Collagen Acidic/Neutral Solutions Q&A | KOKEN CO., LTD. | KOKEN CO., LTD.

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Collagen Acidic Solutions/Neutral Solutions

What are the differences between I-PC and I-AC?

I-PC is protease solubilised atelocollagen whose immunogenicity is reduced while maintaining the functions of collagen. I-PC is suitable both for gel formation and for collagen coating because of its relatively low viscosity. On the other hand, I-AC is acid-soluble collagen which retains telopeptides. Its viscosity, transparency and rigidity are higher than those of I-PC.

What is atelocollagen?

Telopeptide is a non-helical structure at both ends of the collagen molecule and is thought to be involved in intermolecular bonding and allergenic reaction due to antigenicity. Removing the telopeptides from collagen by protease treatment produces atelocollagen whose characteristics are superior purity and stability. We have produced atelocollagen-based medical devices for more than 30 years.

What types of collagen are contained in Collagen acidic solutions and Collagen neutral solutions?

The source of collagen is bovine dermis and therefore both products contain approximately 95% type I collagen and roughly 5% type III and type V collagen.

What is the difference between collagen acidic solutions and collagen neutral solutions?

Collagen is highly stable in acidic conditions and is appropriate for long-term storage. In contrast, collagen forms a gel under physiological conditions and thus must be neutralized and mixed with medium. This is why we have prepared collagen neutral solution which readily forms a gel upon warming. The collagen contained in collagen neutral solution is atelocollagen.

How can I prepare collagen coatings/collagen gels?

Please visit our distributors’ page to download an instruction manual

How can I prevent cells from sinking during collagen gel formation?

When a low concentration of collagen is used, the viscosity is low and the gelling time is longer. We recommend using 5 mg/mL Atelocollagen or Native collagen acidic solution (Cat No. IPC-50 or IAC-50).

How can I harvest cells from collagen gel?

Add collagenase to final concentration of 0.1% and incubate at 37°C for approximately an hour.

Is it possible to prepare sections from collagen gel?

Similar to tissue samples, collagen gel can be fixed and/or embedded in paraffin/OCT compound.

Is there a high concentration collagen solution that is ideal for cell transplantation?

We can provide a collagen solution equivalent to that used in manufacturing our atelocollagen-based medical devices as a made-to-order research product. Alternatively, a high concentration collagen solution can be prepared from Atelocollagen powder (Cat No. CLP-01).

What are the differences between soluble basement membrane extracts and collagen solution?

Our collagen solution does not contain cell-derived physiologically active compounds, nucleic acids, matrix metalloproteinase (MMP), etc., which could affect the results of your experiment. Unlike soluble basement membrane extracts, we confirm the lot-to-lot consistency of our collagen products.

 

Information on this page is based on published articles and in-house data.