Atelocollagen Permeable Membrane Q&A | KOKEN CO., LTD. | KOKEN CO., LTD.

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Atelocollagen, Permeable Membrane

How can I seed cells on Atelocollagen membrane?

Please visit our distributors’ page to download an instruction manual

Why do I have to neutralize Atelocollagen membrane before use?

The membrane is made from atelocollagen acidic solution and neutralization is necessary prior to cell culture.

How can I harvest cells from Atelocollagen membrane?

Use a cell scraper to harvest cells. We have confirmed that the membrane will not degrade upon treatment with lysis buffers contained in nucleic acid extraction kits.

Is it possible to prepare sections from Atelocollagen membrane?

Similar to tissue samples, Atelocollagen membrane can be fixed and/or embedded in paraffin/OCT compound.

How can I observe cells on Atelocollagen membrane?

Cells can be observed using a phase-contrast microscope. We recommend labelling cells beforehand so that you can easily identify cells seeded on each side of the membrane.

What are the differences between MEN-01 and CM-6/CM-24?

The same membrane is used for all three products but the shapes and sizes of the plastic frames holding the membrane are different. We also have Atelocollagen membrane without a plastic frame.

What are the differences between cell culture inserts and Atelocollagen membrane?

Usually a cell culture insert is made from synthetic resin while Atelocollagen membrane is made from 100% collagen. The membrane does not have definitive pores and cells cannot penetrate to the other side of the membrane, yet the membrane is permeable to small molecules. This is why cells can be harvested separately from each side of the membrane following co-culture. The membrane is biodegradable and suitable for cell transplantation.

Is it possible to obtain custom-made Atelocollagen membrane that is different in size and thickness from the off-the-shelf product?

Please contact us as we may be able to produce a custom-made product upon your request. You can also cut the membrane into smaller pieces with a sharp knife such as a surgical scalpel.

Information on this page is based on published articles and in-house data.