We have formed tight junctions between adjacent renal tubular epithelial cells, created epidermal models using keratinocytes, and induced osteoblast osteogenic differentiation.

Please visit our distributors’ page to download for the instruction manual.

The equilibration with medium generates a suitable environment for cell culture.

The recovery is performed with trypsin solution. We have confirmed that adding trypsin solution to the membrane does not dissolve it.

In a similar way to tissue samples, frozen embedding and sectioning can be performed after fixation with formalin, etc.

You can see cultured cells using a low-magnification phase-contrast microscope; observation of individual cells at high magnification is difficult. For detailed observation, it is recommended to perform immunostaining, etc. and histological assessment after culture completion.

Although the membrane portion of both types of products is made from atelocollagen, FibColl® Atelocollagen Inserts have a high substance permeability that allows the permeation of molecules even over 600 kDa, produced with a unique fibril formation method of  atelocollagen. Therefore, although conventional atelocollagen permeable membranes show high transparency, they have a limited permeation molecular weight.

Our products are made of 100% atelocollagen. Thus, the pores of FibColl® Atelocollagen Inserts are not clogged by coatings while its membranes retain the fibrous structure of atelocollagen. Moreover, even though FibColl® is permeable to certain substances, cells cannot pass through it. Therefore, it is possible to collect cells from each culture surfaces after co-culture. In addition, FibColl® is biodegradable, making it suitable for cell transplantation.

If the transplantation site can be incised, transplantation of an atelocollagen sponge or membrane-containing cells is applicable.

If the incision is less preferable, it is possible to mix cells with a high-concentration of atelocollagen solution and administer it locally so that the collagen forms a gel and cells can be transplanted to the injection site.

We can produce a customizable product, so please contact us first. For smaller items than the off-the-shelf product, it is possible to use a sharp knife such as a scalpel.