; 48(1), 55-59.
    • Biodistribution of locally or systemically transplanted osteoblast-like cells.
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    • Cyclic compressive loading on 3D tissue of human synovial fibroblasts upregulates prostaglandin E2 via COX-2 production without IL-1β and TNF-α.
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      J Osaka Dent Univ. 2013 (April);47(1):117-125.
    • Enhanced in vivo osteogenesis by nanocarrier-fused bone morphogenetic protein-4.
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    • Prolonged matrix metalloproteinase-3 high expression after cyclic compressive load on human synovial cells in three-dimensional cultured
      tissue.
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    • Cyclic mechanical loading of human synovial cells in three-dimensional cultured tissue up-regulates gene expressions of inflammatory cytokines and matrix metalloproteinases.
      Keisuke Ota, Yuri Muroi, Kenji Kakudo, Ken Nakata.
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    • Effects of Compressive Loading on Human Synovium-derived cells.
      Muroi Y, Kakudo K, Nakata K.
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    • A study on optimum orthodontic force using human periodontal ligament cells on 3D culture system.
      Meri Haniu, Takenori Kim, Yoshiaki Satho and Junichiro Ida.
      Hokkaido J. Dent. Sci., 34：97-105，2014.
    • Tumor associated osteoclast-like giant cells promote tumor growth and lymphangiogenesis by secreting vascular endothelial growth factor-C.
      Hatano Y, Nakahama KI, Isobe M, Morita I.
      Biochem Biophys Res Commun. 2014 Mar 28;446(1):149-54. PMID: 24607909
    • Effect of strain on human dermal fibroblasts in a three-dimensional collagen sponge.
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      Cytotechnology. 2014 Oct;66(5):723-8. PMID: 24096838
    • Biological responses of three-dimensional cultured fibroblasts by sustained compressive loading include apoptosis and survival activity.
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      PLoS One. 2014 Aug 7;9(8):e104676. PMID: 25102054