Atelocollagen Neutral Solutions for sandwich/on-gel culture | Koken Co., Ltd.

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Atelocollagen Neutral Solutions

Outline

Atelocollagen neutral solutions are high purity collagen solutions prepared from bovine dermis and form a gel under physiological conditions. These products are pre-mixed with culture medium and ready to use for cell culture.

Applications

  • Culture-on-a-collagen-gel

    Culture on collagen gel

Feature

  • Atelocollagen neutral solutions are adjusted to neutral pH and form a gel when they are warmed to 37°C.

Type of collagen

Bovine hide derived atelocollagen

Cat. No. Product Packaging Price
MEN-02 Atelocollagen, Eagle’s MEM pH 7.4
Sterilized (Atelocollagen neutral solution)
This product is discontinued.
20 mL/bottle Visit distributor’s
web page
DME-02 Atelocollagen, DMEM Low Glucose pH 7.4
Sterilized (Atelocollagen neutral solution)
20 mL/bottle Visit distributor’s
web page
DME-02H Atelocollagen, DMEM High Glucose pH 7.4
Sterilized (Atelocollagen neutral solution)
20 mL/bottle Visit distributor’s
web page
RPM-02 Atelocollagen, RPMI 1640 pH 7.4
Sterilized (Atelocollagen neutral solution)
20 mL/bottle Visit distributor’s
web page

Storage: -20℃  For research use only.

Click here for frequently asked questions about collagen neutral solutions

Use case

References

  • 3D culture
  • Fabricating scaffold, miscellaneous
    • An in vitro three-dimensional co-culture system for ameloblastoma modelling
      Soo Leng Lee, Zainal Ariff Abdul Rahman, Hidetsugu Tsujigiwa, Mei Hamada, Kiyofumi Takabatake, Keisuke Nakano, Hitoshi Nagatsuka, Chong Huat Siar
      Sains Malaysiana 48(8)(2019): 1697–1706.
    • Enhancement of HGF-induced tubulogenesis by endothelial cell-derived GDNF.
      Nakasatomi M, Takahashi S, Sakairi T, Ikeuchi H, Kaneko Y, Hiromura K, Nojima Y, Maeshima A
      PLoS One. 2019 Mar 7;14(3):e0212991. PMID: 30845150
    • Osteogenic Potential of Mouse Periosteum-Derived Cells Sorted for CD90 In Vitro and In Vivo.
      Kim YK, Nakata H, Yamamoto M, Miyasaka M, Kasugai S, Kuroda S.
      Stem Cells Transl Med. 2016 Feb;5(2):227-34. PMID: 26718647
    • Investigation of VEGF and PDGF signals in vascular formation by 3D culture models using mouse ES cells.
      Hitomi Hosoe, Yuri Yamamoto, Yusuke Tanaka, Mami Kobayashi, Nana Ninagawa, Shigeko Torihashi.
      Stem Cell Discovery. 2012 2(2):70-77.
    • Age-related decline in label-retaining tubular cells: implication for reduced regenerative capacity after injury in the aging kidney.
      Miya M, Maeshima A, Mishima K, Sakurai N, Ikeuchi H, Kuroiwa T, Hiromura K, Nojima Y.
      Am J Physiol Renal Physiol. 2012 Mar 15;302(6):F694-702. PMID: 22169012
    • Enhancement of in vitro human tubulogenesis by endothelial cell-derived factors: implications for in vivo tubular regeneration after injury.
      Miya M, Maeshima A, Mishima K, Sakurai N, Ikeuchi H, Kuroiwa T, Hiromura K, Yokoo H, Nojima Y.
      Am J Physiol Renal Physiol. 2011 Aug;301(2):F387-95. PMID: 21561997
    • Activin A as a critical mediator of capillary formation: interaction with the fibroblast growth factor action.
      Hayashi Y, Maeshima K, Goto F, Kojima I.
      Endocr J. 2007 Apr;54(2):311-8. PMID:17384470
    • Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs.
      Akizuki T, Oda S, Komaki M, Tsuchioka H, Kawakatsu N, Kikuchi A, Yamato M, Okano T, Ishikawa I.
      J Periodontal Res. 2005 Jun;40(3):245-51.PMID: 15853971
    • Pkd1 regulates immortalized proliferation of renal tubular epithelial cells through p53 induction and JNK activation.
      Nishio S,Hatano M, Nagata M, Horie S, Koike T, Tokuhisa T, Mochizuki T.
      J Clin Invest. 2005 Apr;115(4):910-8. PMID: 15761494
    • Involvement of renal progenitor tubular cells in epithelial-to-mesenchymal transition in fibrotic rat kidneys.
      Yamashita S,Maeshima A, Nojima Y.
      J Am Soc Nephrol. 2005 Jul;16(7):2044-51. PMID: 15888566
    • Repair of articular cartilage defect by autologous transplantation of basic fibroblast growth factor gene-transduced chondrocyteswith adeno-associated virus vector.
      Yokoo N, Saito T, Uesugi M, Kobayashi N, Xin KQ, Okuda K, Mizukami H, Ozawa K, Koshino T.
      Arthritis Rheum. 2005 Jan;52(1):164-70. PMID: 15641065
    • Crucial role of activin a in tubulogenesis of endothelial cells induced by vascular endothelial growth factor.
      Maeshima K, Maeshima A, Hayashi Y, Kishi S, Kojima I.
      Endocrinology. 2004 Aug;145(8):3739-45. PMID: 15117880
    • JAM4 enhances hepatocyte growth factor-mediated branching and scattering of Madin-Darby canine kidney cells.
      Mori H, Hirabayashi S,Shirasawa M, Sugimura H, Hata Y.
      Genes Cells. 2004 Sep;9(9):811-9. PMID: 15330858
    • JAM4, a junctional cell adhesion molecule interacting with a tight junction protein, MAGI-1.
      Hirabayashi S, Tajima M, Yao I, Nishimura W,Mori H, Hata Y.
      Mol Cell Biol. 2003 Jun;23(12):4267-82. PMID: 12773569
    • Autocrine stimulation by osteopontin contributes to antiapoptotic signalling of melanocytes in dermal collagen.
      Geissinger E, Weisser C, Fischer P, Schartl M, Wellbrock C.
      Cancer Res. 2002 Aug 15;62(16):4820-8. PMID: 12183442
    • Gene marking in adeno-associated virus vector infected periosteum derived cells for cartilage repair.
      Kobayashi N, Koshino T, Uesugi M, Yokoo N, Xin KQ, Okuda K, Mizukami H, Ozawa K, Saito T.
      J Rheumatol. 2002 Oct;29(10):2176-80.PMID: 12375329
    • Role of HGF/c-met system in invasion and metastasis of oral squamous cell carcinoma cells in vitro and its clinical significance.
      Uchida D,Kawamata H, Omotehara F, Nakashiro Ki, Kimura-Yanagawa T, Hino S, Begum NM, Hoque MO, Yoshida H, Sato M, Fujimori T.
      Int J Cancer.2001 Aug 15;93(4):489-96. PMID: 11477552
    • Role of CD44 variant exon 6 in invasion of head and neck squamous cell carcinoma.
      Kanke M, Fujii M, Kameyama K, Kanzaki J, Tokumaru Y,Imanishi Y, Tomita T, Matsumura Y.
      Arch Otolaryngol Head Neck Surg. 2000 Oct;126(10):1217-23.PMID: 11031408
    • The miR-143/145 cluster induced by TGF-β1 suppresses Wilms' tumor 1 expression in cultured human podocytes.
      Tabei A, Sakairi T, Hamatani H, Ohishi Y, Watanabe M, Nakasatomi M, Ikeuchi H, Kaneko Y, Kopp JB, Hiromura K.
      Am J Physiol Renal Physiol. 2023 Jul 1;325(1):F121-F133. PMID: 37167274.
    • Distinguishing heat-treated dead cells from viable cells using frequency dependence of electrical impedance.
      Fujimaki M, Sei R, Yokoseki K, Nebuya S, Sakai R, Yoshida K, Ujihira M.
      Biomed Mater Eng. 2022;33(4):315-324. PMID: 35180102.
    • Cell fiber-based 3D tissue array for drug response assay.
      Kato-Negishi M, Sawayama J, Kawahara M, Takeuchi S.
      Sci Rep. 2022 May 12;12(1):7870. PMID: 35552465.
    • Comparison of cultured cell attachment on a temperature-responsive polymer, poly-L-lysine, and collagen using modeling curves and a thermal-controlled quartz crystal microbalance.
      Alsaleem AHA, Ito S, Naemura K, Muramatsu H
      J Biol Phys. 2021 Jun;47(2):117-129. PMID: 33893599
    • Long-Term Effect of Honeycomb β-Tricalcium Phosphate on Zygomatic Bone Regeneration in Rats.
      Nakagiri R, Watanabe S, Takabatake K, Tsujigiwa H, Watanabe T, Matsumoto H, Kimata Y.
      Materials (Basel). 2020 Nov 26;13(23):5374. PMID: 33256248.
    • Cell-laden microfibers fabricated using μl cell-suspension.
      Nie M, Nagata S, Aoyagi H, Itou A, Shima A, Takeuchi S.
      Biofabrication. 2020 Aug 5;12(4):045021. PMID: 32299072.
    • Microfluidic Device for the Analysis of Angiogenic Sprouting under Bidirectional Biochemical Gradients.
      Nishimura K, Nie M, Miura S, Takeuchi S.
      Micromachines (Basel). 2020 Nov 27;11(12):E1049. PMID: 33261134.
    • Effect of Honeycomb β-TCP Geometrical Structure on Bone Tissue Regeneration in
      Watanabe T, Takabatake K, Tsujigiwa H, Watanabe S, Nakagiri R, Nakano K, Nagatsuka H, Kimata Y.
      Skull Defect. Materials (Basel). 2020 Oct 25;13(21):4761. PMID: 33113818.
    • Rod-Shaped Neural Units for Aligned 3D Neural Network Connection.
      Kato-Negishi M, Onoe H, Ito A, Takeuchi S.
      Adv Healthc Mater. 2017 Aug;6(15). PMID: 28429415
    • Parkin promotes proteasomal degradation of synaptotagmin IV by accelerating polyubiquitination.
      Kabayama H, Tokushige N, Takeuchi M, Kabayama M, Fukuda M, Mikoshiba K.
      Mol Cell Neurosci. 2017 Apr;80:89-99.PMID: 28254618
    • ASF-4-1 fibroblast-rich culture increases chemoresistance and mTOR expression of pancreatic cancer BxPC-3 cells at the invasive front in vitro, and promotes tumor growth and invasion in vivo.
      Fujiwara M, Kanayama K, Hirokawa YS, Shiraishi T.
      Oncol Lett. 2016 Apr;11(4):2773-2779. PMID: 27073551
    • Long-term gene therapy with Del1 fragment using nonviral vectors in mice with explanted tumors.
      Kitano H, Mamiya A, Ishikawa T, Egoshi K, Kokubun S, Hidai C.
      Onco Targets Ther. 2016 Jan 25;9:503-16. PMID: 26889088
    • Differentiation Induction of Mouse Neural Stem Cells in Hydrogel Tubular Microenvironments with Controlled Tube Dimensions.
      Onoe H, Kato-Negishi M, Itou A, Takeuchi S.
      Adv Healthc Mater. 2016 May;5(9):1104-11. PMID: 26919482
    • Smooth muscle-like tissue constructs with circumferentially oriented cells formed by the cell fiber technology.
      Hsiao AY, Okitsu T, Onoe H, Kiyosawa M, Teramae H, Iwanaga S, Kazama T, Matsumoto T, Takeuchi S.
      PLoS One. 2015 Mar 3;10(3):e0119010. PMID: 25734774
    • Neural stem/progenitor cell-laden microfibers promote transplant survival in a mouse transected spinal cord injury model.
      Sugai K, Nishimura S, Kato-Negishi M, Onoe H, Iwanaga S, Toyama Y, Matsumoto M, Takeuchi S, Okano H, Nakamura M.
      J Neurosci Res. 2015 Dec;93(12):1826-38. PMID: 26301451
    • Hepatocyte Selection Medium.
      Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto and Makoto Sueishi.
      Pluripotent Stem Cell Biology - Advances in Mechanisms, Methods and Models. Prof. Craig Atwood (Ed.), ISBN: 978-953-51-1590-8, InTech, DOI: 10.5772/58394.
    • Preparation of stripe-patterned heterogeneous hydrogel sheets using microfluidic devices for high-density coculture of hepatocytes and fibroblasts.
      Kobayashi A, Yamakoshi K, Yajima Y, Utoh R, Yamada M, Seki M.
      J Biosci Bioeng. 2013 Dec;116(6):761-7. PMID: 23845912
    • Metre-long cell-laden microfibres exhibit tissue morphologies and functions.
      Onoe H, Okitsu T, Itou A, Kato-Negishi M, Gojo R, Kiriya D, Sato K, Miura S, Iwanaga S, Kuribayashi-Shigetomi K, Matsunaga YT, Shimoyama Y, Takeuchi S
      Nat Mater. 2013 Jun;12(6):584-90. PMID: 23542870
    • Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions.
      Yamada M, Utoh R, Ohashi K, Tatsumi K, Yamato M, Okano T, Seki M.
      Biomaterials. 2012 Nov;33(33):8304-15. PMID: 22906609
    • Molding cell beads for rapid construction of macroscopic 3D tissue architecture.
      Matsunaga YT, Morimoto Y, Takeuchi S.
      Adv Mater. 2011 Mar 25;23(12):H90-4. PMID:21360782
    • Evaluation by bone scintigraphy of osteogenic activity of commercial bioceramics (porous beta-TCP and HAp particles) subcutaneously implanted in rats.Nakayama H, Kawase T, Kogami H, Okuda K, Inoue H, Oda T, Hayama K, Tsuchimochi M, Wolff LF.
      J Biomater Appl. 2010 May;24(8):751-68. PMID: 19726531
    • A dynamic microarray device for paired bead-based analysis.
      Teshima T, Ishihara H, Iwai K, Adachi A, Takeuchi S.
      Lab Chip. 2010 Sep 21;10(18):2443-8. PMID: 20697655
    • The effect of gradually graded shear stress on the morphological integrity of a huvec-seeded compliant small-diameter vascular graft.
      Inoguchi H, Tanaka T, Maehara Y, Matsuda T.
      Biomaterials. 2007 Jan;28(3):486-95. PMID: 17034847
    • Delivery of a growth factor fusion protein having collagen-binding activity to wound tissues.
      Ishikawa T, Terai H, Yamamoto T, Harada K, Kitajima T.
      Artif Organs. 2003 Feb;27(2):147-54. PMID: 12580771
    • Development of composite cultured oral mucosa utilizing collagen sponge matrix and contracted collagen gel: a preliminary study for clinical applications.
      Moriyama T, Asahina I, Ishii M, Oda M, Ishii Y, Enomoto S.
      Tissue Eng. 2001 Aug;7(4):415-27. PMID: 11506731
    • Cytotoxicity of reactive oxygen species and related agents toward undifferentiated and differentiated rat phenochromocytoma PC12 cells.
      Sasaki N, Baba N, Matsuo M.
      Biol Pharm Bull. 2001 May;24(5):515-519. PMID: 11379772
    • Inhibitory effects of human serum on human fetal skin fibroblast migration: migration-inhibitory activity and substances in serum, and its age-related changes.
      Kondo H, Yonezawa Y, Ito H.
      In Vitro Cell Dev Biol Anim. 2000 Apr;36(4):256-61. PMID: 10852351