AteloGene® Local Use for in vivo transfection | Koken Co., Ltd.

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AteloGene® Local Use

Outline

This product will be discontinued when the stock is run out due to introduction of a successor product AteloGene® Local Use “Quick Gelation”.

This in vivo transfection kit is designed for delivering siRNA/miRNA into animal tissues, mouse in particular, to introduce the siRNA/miRNA into cells by local administrations.
Atelocollagen is positively charged and can thus electrostatically form complexes with nucleic acids when they are mixed together at appropriate concentrations and ratios. The complexes protect nucleic acids from being degraded by nucleases in in vivo.

What is Atelocollagen?

Applications

Local administration of nucleic acids

Features

  • Prolonged in vivo RNAi effect because atelocollagen, the main component of AteloGene® products, form complexes with siRNA/miRNA
  • Since AteloGene® products are made from atelocollagen, it is nontoxic.
  • AteloGene® Local Use gelates in the body and siRNA/miRNA are slowly released from the gel at the site of administration.

Kit contents

① AteloGene® Local Use prefilled syringe
　(Including losses during the preparation steps)		 600 μL × 2 syringes
②10×siRNA buffer
(It can be used for miRNA as well)		 3 mL × 1 bottle
③Sterilized water 3 mL × 1 bottle
④2 mL microtube 2 tubes
⑤Disposable Syringe 2 tubes
⑥18G needle 4 needles
⑦26G needle 2 tubes
⑧Instruction manual 1 leaflet

 

Devices and reagents required other than those in the kit

  • siRNA/miRNA (PAGE- or HPLC-purified grade is recommended.)
  • Container for preparation of siRNA/miRNA solution (sterilized, RNase-free)
  • Cooling device (crushed ice, cold block, etc.)
  • Tube rotator (that can tumble and agitate such as TAITEC RT-5, Bibby scientific SB3, etc.)
  • Pipetter and tips (sterilized, RNase-free)
  • High-speed refrigerated centrifuge
  • Anesthetic
Cat. No. Product Package Price
#1392
for Cosmo Bio customers

#1394
for REPROCELL customers

 AteloGene® Local Use 1 kit
(10 administrations) *		 Visit distributor’s
web page

*It can be used more than 10 administrations depending on target tissue because one administration is assumed to be 200μl.

Storage: 2-10°C  For research use only.
Expiration date: 5 years from manufacturing date printed on the box.

Click here for frequently asked questions about AteloGene® Local Use

Experimental example

Local siRNA administration to mouse xenograft model with AteloGene® Local Use

After local administration of a luciferase siRNA (Luc siRNA) & AteloGene® mixture to subcutaneous tumours expressing luciferase, luciferase expression was markedly reduced when compared to the control.

Use case

References

    • For reference list, please visit AteloGene Local Use "Quick Gelation" product page.