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Seven types of collagen, seven papers
Applications in cancer research
From coculture with cancer-associated fibroblasts to orthotopic transplantation models of patient-derived cancer
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Cancer-associated fibroblasts (CAFs), which constitute the cellular component of the tumor microenvironment, show a high correlation with the onset of cancer and are garnering much attention in current research. As CAFs are known to remodel the extracellular matrix within the cancer microenvironment, cancer research and collagen are inseparable.
Here, we present, with accompanying illustrations, examples of how seven types of collagen products were used for the ex vivo culture of patient-derived cancer tissues and the administration of dsRNAi with antitumor effects to orthotopic transplant models.
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<< Pancreatic ductal adenocarcinoma (PDAC) and CAF progenitor cells cocultured in a gel >>
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Collagen Lattice Model, Populated with Heterogeneous Cancer-Associated Fibroblasts, Facilitates Advanced Reconstruction of Pancreatic Cancer Microenvironment.
Song X, et al. Int J Mol Sci. 2024 Mar 27;25(7):3740. PMID: 38612551.
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PDAC and CAF progenitor cells were cocultured in a gel made from a native collagen (I-AC) solution to evaluate CAF differentiation. After coculture, the gel was subcutaneously implanted into mice to evaluate its tumor-forming ability.
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<< Cervical cancer tissue from patients cultured ex vivo on a sponge >>
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9-oxo-ODAs suppresses the proliferation of human cervical cancer cells through the inhibition of CDKs and HPV oncoproteins.
Mogi K, et al. Sci Rep. 2023 Nov 6;13(1):19208. PMID: 37932321.
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Cervical cancer tissue fragments were cultured ex vivo on Honeycomb disc 96, and 9-oxo-ODAs were added to evaluate the antitumor effect.
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<< Double-sided culture of osteosarcoma spheroids and vascular endothelial cells >>
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Pigment Epithelium Derived Factor Is Involved in the Late Phase of Osteosarcoma Metastasis by Increasing Extravasation and Cell-Cell Adhesion.
Kuriyama S, et al. Front Oncol. 2022 Jan 31;12:818182. PMID: 35174090.
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Vascular endothelial cells were seeded on the bottom side of a permeable collagen membrane, and osteosarcoma cell spheroids were seeded on the upper surface and cocultured. The effect of the pigment epithelium-derived factor (PEDF) on the vascular extravasation was evaluated.
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<< Melanoma cells cultured in epidermal growth factor (EGF) concentration gradient gels >>
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RSK/GSK3-mediated phosphorylation of FilGAP regulates chemotactic cancer invasion.
Tsutsumi K, Ohta Y. PNAS Nexus. 2024 Feb 9;3(2):pgae071. PMID: 38426123.
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Melanoma cells seeded in glass-bottom dishes were covered with atelocollagen (I-PC) solution, and gels with an EGF concentration gradient were prepared to evaluate chemotactic infiltration.
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<< 3D culture of breast cancer cell spheroids in a gel >>
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Metabolic characterization of aggressive breast cancer cells exhibiting invasive phenotype: impact of non-cytotoxic doses of 2-DG on diminishing invasiveness.
Fujita M, et al. BMC Cancer. 2020 Sep 29;20(1):929. PMID: 32993545.
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Breast cancer cell spheroids were cultured in 3D-Ready atelocollagen gels, and the correlation between the mitochondrial membrane potential (∆ψm) and invasion was evaluated.
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<< Local administration of siRNA in a subcutaneous tumor model of liver cancer >>
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PRPF8 increases the aggressiveness of hepatocellular carcinoma by regulating FAK/AKT pathway via fibronectin 1 splicing.
López-Cánovas JL, et al. Exp Mol Med. 2023 Jan;55(1):132-142. PMID: 36609600.
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Mouse models of subcutaneous hepatocellular carcinoma tumors were generated, and siRNA was administered to the tumor periphery using AteloGene® Local Use “Quick Gelation” (AG for loacal administration) to assess the antitumor effect.
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<< Systemic administration of dsRNAi in an orthotopic implantation model of patient-derived undifferentiated thyroid cancer cells >>
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dsRNAi-mediated silencing of PIAS2beta specifically kills anaplastic carcinomas by mitotic catastrophe.
Rodrigues JS, et al. Nat Commun. 2024 May 14;15(1):3736. PMID: 38744818.
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Orthotopic implantation mouse models of patient-derived anaplastic thyroid carcinoma cells were created to determine the antitumor effects of dsRNAi administered via the tail vein using AteloGene® Systemic Use (AG for systemic administration).
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<< Exhibition Information >>
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We plan to participate in the annual meeting of the Cancer Association in September. Please visit us at the venue to see our atelocollagen products.
83rd Annual Meeting of the Japanese Cancer Association
Date: September 19 (Thursday) to September 21 (Saturday)
Venue: Fukuoka Marine Messe
Booth: 12 |
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