3D Honeycomb Boosted for High-density 3D culture | Koken Co., Ltd.

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3D Honeycomb Boosted

Outline

The 3D Honeycomb Boosted is a carrier for high-density three-dimensional culture (3D culture) made by processing atelocollagen into a spongy scaffold with honeycomb-like through-holes. With a size of Φ5 mm x height 5 mm, which fits in a 96-well plate, it is possible to cultivate 106 cells on a scale approaching that of a 35 mm dish.

Applications

  • 3D-culture-in-3D-Honeycom-Boosted

    3D culture

  •  Cell transplantation

Features

  • The honeycomb structure facilitates the access of nutrients to the cultured cells and the removal of waste products from the sponge, allowing cells to proliferate within the sponge.

  • It has high resilience to return to its original shape even after compression.

  • Cells are easily harvested by collagenase treatment.

  • It is biodegradable and suitable for cell transplantation.
  • It is immersed in PBS, eliminating the need for static electricity countermeasures and defoaming.

Type of collagen

Bovine hide derived atelocollagen

  • SEM image of 3D Honeycomb Boosted

  • Overall view after culturing GFP-expressing HepG2 cells for 7 days (Fluorescence microscopy image)

  • Sectional view after culturing GFP-expressing HepG2 cells for 7 days (Fluorescence microscopy image)

Cat. No. Product Packaging Price
3D-HCB 3D Honeycomb Boosted  Sterilized
Φ5mm×5mm 
Pore size: 100-300μm		 25  pieces/bottle Visit distributor’s
web page

Storage: 2-10℃  For research use only.

Click here for frequently asked questions about 3D Honeycomb Boosted

Use case

References

  • 3D cell culture
  • Cell transplantation