Microcarrier for cell culture Atelocollagen Microspheres | Koken Co., Ltd.

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Atelocollagen Microspheres

Outline

Atelocollagen microspheres are microbeads-like atelocollagen scaffold for cell culture. This carrier is useful for culturing adhesive cells such as fibroblasts, epithelial cells and osteoblasts due to its ability to maintain cell functions.

Applications

  • Mass-cell-culture-with-Collagen-Microspheres

    Mass-cell culture

Features

  • Collagen microspheres create an in vitro-like environment because they are produced exclusively from fibril-forming type I atelocollagen.

  • Cells are easily harvested by collagenase treatment.

Types of collagen

Bovine hide derived atelocollagen

Cat. No. Product Packaging Price
MIC-00

Atelocollagen Microspheres  Sterilized
Particle number: Approx. 3 million particles/15mL
Surface area: Approx. 3,800 cm²/15mL
Size: φ100-400μm

 15 mL/bottle*
*25 mL including PBS		 Visit distributor’s
web page

Storage: 2-10℃ (Do not freeze)  For research use only.

Click here for frequently asked questions about Atelocollagen microspheres

Use case

References

  • Bone, Tooth, Cartilage
  • Miscellaneous
    • microRNA-875-5p plays critical role for mesenchymal condensation in epithelial-mesenchymal interaction during tooth development.
      Funada K, Yoshizaki K, MIyazaki K, Han X, Yuta T, Tian T, Mizuta K, Fu Y, Iwamoto T, Yamada A, Takahashi I, Fukumoto S.
      Sci Rep. 2020 Mar 18;10(1):4918. PMID: 32188878
    • Strategy for the Generation of Engineered Bone Constructs Based on Umbilical Cord Mesenchymal Stromal Cells Expanded with Human Platelet Lysate.
      Silva-Cote I, Cruz-Barrera M, Cañas-Arboleda M, Correa-Araujo L, Méndez L, Jagielska J, Camacho B, Salguero G.
      Stem Cells Int. 2019 Dec 1;2019:7198215. PMID: 31885622
    • Efficacy of bone regeneration using collagen microspheres as scaffold.
      Shunsuke Baba, Naoyuki Matsumoto, Yuki Kaneshita, Kaoru lnami, Hidetoshi Morikuni and Takayoshi Kawazoe.
      J Osaka Dent Univ. 2008. 42(1), 9-15.
    • Mandibular reconstruction using a combination graft of rhBMP-2 with bone marrow cells expanded in vitro.
      Seto I, Marukawa E, Asahina I.
      Plast Reconstr Surg. 2006 Mar;117(3):902-908. PMID: 16525283
    • Propagation of human nasal chondrocytes in microcarrier spinner culture.
      Shikani AH, Fink DJ, Sohrabi A, Phan P, Polotsky A, Hungerford DS, Frondoza CG.
      Am J Rhinol. 2004 Mar-Apr;18(2):105-12. PMID: 15152876
    • The role of osteopontin on calcium oxalate crystal formation.
      Konya E, Umekawa T, Iguchi M, Kurita T.
      Eur Urol. 2003 May;43(5):564-71. PMID: 12706004
    • Collagen microcarrier spinner culture promotes osteoblast proliferation and synthesis of matrix proteins.
      Overstreet M, Sohrabi A, Polotsky A, Hungerford DS, Frondoza CG.
      In Vitro Cell Dev Biol Anim. 2003 May-Jun;39(5-6):228-34. PMID: 14613330
    • The effect of osteopontin immobilized collagen granules in the seed crystal method.
      Umekawa T, Iguchi M, Kurita T.
      Urol Res. 2001 Aug;29(4):282-6. PMID: 11585285
    • Beta-1 integrin expression by human nasal chondrocytes in microcarrier spinner culture.
      Bouchet BY, Colón M, Polotsky A, Shikani AH, Hungerford DS, Frondoza CG.
      J Biomed Mater Res. 2000 Dec 15;52(4):716-24. PMID: 11033555
    • Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture.
      Frondoza C, Sohrabi A, Hungerford D.
      Biomaterials. 1996 May;17(9):879-88.　PMID: 8718933
    • Comparative histological studies of bone and cartilage formations induced by various BMP-carrier composites.:Comparative histological studies of bone and cartilage formations induced by various BMP-carrier composites.
      Liliana Missana, Noriyuki Nagai and Yoshinori Kuboki
      Japanese Association for Oral Biology 36(1), 9-19, 1994
    • Cross-linked collagen gel spheres as a useful carrier for cell culture of MC 3T3-E1 clonal osteogenic cells.
      Morimichi Mizuno, Tomoyo Kasagi, Yoshinori Kuboki.
      Japanese J. Oral Biol. 1988 30(6):855-8.
    • Atelocollagen supports three-dimensional culture of human induced pluripotent stem cells.
      Nakashima Y, Tsukahara M.
      Mol Ther Methods Clin Dev. 2024 Jul 20;32(3):101302. PMID: 39185274.
    • A novel cell source for therapy of knee osteoarthritis using atelocollagen microsphere-adhered adipose-derived stem cells: Impact of synovial fluid exposure on cell activity.
      Sakamoto T, Fuku A, Horie T, Kitajima H, Nakamura Y, Tanida I, Sunami H, Hirata H, Tachi Y, Iida Y, Yamada S, Yamamoto N, Shimizu Y, Ishigaki Y, Ichiseki T, Kaneuji A, Osawa S, Kawahara N.
      Regen Ther. 2024 Apr 23;27:408-418. PMID: 38694445.
    • Accelerated vascularization of a novel collagen hydrogel dermal template.
      Weisel A, Cohen R, Spector JA, Sapir-Lekhovitser Y.
      J Tissue Eng Regen Med. 2022 Dec;16(12):1173-1183. PMID: 36219532.
    • Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
      Mashiko T, Kanayama K, Saito N, Shirado T, Asahi R, Mori M, Yoshimura K.
      Cells. 2021 Mar 4;10(3):560. PMID: 33806638
    • Type I collagen can function as a reservoir of basic fibroblast growth factor.
      Kanematsu A, Marui A, Yamamoto S, Ozeki M, Hirano Y, Yamamoto M, Ogawa O, Komeda M, Tabata Y.
      J Control Release. 2004 Sep 30;99(2):281-92. PMID: 15380637
    • Expression of vascular endothelial growth factor promotes colonization, vascularization, and growth of transplanted hepatic t issues in the mouse.
      Ajioka I, Akaike T, Watanabe Y.
      Hepatology. 1999 Feb;29(2):396-402. PMID:9918915
    • Correlation between cell-adherent activity and surface structure in Porphyromonas gingivalis.
      Watanabe K, Yamaji Y, Umemoto T.
      Oral Microbiol Immunol. 1992 Dec;7(6):357-63. PMID: 1363734