Several articles have shown dose-dependent effects but there are no reports of problems encountered by using concentrations of siRNA/miRNA higher than the recommended concentration.

We recommend preparing the mixture at the time of use. It is possible to prepare the mix on a small scale if you need a small quantity. Please ask us for details.

The following table lists some tissue-specific applications and the recommended doses of the solution.

It is recommended to prepare the solution such that the amount of nucleic acid per administration site is the same as our recommendation (0.5– 1 nmol/site).

 Although the recommended dose of AteloGene^® is specifically for mice, it has been used for rats and rabbits by adjusting the dose appropriately for the tissue size of the animal.

It has been locally injected in models of subcutaneous tumors, diabetes, and peripheral neuropathy.

Various nucleic acids such as siRNA, shRNA, miRNA, miRNA inhibitor, locked nucleic acid, antisense oligos, single-stranded oligonucleotides, and plasmid DNA have been used with AteloGene Local Use. The main component of AteloGene, atelocollagen, is positively charged and therefore, binds anionic nucleic acids to form complexes.

AteloGene® Local Use/Local Use Quick Gelation has been used with plasmid vectors up to 10 kbp in length.

Approximately a week following local administration.

It will degrade after approximately a week, depending on the injection site, after local administration.

We recommend covering the tumour with AteloGene^® to avoid damage from injection. However, there are many articles describing the use of intra-tumoural injections.

AteloGene^® is designed for in vivo experiments only. Please use AteloGene^® for your in vivo experiments following in vitro evaluation of siRNA/miRNA.