Collagen Sponge for 35 mm Culture Dish has been used for in vitro tissue models and calcification studies by seeding and culturing fibroblasts and osteoblasts on the sponges.

This sponge is made from native collagen and retains the telopeptides, which have antigenicity. We recommend using MIGHTY or Honeycomb sponge for customers wishing to perform cell transplantation.

The thickness is 1 to 2 mm but may vary within the same piece.

There is a thin layer of collagen at the surface of this sponge so cells remain on the sponge surface after cell seeding. Following cell culture for approximately a week, the cells begin to penetrate the inside of the sponge.

Cells were retrieved by collagenase treatment.