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コラーゲン酸性溶液I-PC、I-AC
Atelocollagen/Native Collagen Acidic Solutions

概要

本製品はウシ皮由来の高純度コラーゲン酸性溶液であり、三次元培養(3D培養)や共培養用のスキャフォールド(足場材料)としてのコラーゲンゲルの作製、ディッシュやプレート等へのコーティングに適しています。I-PC(アテロコラーゲン)およびI-AC(ネイティブコラーゲン)の2種類があり、アテロコラーゲンはネイティブコラーゲンの分子両末端に存在するテロペプチドが、プロテアーゼによって切断されています。

アテロコラーゲンとは

 

用途

  • コラーゲンコーティング

  • 三次元培養(3D培養)

  • コラーゲンゲル上培養

  • コラーゲンゲル共培養

特長

  • I-PC(アテロコラーゲン)、I-AC(ネイティブコラーゲン)はいずれも中性にすると生理的条件下でゲル化(線維化)します。
  • 3mg/mLと5mg/mLの濃度が選べ、それぞれコーティング、三次元培養(3D培養)や共培養用のスキャフォールド(足場材料)としてのコラーゲンゲル作製に適しています。
  • 可溶性基底膜調製品とは異なり、細胞由来の生理活性物質や核酸、MMP等を含まないため、実験結果を明確に評価できます。
 

コラーゲンの種類

コラーゲン酸性溶液I-PC :ウシ皮由来アテロコラーゲン
コラーゲン酸性溶液I-AC :ウシ皮由来ネイティブコラーゲン

製品の一時的な容器変更のお知らせ
容器の世界的な供給不足のため、別表の通り代替容器にてお届けしております。代替容器についても供給が不安定なため、別の代替容器へ変更することがございます。容器の供給が安定になり次第、従来の容器に戻す予定です。 容器の一時的な変更に伴う型番、仕様、価格、発注方法に変更はございません。

製品番号 製品 包装 価格
IPC-30 コラーゲン酸性溶液I-PC 3mg/mL pH3.0 無菌
Atelocollagen Acidic Solution
50mL/本 ¥9,800
(税込¥10,780)
IPC-50 コラーゲン酸性溶液I-PC 5mg/mL pH3.0 無菌
Atelocollagen Acidic Solution
50mL/本 ¥14,500
(税込¥15,950)
IAC-30 コラーゲン酸性溶液I-AC 3mg/mL pH3.0 無菌
Native Collagen Acidic Solution
50mL/本 ¥16,500
(税込¥18,150)
IAC-50 コラーゲン酸性溶液I-AC 5mg/mL pH3.0 無菌
Native Collagen Acidic Solution
50mL/本 ¥24,500
(税込¥26,950)

保管方法:冷蔵(2〜10℃) 本品は研究用試薬です。
代理店への仕切り価格は、家田化学薬品株式会社(03-3816-1871)へご確認ください。

参考文献

  • I-PC
    三次元培養
    (3D培養)
  • I-PC
    共培養
  • I-PC
    培養器材
    コーティング
  • I-PC
    スキャフォールド作製/コーティング
  • I-PC
    ゲル上培養・その他
  • I-AC
    三次元培養
    (3D培養)
  • I-AC
    共培養・
    力学刺激培養
  • I-AC
    培養器材
    コーティング
  • I-AC
    スキャフォールド作製/コーティング
  • I-AC
    ゲル上培養・その他
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      初代ヒト線維芽細胞をコラーゲンゲル内三次元培養。
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      歯根膜幹細胞スフェロイドをコラーゲンゲル内三次元培養。
    • Inhibitory effects of miRNAs in astrocytes on C6 glioma progression via connexin 43.
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    • Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.
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      尿細管上皮細胞をコラーゲンゲル内三次元培養。
    • Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro.
      Hosoya T, Tsuchiya I, Ohta T, Benhanifia M, Kumazawa S.
      Molecules. 2021 Oct 28;26(21):6510. PMID: 34770923.
      血管内皮細胞のサンドイッチ培養。
    • HDGF Enhances VEGF‑dependent Angiogenesis and FGF‑2 Is a VEGF‑independent Angiogenic Factor in Non‑small Cell Lung Cancer
      Eguchi R, Wakabayashi I.
      Oncol Rep. 2020 Apr 9. Online ahead of print. PMID: 32319650
      血管内皮細胞のコラーゲンゲル内三次元培養およびコラーゲンゲルサンドイッチ培養。また、肺がん細胞のコラーゲンゲル三次元培養。
    • Antiangiogenic Activity of Flavonols in Chorioallantoic Membrane (CAM) Assay.
      Naoki Okamura, Toshiro Ohta, Kazuhiro Kunimasa, Yoshihiro Uto, Shigenori Kumazawa.
      Food Science and Technology Research, 26 (6), 891_896, 2020
      血管内皮細胞のコラーゲンゲル内三次元培養。
    • Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity.
      Otani T, Nguyen TP, Tokuda S, Sugihara K, Sugawara T, Furuse K, Miura T, Ebnet K, Furuse M.
      J Cell Biol. 2019;218(10):3372-3396. PMID: 31467165
      尿細管上皮細胞をインサート上でコラーゲンゲル内培養。
    • Component Analysis and Antiangiogenic Activity of Thailand Stingless Bee Propolis. Ishizu, E., Honda, S., Ohta, T., Vongsak, B., & Kumazawa, S. (2019).
      Makara Journal Of Technology, 23(2), 77-82.
      血管内皮細胞のサンドイッチ培養。
    • Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver.
      Tanimizu N.
      Methods Mol Biol. 2019;1905:9-17. PMID: 30536086
      カバースリップをコラーゲン+マトリゲルでコーティングし、肝細胞を播種した後、マトリゲルを重層して培養。
    • FilGAP regulates distinct stages of epithelial tubulogenesis.
      Zuinen T, Tsutsumi K, Ohta Y.
      Biochem Biophys Res Commun. 2019 Jun 30;514(3):742-749. PMID: 31078260
      腎臓尿細管上皮細胞をコラーゲンゲル内三次元培養。
    • Rod-Shaped Neural Units for Aligned 3D Neural Network Connection.
      Kato-Negishi M, Onoe H, Ito A, Takeuchi S.
      Adv Healthc Mater. 2017 Aug;6(15). PMID: 28429415
      初代大脳皮質細胞+コラーゲンが内側、アルギン酸が外側の二層構造細胞ファイバーを作製し、コラーゲンゲル中でタイムラプスイメージング。
    • Sustained inflammation after pericyte depletion induces irreversible blood-retina barrier breakdown.
      Ogura S, Kurata K, Hattori Y, Takase H, Ishiguro-Oonuma T, Hwang Y, Ahn S, Park I, Ikeda W, Kusuhara S, Fukushima Y, Nara H, Sakai H, Fujiwara T, Matsushita J, Ema M, Hirashima M, Minami T, Shibuya M, Takakura N, Kim P, Miyata T, Ogura Y, Uemura A.
      JCI Insight. 2017 Feb 9;2(3):e90905. PMID: 28194443
      マウス網膜をコラーゲンゲル内でタイムラプスイメージング。
    • Interstitial fluid flow-induced growth potential and hyaluronan synthesis of fibroblasts in a fibroblast populated stretched collagen gel culture.
      Saito N, Adachi H, Tanaka H, Nakata S, Kawada N, Oofusa K, Yoshizato K.
      Biochim Biophys Acta. 2017 Jun 28;1861(9):2261-2273. PMID: 28668298
      線維芽細胞含有コラーゲンゲルを還流培養。
    • Progressive induction of hepatocyte progenitor cells in chronically injured liver.
      Tanimizu N, Ichinohe N, Yamamoto M, Akiyama H, Nishikawa Y, Mitaka T.
      Sci Rep. 2017 Jan 4;7:39990. PMID: 28051157
      Sox9陽性EpCAM陰性CD24陽性細胞や胆管細胞のコラーゲンゲル上培養。また、マトリゲルと混合してサンドイッチ培養。
    • Progranulin and granulin-like protein as novel VEGF-independent angiogenic factors derived from human mesothelioma cells.
      Eguchi R, Nakano T, Wakabayashi I.
      Oncogene. 2017 Feb 2;36(5):714-722. PMID: 27345409
      血管内皮細胞のコラーゲンゲル内三次元培養およびサンドイッチ培養。
    • Wound healing delays in α-Klotho-deficient mice that have skin appearance similar to that in aged humans - Study of delayed wound healing mechanism.
      Yamauchi M, Hirohashi Y, Torigoe T, Matsumoto Y, Yamashita K, Kayama M, Sato N, Yotsuyanagi T.
      Biochem Biophys Res Commun. 2016 May 13;473(4):845-52. PMID: 27037022
      線維芽細胞のコラーゲンゲル内三次元培養。
    • Co-expression of S100A14 and S100A16 correlates with a poor prognosis in human breast cancer and promotes cancer cell invasion.
      Tanaka M, Ichikawa-Tomikawa N, Shishito N, Nishiura K, Miura T, Hozumi A, Chiba H, Yoshida S, Ohtake T, Sugino T.
      BMC Cancer. 2015 Feb 13;15:53. PMID: 25884418
      乳がん細胞のコラーゲンゲル内三次元培養。
    • Use of biomimetic microtissue spheroids and specific growth factor supplementation to improve tenocyte differentiation and adaptation to a collagen-based scaffold in vitro.
      Theiss F, Mirsaidi A, Mhanna R, Kümmerle J, Glanz S, Bahrenberg G, Tiaden AN, Richards PJ.
      Biomaterials. 2015 Nov;69:99-109. PMID: 26283157
      腱細胞スフェロイドのコラーゲンゲル内三次元培養。
    • Recombinant human soluble thrombomodulin attenuates FK506-induced endothelial dysfunction through prevention of Akt inactivation.
      Eguchi R, Fujimori Y, Okada M, Tamaki H, Wakabayashi I, Ogawa H.
      Exp Cell Res. 2014 Apr 15;323(1):112-7. PMID: 24582967
      血管内皮細胞のコラーゲンゲル内三次元培養。
    • A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes.
      Inagawa Y, Yamada K, Yugawa T, Ohno S, Hiraoka N, Esaki M, Shibata T, Aoki K, Saya H, Kiyono T.
      Carcinogenesis. 2014 Aug;35(8):1840-6. PMID: 24858378.
      膵管細胞のコラーゲンゲル内三次元培養。
    • Differential sensitivity of epithelial cells to extracellular matrix in polarity establishment.
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      尿細管上皮細胞、乳腺上皮細胞、大腸がん細胞のコラーゲンゲル内三次元培養。
    • FK506 induces endothelial dysfunction through attenuation of Akt and ERK1/2 independently of calcineurin inhibition and the caspase pathway.
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      血管内皮細胞のコラーゲンゲル内三次元培養。
    • Dynamics of Centrosome Translocation and Microtubule Organization in Neocortical Neurons during Distinct Modes of Polarization.
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    • Nymphaeol-A Isolated from Okinawan Propolis Suppresses Angiogenesis and Induces Caspase-Dependent Apoptosis via Inactivation of Survival Signals.
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      血管内皮細胞のサンドイッチ培養。
    • Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells.
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      血管内皮細胞のサンドイッチ培養。
    • A selective inhibitor of the Rho kinase pathway, Y-27632, and its influence on wound healing in the corneal stroma.
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    • Features of wound healing shown by fibroblasts obtained from the superficial and deep dermis.
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    • Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-forming Endothelial Cells through Inactivation of Survival Signal ERK1/2.
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      血管内皮細胞のサンドイッチ培養。
    • Dermal carbonyl modification is related to the yellowish color change of photo-aged Japanese facial skin.
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      Eguchi R, Fujimori Y, Ohta T, Kunimasa K, Nakano T.
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      血管内皮細胞のコラーゲンゲル内三次元培養。
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      血管内皮細胞のサンドイッチ培養。
    • Antiangiogenic effects of indole-3-carbinol and 3,3'-diindolylmethane are associated with their differential regulation of ERK1/2 and Akt in tube-forming HUVEC.
      Kunimasa K, Kobayashi T, Kaji K, Ohta T.
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      血管内皮細胞のサンドイッチ培養。
    • Correlation between antiangiogenic activity and antioxidant activity of various components from propolis.
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      血管内皮細胞のコラーゲンゲル内三次元培養。
    • Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells.
      Eguchi R, Toné S, Suzuki A, Fujimori Y, Nakano T, Kaji K, Ohta T.
      Exp Cell Res. 2009 Jan 15;315(2):327-35. PMID: 19022247
      血管内皮細胞のサンドイッチ培養。
    • Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells.
      Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonemura S, Ohno S.
      J Cell Sci. 2009 May 15;122(Pt 10):1595-606. PMID: 19401335
      尿細管上皮細胞のコラーゲンゲル内三次元培養。
    • Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex.
      Masuda-Hirata M, Suzuki A, Amano Y, Yamashita K, Ide M, Yamanaka T, Sakai M, Imamura M, Ohno S.
      Genes Cells. 2009 Jul;14(7):835-50. PMID: 19549170
      尿細管上皮細胞のコラーゲンゲル内三次元培養。
    • Proteomic analysis of hypoxia-induced tube breakdown of an in vitro capillary model composed of HUVECs: potential role of p38-regulated reduction of HSP27.
      Eguchi R, Naitou H, Kunimasa K, Ayuzawa R, Fujimori Y, Ohashi N, Kaji K, Ohta T.
      Proteomics. 2008 Jul;8(14):2897-906. PMID: 18655027
      血管内皮細胞のサンドイッチ培養。
    • Differentiation of adipose-derived stromal vascular fraction culture cells into chondrocytes using the method of cell sorting with a mesenchymal stem cell marker.
      Ishimura D, Yamamoto N, Tajima K, Ohno A, Yamamoto Y, Washimi O, Yamada H.
      Tohoku J Exp Med. 2008 Oct;216(2):149-56.
      脂肪由来間質血管分画細胞のうち、CD105陽性細胞をコラーゲンゲル内三次元培養。
    • Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study.
      Takeuchi R, Ryo A, Komitsu N, Mikuni-Takagaki Y, Fukui A, Takagi Y, Shiraishi T, Morishita S, Yamazaki Y, Kumagai K, Aoki I, Saito T.
      Arthritis Res Ther. 2008;10(4):R77. PMID: 18616830
      軟骨細胞のコラーゲンゲル内三次元培養。
    • Suppression of tumor-induced angiogenesis by Brazilian propolis: major component artepillin C inhibits in vitro tube formation and endothelial cell proliferation.
      Ahn MR, Kunimasa K, Ohta T, Kumazawa S, Kamihira M, Kaji K, Uto Y, Hori H, Nagasawa H, Nakayama T.
      Cancer Lett. 2007 Jul 18;252(2):235-43. PMID: 17343983
      血管内皮細胞のサンドイッチ培養。
    • Hypoxia induces apoptosis of HUVECs in an in vitro capillary model by activating proapoptotic signal p38 through suppression of ERK1/2.
      Eguchi R, Suzuki A, Miyakaze S, Kaji K, Ohta T.
      Cell Signal. 2007 Jun;19(6):1121-31. PMID: 17303382
      血管内皮細胞のサンドイッチ培養。
    • Hypoxia-induced apoptosis and tube breakdown are regulated by p38 MAPK but not by caspase cascade in an in vitro capillary model composed of human endothelial cells.
      Ohta T, Eguchi R, Suzuki A, Miyakaze S, Ayuzawa R, Kaji K.
      J Cell Physiol. 2007 Jun;211(3):673-81. PMID: 17373651
      血管内皮細胞のサンドイッチ培養。
    • Atelocollagen for culture of human nucleus pulposus cells forming nucleus pulposus-like tissue in vitro: influence on the proliferation and proteoglycan production of HNPSV-1 cells.
      Sakai D, Mochida J, Iwashina T, Watanabe T, Suyama K, Ando K, Hotta T.
      Biomaterials. 2006 Jan;27(3):346-53. PMID: 16102816
      髄核細胞のコラーゲンゲル内三次元培養。
    • Effects of low-level hard laser irradiation on mineralization in three-dimensional cultured rat bone marrow cells.
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      Wenda Dai, Zhenjun Yao, Jian Dong, Naoki Kawazoe, Chi Zhang, Guoping Chen
      J Bioact Compat Poly. May 2013 vol. 28 no. 3 247-257.
      PLGAメッシュの周囲にコラーゲンスポンジを作製し、軟骨細胞の培養やマウス皮下への移植。
    • Studies on the formation mechanism and the structure of the anisotropic collagen gel prepared by dialysis-induced anisotropic gelation.
      Furusawa K, Sato S, Masumoto J, Hanazaki Y, Maki Y, Dobashi T, Yamamoto T, Fukui A, Sasaki N.
      Biomacromolecules. 2012 Jan 9;13(1):29-39. PMID: 22107030
      異方性コラーゲンゲルの作製。
    • Development of biodegradable scaffolds based on magnetically guided assembly of magnetic sugar particles.
      Hu C, Uchida T, Tercero C, Ikeda S, Ooe K, Fukuda T, Arai F, Negoro M, Kwon G.
      J Biotechnol. 2012 May 31;159(1-2):90-8. PMID:22361001
      磁性糖粒子スキャフォールドのコラーゲンコーティング。
    • Application of collagen hydrogel/sponge scaffold facilitates periodontal wound healing in class II furcation defects in beagle dogs.
      Kosen Y, Miyaji H, Kato A, Sugaya T, Kawanami M.
      J Periodontal Res. 2012 Oct;47(5):626-34. PMID:22443229
      コラーゲン溶液をコラーゲンスポンジに添加し、イヌ歯根分岐部欠損へ移植。
    • Spatial immobilization of bone morphogenetic protein-4 in a collagen-PLGA hybrid scaffold for enhanced osteoinductivity.
      Lu H, Kawazoe N, Kitajima T, Myoken Y, Tomita M, Umezawa A, Chen G, Ito Y.
      Biomaterials. 2012 Sep;33(26):6140-6. PMID:22698726
      PLGAメッシュ周囲にコラーゲンスポンジを形成。コラーゲン結合ドメイン+BMP4融合タンパク質を添加し、骨髄由来間葉系幹細胞の培養やマウスへの移植。
    • Micropatterned angiogenesis induced by poly(d,l-lactic-co-glycolic acid) mesh-structured scaffolds.
      Hwan H Oh, Hongxu Lu, Naoki Kawazoe, Guoping Chen
      J Bioact Compat Pol. March 2012 vol. 27 no. 2 97-106.
      PLGAメッシュをコラーゲンコーティングし、マウス皮下へ移植。
    • Three-dimensional culture of mouse bone marrow cells within a porous polymer scaffold: effects of oxygen concentration and stromal layer on expansion of haematopoietic progenitor cells.
      Miyoshi H, Murao M, Ohshima N, Tun T.
      J Tissue Eng Regen Med. 2011 Feb;5(2):112-8. PMID:20653040
      多孔質PVF樹脂スキャフォールドをコラーゲンコーティングし、造血前駆細胞と赤芽球を培養。
    • Ectopic Bone Induction by BMP-loaded Collagen Scaffold and Bone Marrow Stromal Cell Sheet.
      Kana INOUE, Hirofumi MIYAJI, Tsutomu SUGAYA and Masamitsu KAWANAMI
      J Oral Tissue Eng. Vol. 8 (2010) , No. 1 pp.19-29.
      コラーゲン+ゼラチンスポンジを作製。骨髄由来間葉系幹細胞シート、BMP、骨髄由来間葉系幹細胞シート+BMPをそれぞれ添加し、大腿骨と大腿二頭筋の間に移植。
    • A cell leakproof PLGA-collagen hybrid scaffold for cartilage tissue engineering.
      Kawazoe N, Inoue C, Tateishi T, Chen G.
      Biotechnol Prog. 2010 May-Jun;26(3):819-26. PMID: 20039440
      PLGAメッシュ周囲にコラーゲンスポンジを作製し、間葉系幹細胞を培養。
    • Efficacy of Tooth Extraction Wound Protection Made of Atelocollagen Sponge (TRE-641): A Pilot Study in Dogs
      Makoto Hirota), Nobuyuki Mizuki), Shinjiro Aoki), Susumu Omura), Kei Watanuki), Tomomichi Ozawa), Toshinori Iwai), Yoshiro Matsui) and Iwai Tohnai)
      Journal of Hard Tissue Biology Vol. 18 (2009) , No. 2 pp.89-94.
      コラーゲン+セラチンスポンジを作製し、イヌ抜歯部へ移植。
    • Three-dimensional Cultures of Rat Pancreatic RIN-5F Cells in Porous PLGA-collagen Hybrid Scaffolds.
      Naoki Kawazoe, Xiaoting Lin, Tetsuya Tateishi, and Guoping Chen.
      J Bioact Compat Pol. January 2009 24: 25-42.
      PLGAメッシュ周囲にコラーゲンスポンジを形成し、膵臓β細胞を培養。
    • Stimulating effects of fibroblast growth factors on hepatic function of fetal liver cells synergistically with oncostatin M in three-dimensional culture.
      Minagawa K, Koyama T, Miyoshi H.
      J Biosci Bioeng. 2009 Mar;107(3):307-11. PMID: 19269598
      多孔質PVF樹脂スキャフォールドをコラーゲンコーティングし、肝細胞を培養。
    • Recombinant human bone morphogenetic protein-2/atelocollagen composite as a new material for ossicular reconstruction.
      Takeuchi A, Tsujigiwa H, Murakami J, Kawasaki A, Takeda Y, Fukushima K, Rodriguez AP, Nagatsuka H, Yamada M, Nishizaki K.
      J Biomed Mater Res A. 2009 Apr;89(1):36-45. PMID: 18404714
      コラーゲン+BMP-2スポンジを作製し、ラット耳小骨欠損部へ移植。
    • Multiwalled carbon nanotube coating on titanium.
      Terada M, Abe S, Akasaka T, Uo M, Kitagawa Y, Watari F.
      Biomed Mater Eng. 2009;19(1):45-52. PMID: 19458445
      チタンプレートをコラーゲンコーティングし、骨芽細胞様細胞を培養。
    • Development of biodegradable scaffolds based on patient-specific arterial configuration.
      Uchida T, Ikeda S, Oura H, Tada M, Nakano T, Fukuda T, Matsuda T, Negoro M, Arai F.
      J Biotechnol. 2008 Jan 20;133(2):213-8. PMID: 17868940
      コラーゲン+PLACLスキャフォールドを作製し、血管内皮細胞を培養。
    • Evaluation of diaphragmatic hernia repair using PLGA mesh-collagen sponge hybrid scaffold: an experimental study in a rat model.
      Urita Y, Komuro H, Chen G, Shinya M, Saihara R, Kaneko M.
      Pediatr Surg Int. 2008 Sep;24(9):1041-5. PMID: 18668247
      PLGAメッシュ周囲にコラーゲンスポンジを作製。間葉系幹細胞を播種し、マウス横隔膜欠損部へ移植。
    • Establishment of Nanofiber Preparation Technique by Electrospinning.
      Y Yamashita, F Ko, H Miyake, A Higashiyama
      Sen'I Gakkaishi. 2008 Vol. 64 No. 1 P 24-28.
      コラーゲン溶液を凍結乾燥後、再溶解してエレクトロスピニングでファイバーを作製。
    • In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding.
      Yokota T, Ichikawa H, Matsumiya G, Kuratani T, Sakaguchi T, Iwai S, Shirakawa Y, Torikai K, Saito A, Uchimura E, Kawaguchi N, Matsuura N, Sawa Y.
      J Thorac Cardiovasc Surg. 2008 Oct;136(4):900-7. PMID: 18954628
      PGA/PLLA血管グラフト周囲にコラーゲンスポンジを作製し、イヌ頸動脈へ移植。
    • Effects of oncostatin M on secretion of vascular endothelial growth factor and reconstruction of liver-like structure by fetal liver cells in monolayer and three-dimensional cultures.
      Ehashi T, Koyama T, Ookawa K, Ohshima N, Miyoshi H.
      Biomed Mater Res A. 2007 Jul;82(1):73-9. PMID: 17269136
      多孔質PVF樹脂スキャフォールドをコラーゲンコーティングし、肝細胞を培養。
    • Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold biomaterials.
      Hamada Y, Egusa H, Kaneda Y, Hirata I, Kawaguchi N, Hirao T, Matsumoto T, Yao M, Daito K, Suzuki M, Yatani H, Daito M, Okazaki M, Matsuura N.
      Dent Mater J. 2007 Jul;26(4):487-92. PMID: 17886451
      コラーゲン+CO3Apスポンジを作製し、骨髄由来間質細胞を培養。
    • Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds.
      Hirata I, Nomura Y, Ito M, Shimazu A, Okazaki M.
      J Artif Organs. 2007;10(4):212-7. PMID: 18071850
      HApフレームで囲ったコラーゲン+CO3Apスポンジを作製し、骨芽細胞を培養。
    • Ultrastructural study on the specific binding of genetically engineered epidermal growth factor to type I collagen fibrils.
      Kato K, Sato H, Iwata H.
      Bioconjug Chem. 2007 Nov-Dec;18(6):2137-43. PMID: 17894450
      セファロースビーズをコラーゲンコーティングし、コラーゲン結合ドメイン+EGF融合タンパク質を結合させて電顕観察。
    • Osteochondral tissue engineering using a PLGA–collagen hybrid mesh.
      Guoping Chen, Junzo Tanaka, Tetsuya Tateishi.
      Materials Science and Engineering: C, Volume 26, Issue 1, January 2006, Pages 124-129.
      PLGAメッシュ周囲にコラーゲンスポンジを作製し、骨髄由来間質細胞を培養。
    • Polymer Scaffolds for Tissue Engineering.
      G. P. Chen, M. Tanaka, T. Tateishi.
      Advances in Science and Technology, Vol. 49, pp. 136-141, Oct. 2006.
      コラーゲン+AHAsスポンジあるいはAHAsメッシュの周囲にコラーゲンスポンジを作製。線維芽細胞、腱細胞、軟骨細胞、間葉系幹細胞を培養。
    • Preparation of a biphasic scaffold for osteochondral tissue engineering.
      Guoping Chen, Takashi Sato, Junzo Tanaka, Tetsuya Tateishi.
      Materials Science and Engineering: C, Volume 26, Issue 1, January 2006, Pages 118-123.
      コラーゲンスポンジとコラーゲン+PLGAスポンジの二層スキャフォールドを作製し、イヌ骨軟骨欠損部へ移植。
    • Three-dimensional culture of porcine fetal liver cells for a bioartificial liver.
      Ehashi T, Ohshima N, Miyoshi H.
      J Biomed Mater Res A. 2006 Apr;77(1):90-6. PMID: 16355413
      多孔質PVF樹脂スキャフォールドをコラーゲンコーティングし、肝細胞を培養。
    • Osteoblast Behavior at the Surface of CO3Ap-Collagen Sponges.
      M. Okazaki, Y. Tieliewuhan, I. Hirata.
      Key Engineering Materials, Vols. 309-311, pp. 989-992, May. 2006.
      コラーゲン+CO3Apスポンジを作製し、骨芽細胞様細胞を培養。
    • Albumin and urea production by hepatocytes cultured on polyurethane foaming membranes coated with extracellular matrix.
      Akon Higuchi, Masuhiro Satoh, Kenichi Kobayashi, Chong Su Cho, Toshihiro Akaike, Tae Moon Tak, Satsuki Egashira, Yuki Matsuoka, Shizue Hayashi Natori.
      Journal of Membrane Science, Volume 280, Issues 1–2, 1 September 2006, Pages 983-989.
      ポリウレタン発泡体膜をコラーゲンコーティングし、肝細胞を培養。
    • Detailed consideration of physicochemical properties of CO3apatites as biomaterials in relation to carbonate content using ICP, X-ray diffraction, FT-IR, SEM, and HR-TEM.
      Yokota R, Hayashi H, Hirata I, Miake Y, Yanagisawa T, Okazaki M.
      Dent Mater J. 2006 Sep;25(3):597-603. PMID: 17076333
      コラーゲン+CO3Apスポンジを作製し、骨芽細胞様細胞を培養。
    • Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh.
      Chen G, Sato T, Ohgushi H, Ushida T, Tateishi T, Tanaka J.
      Biomaterials. 2005 May;26(15):2559-66. PMID: 15585258
      PLGAメッシュ周囲にコラーゲンスポンジを作製し、線維芽細胞を培養。
    • Albumin and urea production by hepatocytes cultured on extracellular matrix proteins-conjugated poly(vinyl alcohol) membranes.
      Higuchi A, Kurihara M, Kobayashi K, Cho CS, Akaike T, Hara M.
      J Biomater Sci Polym Ed. 2005;16(7):847-60. PMID: 16128292
      PVA-EA膜をコラーゲンコーティングし、肝細胞を培養。
    • Temperature-dependent cell detachment on Pluronic gels.
      Higuchi A, Yamamoto T, Sugiyama K, Hayashi S, Tak TM, Nakagawa T.
      Biomacromolecules. 2005 Mar-Apr;6(2):691-6. PMID: 15762631 コラーゲン+Pluronic F127あるいはコラーゲン+フィブロネクチン+Pluronic F127ゲルを作製し、線維芽細胞や血管内皮細胞を培養。
    • SEM observation of collagen fibrils secreted from the body surface of osteoblasts on a CO3apatite-collagen sponge.
      Hirata I, Nomura Y, Tabata H, Miake Y, Yanagisawa T, Okazaki M.
      Dent Mater J. 2005 Sep;24(3):460-4. PMID: 16279740
      コラーゲン+CO3Apスポンジを作製し、骨芽細胞様細胞を培養。
    • Effects of biomaterials on cell adhesion of human periodontal ligament fibroblasts and fibronectin adsorption.
      Y. Iwamatsu-Kobayashi, D. Nishihara, M. Hirata, K. Kindaichi, M. Komatsu.
      International Congress Series, Volume 1284, September 2005, Pages 334-335.
      チタンディスクやポリスチレンディスクをコラーゲンコーティングし、線維芽細胞を培養。
    • Cell adhesion of bone marrow cells, chondrocytes, ligament cells and synovial cells on a PLGA–collagen hybrid mesh.
      Guoping Chen, Dechang Liu, Norio Maruyama, Hajime Ohgushi, Junzo Tanaka, Tetsuya Tateishi.
      Materials Science and Engineering: C. Volume 24, Issues 6–8, 1 December 2004, Pages 867–873.
      PLGAメッシュ周囲にコラーゲンスポンジを作製し、骨髄由来細胞、軟骨細胞、靭帯由来細胞、滑膜由来細胞を培養。
    • Application of PLGA-collagen hybrid mesh for three-dimensional culture of canine anterior cruciate ligament cells.
      Guoping Chen, Takashi Sato, Masataka Sakane, Hajime Ohgushi, Takashi Ushida, Junzo Tanaka, Tetsuya Tateishi.
      Materials Science and Engineering: C. Volume 24, Issues 6–8, 1 December 2004, Pages 861–866.
      PLGAメッシュ周囲にコラーゲンスポンジを作製し、前十字靭帯由来細胞を培養。
    • Regeneration of cartilage tissue by combination of canine chondrocyte and a hybrid mesh scaffold.
      Guoping Chen, Takashi Sato, Takashi Ushida, Rei Hirochika, Naoyuki Ochiai, Tetsuya Tateishi.
      Materials Science and Engineering: C. Volume 24, Issue 3, 1 April 2004, Pages 373–378.
      PLGAメッシュ周囲にコラーゲンスポンジを形成。軟骨細胞を培養し、マウス皮下へ移植。
    • Characterization of CO3Ap-collagen sponges using X-ray high-resolution microtomography.
      Itoh M, Shimazu A, Hirata I, Yoshida Y, Shintani H, Okazaki M.
      Biomaterials. 2004 Jun;25(13):2577-83. PMID: 14751743
      コラーゲン+CO3Apスポンジを作製し、骨芽細胞様細胞を培養。
    • Osteoblast proliferation behavior and bone formation on and in CO3apatite-collagen sponges with a porous hydroxyapatite frame.
      Tieliewuhan Y, Hirata I, Sasaki A, Minagi H, Okazaki M.
      Dent Mater J. 2004 Sep;23(3):258-64. PMID: 15510851
      HApフレームで囲ったコラーゲン+CO3Apスポンジを作製し、骨芽細胞様細胞を培養。
    • Custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet.
      Tsuchiya K, Mori T, Chen G, Ushida T, Tateishi T, Matsuno T, Sakamoto M, Umezawa A.
      Cell Tissue Res. 2004 May;316(2):141-53. PMID: 14999559
      PLGAメッシュ周囲にコラーゲンスポンジを作製。骨芽細胞を播種し、マウス頭蓋骨欠損部へ移植。
    • Bone augmentation using rhGDF-5-collagen composite.
      Kuniyasu H, Hirose Y, Ochi M, Yajima A, Sakaguchi K, Murata M, Pohl J.
      Clin Oral Implants Res. 2003 Aug;14(4):490-9. PMID: 12869012
      コラーゲン+GDF-5スポンジを作製し、ラット頭蓋骨骨膜下へ移植。
    • The Toughness-Enhanced Atelocollagen Double-Network Gel for Biomaterials.
      Tsuyukubo A, Kubota R, Sato Y, Fujimoto I.
      Polymers (Basel). 2024 Jan 19;16(2):283. PMID: 38276691.
      コラーゲンとPDMAAmのダブルネットワークゲルを作製し、線維芽細胞をゲル上培養。
    • Perturbed collagen metabolism underlies lymphatic recanalization failure in Gata2 heterozygous deficient mice.
      Watanabe-Asaka T, Hayashi M, Harada T, Uemura S, Takai J, Nakamura Y, Moriguchi T, Kawai Y.
      J Biochem. 2024 Jan 2:mvad122. doi: 10.1093/jb/mvad122. Epub ahead of print. PMID: 38168819.
      マウス胎児線維芽細胞の培養中にコラーゲン溶液を添加。また、マウス膝窩リンパ節切断部へコラーゲン溶液を投与し、リンパ菅の再生を評価。
    • The EGF Motif With CXDXXXXYXCXC Sequence Suppresses Fibrosis in a Mouse Skin Wound Model.
      Kitano H, Ishikawa T, Masaoka Y, Komiyama K, Takahashi M, Hidai C.
      In Vivo. 2023 Jul-Aug;37(4):1486-1497. PMID: 37369508.
      線維芽細胞のコラーゲンゲル上培養。
    • Efficient Liposome Loading onto Surface of Mesenchymal Stem Cells via Electrostatic Interactions for Tumor-Targeted Drug Delivery.
      Kono Y, Kamino R, Hirabayashi S, Kishimoto T, Kanbara H, Danjo S, Hosokawa M, Ogawara KI.
      Biomedicines. 2023 Feb 14;11(2):558. PMID: 36831094.
      磁性リポソームとコラーゲンの複合体を作製し、間葉系幹細胞表面を修飾して尾静脈投与。
    • Suppression of Phagocytic Activity Leads to the Efficient Surface Modification of Macrophages with Liposomes for Developing a Biomimetic Drug Delivery System.
      Kono Y, Uesugi N, Saegusa M, Onishi K, Hosokawa M, Ogawara KI.
      Biol Pharm Bull. 2023;46(9):1347-1351. PMID: 37661414.
      磁性リポソームとコラーゲンの複合体を作製し、マクロファージへ添加。
    • Stiffness-Modulation of Collagen Gels by Genipin-Crosslinking for Cell Culture.
      Ishihara S, Kurosawa H, Haga H.
      Gels. 2023 Feb 10;9(2):148. PMID: 36826318.
      コラーゲンゲルをゲニピンで架橋し、ゲル上で肺がん細胞や間葉系幹細胞を培養。
    • Voltammetric Sensing of Soybean Agglutinin Using an Electrode Modified with Electron-transfer, Carbohydrate-mimetic/Cross-linker-peptide-collagen Film
      S. Ishizaki, H. Kuramitz, K. Sugawara.
      Electroanalysis 2022, 34, 464.
      電極のコラーゲンコーティング。
    • Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process
      Takayuki Narita, Misaki Kondo, and Yushi Oishi
      ACS Omega 2022 7 (1), 1014-1020
      バンドパターンのあるコラーゲンゲルを作製。
    • Mechanical performance of collagen gels is dependent on purity, α1/α2 ratio, and telopeptides.
      Slyker L, Diamantides N, Kim J, Bonassar LJ.
      J Biomed Mater Res A. 2022 Jan;110(1):11-20. PMID: 34236763.
      ウシ皮、ウシ腱、ラット尾のネイティブコラーゲンやアテロコラーゲンの物性比較。
    • Magnetization of mesenchymal stem cells using magnetic liposomes enhances their retention and immunomodulatory efficacy in mouse inflamed skeletal muscle.
      Kono Y, Takegaki J, Ohba T, Matsuda K, Negoro R, Fujita S, Fujita T.
      Int J Pharm. 2021 Jan 30;596:120298. PMID: 33529784.
    • Effects of the prevention of medication-related osteonecrosis of the jaw by local administration of a dental pulp stem cell-conditioned medium to the rat tooth extraction socket.
      Kushiro H, Takahashi H, Tanaka A.
      Odontology. 2021 Oct;109(4):836-844. PMID: 33907964.
      コラーゲン溶液と歯髄幹細胞培養上清を混合してビーズを作製し、ラット抜歯窩へ移植。
    • Effect of Amelotin on Bone Growth in the Murine Calvarial Defect Model.
      Tanaka D, Ikeda Y, Ikeda E, Yokose M, Ganss B, Iwata T.
      Ann Biomed Eng. 2021 Dec;49(12):3676-3684. PMID: 34608582.
      コラーゲンメンブレンの上にAmelotin含有コラーゲンゲルを作製し、マウス頭蓋骨欠損部へ移植。
    • TGF-β promotes fetal gene expression and cell migration velocity in a wound repair model of untransformed intestinal epithelial cells.
      Anzai S, Kawamoto A, Nagata S, Takahashi J, Kawai M, Kuno R, Kobayashi S, Watanabe S, Suzuki K, Shimizu H, Hiraguri Y, Takeoka S, Sugihara HY, Yui S, Oshima S, Watanabe M, Okamoto R.
      Biochem Biophys Res Commun. 2020 Apr 9;524(3):533-541. PMID: 32014254
      コラーゲンゲル上で小腸オルガノイド由来腸管上皮細胞を培養。
    • Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. Hayashi S, Nakamura T, Motooka Y, Ito F, Jiang L, Akatsuka S, Iwase A, Kajiyama H, Kikkawa F, Toyokuni S.
      Redox Biol. 2020 Sep 15;37:101726. PMID: 32961443
      子宮組織を含むコラーゲン溶液を卵嚢内に移植。
    • GPR91 antagonist and TGF-β inhibitor suppressed collagen production of high glucose and succinate induced HSC activation.
      Sakai M, Sumiyoshi T, Aoyama T, Urayama K, Yoshimura R.
      Biochem Biophys Res Commun. 2020 Sep 17;530(2):362-366. PMID: 32798017.
      肝星細胞のコラーゲンゲル上培養。
    • Fabrication of a cell-recognition/electron-transfer/cross-linker, peptide-immobilized electrode for the sensing of K562 cells.
      Sugawara K, Ishizaki S, Kodaira K, Kuramitz H, Kadoya T.
      Anal Chim Acta. 2020;1116:53-61. PMID: 32389189
      コラーゲンや細胞センシングペプチドを付加した電極を作成。
    • In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair.
      Khalilifar MA, Baghaban Eslaminejad MR, Ghasemzadeh M, Hosseini S, Baharvand H.
      Cell J. 2019 Jul;21(2):150-160. PMID: 30825288.
      骨髄由来間葉系幹細胞あるいは耳由来間葉系幹細胞をウサギ軟骨欠損モデルへ移植。
    • Temperature dependence of synchronized beating of cultured neonatal rat heart-cell networks with increasing age measured by multi-electrode arrays.
      Uchida T, Kitora R, Gohara K (2018)
      Trends Med 18, Volume 18(4): 1-10.
      微小電極アレイ用プレートをコラーゲンコーティングし、心臓由来細胞を培養。
    • Spontaneous acquisition of infinite proliferative capacity by a rabbit corneal endothelial cell line with maintenance of phenotypic and physiological characteristics.
      Kageyama T, Hayashi R, Hara S, Yoshikawa K, Ishikawa Y, Yamato M, Nishida K.
      J Tissue Eng Regen Med. 2017 Apr;11(4):1057-1064. PMID: 25758102
      角膜内皮細胞のコラーゲンゲル上培養。
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    • Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels.
      Nagasaka A, Shinoda T, Kawaue T, Suzuki M, Nagayama K, Matsumoto T, Ueno N, Kawaguchi A and Miyata T.
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      フェレットやマウスの大脳半球をコラーゲンゲルに包埋し、AFMや共焦点顕微鏡で観察。
    • Interstitial fluid pressure regulates collective invasion in engineered human breast tumors via Snail, vimentin, and E-cadherin.
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      Integr Biol (Camb). 2016 Mar 14;8(3):319-31. PMID: 26853861
      針の周囲にコラーゲンゲルを形成させ、針を除去した空洞に乳がん細胞を播種してゲル内三次元培養。
    • Mammary Branching Morphogenesis Requires Reciprocal Signaling by Heparanase and MMP-14.
      Gomes AM, Bhat R, Correia AL, Mott JD, Ilan N, Vlodavsky I, Pavão MS, Bissell M.
      J Cell Biochem. 2015 Aug;116(8):1668-79. PMID: 25735873
      乳腺上皮細胞のコラーゲンゲル内三次元培養。
    • Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices.
      Vicens-Zygmunt V, Estany S, Colom A, Montes-Worboys A, Machahua C, Sanabria AJ, Llatjos R, Escobar I, Manresa F, Dorca J, Navajas D, Alcaraz J, Molina-Molina M.
      Respir Res. 2015 Jul 1;16:82. PMID: 26126411
      線維芽細胞含有あるいは非含有コラーゲンゲルを用いた糖化評価。
    • Three-dimensional traction force microscopy of engineered epithelial tissues.
      Piotrowski AS, Varner VD, Gjorevski N, Nelson CM.
      Methods Mol Biol. 2015;1189:191-206. PMID: 25245695
      蛍光ビーズ含有コラーゲン溶液にPDMSスタンプを配置し、ゲル化。PDMSスタンプを除去した空洞に上皮細胞を播種し、蛍光ビーズ含有コラーゲンゲルで覆って培養。
    • Response of Human Skin Fibroblasts to Mechanical Stretch in Wound Healing Process Analyzed Using a Three-Dimensional Culture Model
      Kaori Shikano, Keisuke Chiba, Shogo Miyata.
      Advanced Biomedical Engineering. Vol. 4 (2015) p. 170-178.
      細胞不含コラーゲンゲルの周囲を線維芽細胞含有コラーゲンゲルで囲い、伸展培養。
    • Oxygen diffusion and consumption in extracellular matrix gels: Implications for designing three-dimensional cultures.
      Colom A, Galgoczy R, Almendros I, Xaubet A, Farré R, Alcaraz J.
      J Biomed Mater Res A. 2014 Aug;102(8):2776-84. PMID: 24027235
      肺胞基底上皮腺がん細胞をコラーゲンゲル内三次元培養し、酸素拡散を評価。
    • A spectrophotometer-based diffusivity assay reveals that diffusion hindrance of small molecules in extracellular matrix gels used in 3D cultures is dominated by viscous effects.
      Galgoczy R, Pastor I, Colom A, Giménez A, Mas F, Alcaraz J.
      Colloids Surf B Biointerfaces. 2014 Aug 1;120:200-7. PMID: 24916283
      細胞含有ゲルの物質拡散を評価。
    • A novel three-dimensional cell culture method to analyze epidermal cell differentiation in vitro.
      Okugawa Y, Hirai Y.
      Methods Mol Biol. 2014;1195:183-90. PMID: 24281867
      角化細胞をスフェロイド培養して得られた細胞凝集塊のコラーゲンゲル内培養。
    • Tissue-culture light sheet fluorescence microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions.
      Pampaloni F, Berge U, Marmaras A, Horvath P, Kroschewski R, Stelzer EH
      Integr Biol (Camb). 2014 Oct;6(10):988-98. PMID: 25183478
      尿細管上皮細胞をコラーゲンゲル内三次元培養し、タイムラプスイメージング。
    • Age-related disruption of autophagy in dermal fibroblasts modulates extracellular matrix components.
      Tashiro K, Shishido M, Fujimoto K, Hirota Y, Yo K, Gomi T, Tanaka Y.
      Biochem Biophys Res Commun. 2014 Jan 3;443(1):167-72. PMID: 24287182
      線維芽細胞のコラーゲンゲル内三次元培養。
    • The hemopexin domain of MMP3 is responsible for mammary epithelial invasion and morphogenesis through extracellular interaction with HSP90β.
      Correia AL, Mori H, Chen EI, Schmitt FC, Bissell MJ.
      Genes Dev. 2013 Apr 1;27(7):805-17. PMID: 23592797
      乳腺上皮のコラーゲンゲル内器官培養。
    • Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK activation and mammary branching morphogenesis via binding to integrin β1.
      Mori H, Lo AT, Inman JL, Alcaraz J, Ghajar CM, Mott JD, Nelson CM, Chen CS, Zhang H, Bascom JL, Seiki M, Bissell MJ.
      Development. 2013 Jan;140(2):343-52. PMID: 23250208
      乳腺上皮細胞のコラーゲンゲル内三次元培養。
    • Extracellular epimorphin modulates epidermal differentiation signals mediated by epidermal growth factor receptor.
      Okugawa Y, Hirai Y.
      J Dermatol Sci. 2013 Mar;69(3):236-42. PMID: 23219092
      角化細胞をスフェロイド培養して得られた細胞凝集塊のコラーゲンゲル内三次元培養。
    • PI3K regulates branch initiation and extension of cultured mammary epithelia via Akt and Rac1 respectively.
      Zhu W, Nelson CM.
      Dev Biol. 2013 Jul 15;379(2):235-45. PMID: 23665174
      乳腺上皮細胞のコラーゲンゲル内三次元培養。
    • Knockdown of electron transfer flavoprotein β subunit reduced TGF-β-induced α-SMA mRNA expression but not COL1A1 in fibroblast-populated three-dimensional collagen gel cultures.
      Hirokawa S, Shimanuki T, Kitajima H, Nishimori Y, Shimosaka M.
      J Dermatol Sci. 2012 Dec;68(3):179-86. PMID: 23068445
      線維芽細胞のコラーゲンゲル内三次元培養。
    • Modulation of invasive phenotype by interstitial pressure-driven convection in aggregates of human breast cancer cells.
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      針の周囲にコラーゲンゲルを形成させ、針を除去した空洞に乳がん細胞を播種してゲル内三次元培養。
    • Collective epithelial cell invasion overcomes mechanical barriers of collagenous extracellular matrix by a narrow tube-like geometry and MMP14-dependent local softening.
      Alcaraz J, Mori H, Ghajar CM, Brownfield D, Galgoczy R, Bissell MJ.
      Integr Biol (Camb). 2011 Dec;3(12):1153-66. PMID:21993836
      乳腺上皮細胞のコラーゲンゲル内三次元培養。
    • The metastasis-promoting protein S100A4 regulates mammary branching morphogenesis.
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      Dev Biol. 2011 Apr 15;352(2):181-90. PMID: 21195708
      細胞不含コラーゲンゲルを作製し、乳腺上皮細胞あるいは乳腺組織含有コラーゲンゲルを重層して三次元培養。
    • Role of tumour necrosis factor-α (TNFα) in the functional properties of hyalocytes.
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      Br J Ophthalmol. 2011 Feb;95(2):261-5. PMID: 21030411 .
      硝子体細胞のコラーゲンゲル内三次元培養。
    • Identification of ETFB as a candidate protein that participates in the mechanoregulation of fibroblast cell number in collagen gel culture.
      Hirokawa S, Shimanuki T, Kitajima H, Nishimori Y, Shimosaka M.
      J Dermatol Sci. 2011 Nov;64(2):119-26. PMID: 21903359
      線維芽細胞のコラーゲンゲル内三次元培養。
    • Novel migrating mouse neural crest cell assay system utilizing P0-Cre/EGFP fluorescent time-lapse imaging.
      Kawakami M, Umeda M, Nakagata N, Takeo T, Yamamura K.
      BMC Dev Biol. 2011 Nov 9;11:68. PMID: 22070366
      マウス胚をサンドイッチ培養し、タイムラプスイメージング。
    • Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis.
      Lee K, Gjorevski N, Boghaert E, Radisky DC, Nelson CM.
      EMBO J. 2011 May 24;30(13):2662-74. PMID: 21610693
      乳腺上皮細胞をコラーゲンゲル内三次元培養し、タイムラプスイメージング。
    • Mammary branch initiation and extension are inhibited by separate pathways downstream of TGFβ in culture.
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      Exp Cell Res. 2011 Aug 1;317(13):1872-84. PMID: 21459084
      乳腺上皮細胞のコラーゲンゲル内三次元培養。
    • Endogenous patterns of mechanical stress are required for branching morphogenesis.
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      Integr Biol (Camb). 2010 Sep;2(9):424-34. PMID: 20717570
      コラーゲン溶液にPDMSスタンプを配置し、ゲル化。PDMSスタンプを除去した空洞に乳腺上皮細胞を播種し、コラーゲンゲルで覆って培養。
    • Epimorphin-derived peptide antagonists remedy epidermal parakeratosis triggered by unsaturated fatty acid.
      Okugawa Y, Bascom JJ, Hirai Y.
      J Dermatol Sci. 2010 Sep;59(3):176-83. PMID: 20688483
      角化細胞のスフェロイドをコラーゲンゲル内三次元培養。
    • Madin-Darby canine kidney cells are increased in aerobic glycolysis when cultured on flat and stiff collagen-coated surfaces rather than in physiological 3-D cultures.
      Pampaloni F, Stelzer EH, Leicht S, Marcello M.
      Proteomics. 2010 Oct;10(19):3394-413. PMID: 20718008
      尿細管上皮細胞のコラーゲンゲル内三次元培養。ディッシュのコーティングも。
    • Adipose stroma induces branching morphogenesis of engineered epithelial tubules.
      Pavlovich AL, Manivannan S, Nelson CM.
      Tissue Eng Part A. 2010 Dec;16(12):3719-26. PMID: 20649458
      線維芽細胞のコラーゲンゲル内三次元培養。
    • Neurotrophic effect of gonadotropin-releasing hormone on neurite extension and neuronal migration of embryonic gonadotropin-releasing hormone neurons in chick olfactory nerve bundle culture.
      Kanaho Y, Enomoto M, Endo D, Maehiro S, Park MK, Murakami S.
      J Neurosci Res. 2009 Aug 1;87(10):2237-44. PMID: 19301422
      コラーゲン+マトリゲルからなるゲル内で、嗅覚神経束を培養。
    • Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins.
      Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T.
      Diabetes. 2008 Oct;57(10):2784-93. PMID: 18599521
      Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17504-9. PMID: 18952846
      硝子体細胞をコラーゲンゲル内三次元培養。
    • Hic-5, an adaptor protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo.
      Kim-Kaneyama JR, Wachi N, Sata M, Enomoto S, Fukabori K, Koh K, Shibanuma M, Nose K.
      Biochem Biophys Res Commun. 2008 Nov 28;376(4):682-7. PMID: 18812162
      血管平滑筋細胞のコラーゲンゲル内三次元培養。
    • Role of TGF-beta in proliferative vitreoretinal diseases and ROCK as a therapeutic target.
      Kita T, Hata Y, Arita R, Kawahara S, Miura M, Nakao S, Mochizuki Y, Enaida H, Goto Y, Shimokawa H, Hafezi-Moghadam A, Ishibashi T.
      Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17504-9. PMID: 18952846
      硝子体細胞をコラーゲンゲル内三次元培養。
    • Overexpression of extracellular epimorphin leads to impaired epidermal differentiation in HaCaT keratinocytes.
      Okugawa Y, Hirai Y.
      J Invest Dermatol. 2008 Aug;128(8):1884-93. PMID: 18273050
      角化細胞の細胞塊をコラーゲンゲル内三次元培養。
    • Non-classical export of epimorphin and its adhesion to alphav-integrin in regulation of epithelial morphogenesis.
      Hirai Y, Nelson CM, Yamazaki K, Takebe K, Przybylo J, Madden B, Radisky DC.
      J Cell Sci. 2007 Jun 15;120(Pt 12):2032-43. PMID: 17535848
      乳腺上皮細胞のコラーゲンゲル内三次元培養。
    • Functional characteristics of connective tissue growth factor on vitreoretinal cells.
      Kita T, Hata Y, Miura M, Kawahara S, Nakao S, Ishibashi T.
      Diabetes. 2007 May;56(5):1421-8. PMID: 17303801
      硝子体細胞や網膜色素上皮細胞のコラーゲンゲル内三次元培養。
    • Critical role of the Rho-kinase pathway in TGF-beta2-dependent collagen gel contraction by retinal pigment epithelial cells.
      Miura M, Hata Y, Hirayama K, Kita T, Noda Y, Fujisawa K, Shimokawa H, Ishibashi T.
      Exp Eye Res. 2006 May;82(5):849-59. PMID: 16310190
      網膜色素上皮細胞のコラーゲンゲル内三次元培養。
    • Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity.
      Yamanaka T, Horikoshi Y, Izumi N, Suzuki A, Mizuno K, Ohno S.
      J Cell Sci. 2006 May 15;119(Pt 10):2107-18. PMID: 16638806
      尿細管上皮細胞のコラーゲンゲル内三次元培養。
    • The effect of hyaluronic acid with different molecular weights on collagen crosslink synthesis in cultured chondrocytes embedded in collagen gels.
      Abe M, Takahashi M, Nagano A.
      J Biomed Mater Res A. 2005 Nov 1;75(2):494-9. PMID: 16092114
      軟骨細胞のコラーゲンゲル内三次元培養。
    • Repulsive guidance of axons of spinal sensory neurons in Xenopus laevis embryos: roles of Contactin and notochord-derived chondroitin sulfate proteoglycans.
      Fujita N, Nagata S.
      Dev Growth Differ. 2005 Sep;47(7):445-56. PMID: 16179071
      脊髄や脊索のコラーゲンゲル内三次元培養。
    • Leukemia inhibitory factor induces multi-lineage differentiation of adult stem-like cells in kidney via kidney-specific cadherin 16.
      Hishikawa K, Marumo T, Miura S, Nakanishi A, Matsuzaki Y, Shibata K, Kohike H, Komori T, Hayashi M, Nakaki T, Nakauchi H, Okano H, Fujita T.
      Biochem Biophys Res Commun. 2005 Mar 4;328(1):288-91. PMID: 15670782
      腎臓サイドポピュレーション細胞のコラーゲンゲル内三次元培養。
    • Extraocular dorsal signal affects the developmental fate of the optic vesicle and patterns the optic neuroepithelium.
      Kagiyama Y, Gotouda N, Sakagami K, Yasuda K, Mochii M, Araki M.
      Dev Growth Differ. 2005 Oct;47(8):523-36. PMID: 16287484
      ニワトリ胚由来眼胞含有コラーゲンゲルをセルカルチャーインサート上に添加し、ゲル内培養。
    • Uni-axial stretching regulates intracellular localization of Hic-5 expressed in smooth-muscle cells in vivo.
      Kim-Kaneyama JR, Suzuki W, Ichikawa K, Ohki T, Kohno Y, Sata M, Nose K, Shibanuma M.
      J Cell Sci. 2005 Mar 1;118(Pt 5):937-49. PMID: 15713747
      線維芽細胞のコラーゲンゲル内三次元培養。
    • Subculture of chondrocytes on a collagen type I-coated substrate with suppressed cellular dedifferentiation.
      Kino-Oka M, Yashiki S, Ota Y, Mushiaki Y, Sugawara K, Yamamoto T, Takezawa T, Taya M.
      Tissue Eng. 2005 Mar-Apr;11(3-4):597-608. PMID: 15869436
      軟骨細胞のコラーゲンゲル内三次元培養。
    • Repair of articular cartilage defect by autologous transplantation of basic fibroblast growth factor gene-transduced chondrocytes with adeno-associated virus vector.
      Yokoo N, Saito T, Uesugi M, Kobayashi N, Xin KQ, Okuda K, Mizukami H, Ozawa K, Koshino T.
      Arthritis Rheum. 2005 Jan;52(1):164-70. PMID: 15641065
      軟骨細胞をコラーゲンゲル内三次元培養し、ウサギ軟骨欠損部へ移植。
    • Activin A augments vascular endothelial growth factor activity in promoting branching tubulogenesis in hepatic sinusoidal endothelial cells.
      Endo D, Kogure K, Hasegawa Y, Maku-uchi M, Kojima I.
      J Hepatol. 2004 Mar;40(3):399-404. PMID: 15123352
      類洞内皮細胞のコラーゲンゲル内三次元培養。
    • The involvement of the rho-kinase pathway and its regulation in cytokine-induced collagen gel contraction by hyalocytes.
      Hirayama K, Hata Y, Noda Y, Miura M, Yamanaka I, Shimokawa H, Ishibashi T.
      Invest Ophthalmol Vis Sci. 2004 Nov;45(11):3896-903. PMID: 15505034
      硝子体細胞のコラーゲンゲル内三次元培養。
    • P311 accelerates nerve regeneration of the axotomized facial nerve.
      Fujitani M, Yamagishi S, Che YH, Hata K, Kubo T, Ino H, Tohyama M, Yamashita T.
      J Neurochem. 2004 Nov;91(3):737-44. PMID: 15485502
      後根神経節(DRG)のコラーゲンゲル内培養。
    • Vascular endothelial growth factor- and thrombin-induced termination factor, Down syndrome critical region-1, attenuates endothelial cell proliferation and angiogenesis.
      Minami T, Horiuchi K, Miura M, Abid MR, Takabe W, Noguchi N, Kohro T, Ge X, Aburatani H, Hamakubo T, Kodama T, Aird WC.
      J Biol Chem. 2004 Nov 26;279(48):50537-54. PMID: 15448146
      血管内皮細胞のサンドイッチ培養。
    • Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture.
      Kikuchi H, Suzuki K, Sakai N, Yamada S.
      Cell Tissue Res. 2004 Aug;317(2):173-85. PMID: 15221440
      歯乳頭細胞のサンドイッチ培養。
    • Vascular endothelial growth factor- and thrombin-induced termination factor, Down syndrome critical region-1, attenuates endothelial cell proliferation and angiogenesis.
      Minami T, Horiuchi K, Miura M, Abid MR, Takabe W, Noguchi N, Kohro T, Ge X, Aburatani H, Hamakubo T, Kodama T, Aird WC.
      J Biol Chem. 2004 Nov 26;279(48):50537-54. PMID: 15448146
      血管内皮細胞のサンドイッチ培養。
    • Interaction between hex and GATA transcription factors in vascular endothelial cells inhibits flk-1/KDR-mediated vascular endothelial growth factor signaling.
      Minami T, Murakami T, Horiuchi K, Miura M, Noguchi T, Miyazaki J, Hamakubo T, Aird WC, Kodama T.
      J Biol Chem. 2004 May 14;279(20):20626-35. PMID: 15016828
      血管内皮細胞のサンドイッチ培養。
    • Crosslinking of collagen gels by transglutaminase.
      Orban JM, Wilson LB, Kofroth JA, El-Kurdi MS, Maul TM, Vorp DA.
      J Biomed Mater Res A. 2004 Mar 15;68(4):756-62. PMID: 14986330
      骨髄由来間質細胞を架橋度の異なるコラーゲンゲル内で三次元培養。
    • Heterologous desensitization of T cell functions by CCR5 and CXCR4 ligands: inhibition of cellular signaling, adhesion and chemotaxis.
      Hecht I, Cahalon L, Hershkoviz R, Lahat A, Franitza S, Lider O.
      Int Immunol. 2003 Jan;15(1):29-38. PMID: 12502723
      T細胞をコラーゲンを含む基底膜成分混合ゲル内で三次元培養。
    • Collapsin response mediator protein-2 accelerates axon regeneration of nerve-injured motor neurons of rat.
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      J Neurochem. 2003 Aug;86(4):1042-50. PMID: 12887701
      後根神経節(DRG)のコラーゲンゲル内培養。
    • Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen I gels.
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      軟骨細胞のコラーゲンゲル内三次元培養。
    • Growth, antrum formation, and estradiol production of bovine preantral follicles cultured in a serum-free medium.
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      Biol Reprod. 2002 Oct;67(4):1099-105. PMID: 12297524
      前胞状卵胞のコラーゲンゲル内培養。
    • Tea catechins inhibit angiogenesis in vitro, measured by human endothelial cell growth, migration and tube formation, through inhibition of VEGF receptor binding.
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      Cancer Lett. 2002 Jun 28;180(2):139-44. PMID: 12175544
      血管内皮細胞のサンドイッチ培養。
    • beta4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium.
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      Cancer Cell. 2002 Sep;2(3):205-16. PMID: 12242153
      乳腺上皮細胞のゲル内三次元培養。
    • Resveratrol and quercetin inhibit angiogenesis in vitro.
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      Cancer Lett. 2001 Sep 28;171(1):11-6. PMID: 11485823
      大動脈内皮細胞のコラーゲンゲル内三次元培養。
    • The interplay of matrix metalloproteinases, morphogens and growth factors is necessary for branching of mammary epithelial cells.
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      乳腺上皮細胞のコラーゲンゲル内三次元培養や乳腺のコラーゲンゲル内器官培養。
    • Concept for organ engineering: a reconstruction method of rat liver for in vitro culture.
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      肝葉をコラーゲンゲル上、コラーゲンゲルサンドイッチ培養。肝臓摘出前の還流液にも使用。
    • The small GTP-binding protein TC10 promotes nerve elongation in neuronal cells, and its expression is induced during nerve regeneration in rats.
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      後根神経節(DRG)のコラーゲンゲル内培養。
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      乳腺上皮細胞のコラーゲンゲル内三次元培養。コラーゲンコーティングも。
    • Tissue phenotype depends on reciprocal interactions between the extracellular matrix and the structural organization of the nucleus.
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    • Trophoblast stem cell-based organoid models of the human placental barrier.
      Hori T, Okae H, Shibata S, Kobayashi N, Kobayashi EH, Oike A, Sekiya A, Arima T, Kaji H.
      Nat Commun. 2024 Feb 8;15(1):962. PMID: 38332125.
      コラーゲンビトリゲルをコラーゲンコーティングし、栄養膜幹細胞を培養。
    • JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes.
      Ogawa S, Ishii T, Otani T, Inai Y, Matsuura T, Inai T.
      Histochem Cell Biol. 2024 Jan 16. doi: 10.1007/s00418-023-02264-8. Epub ahead of print. PMID: 38227055.
    • Pivotal role of IL-8 derived from the interaction between osteosarcoma and tumor-associated macrophages in osteosarcoma growth and metastasis via the FAK pathway.
      Tatsuno R, Ichikawa J, Komohara Y, Pan C, Kawasaki T, Enomoto A, Aoki K, Hayakawa K, Iwata S, Jubashi T, Haro H.
      Cell Death Dis. 2024 Feb 1;15(2):108. PMID: 38302407.
      セルカルチャーインサートをコラーゲンコーティングし、骨肉腫細胞の浸潤を評価。
    • ECM proteins and cationic polymers coating promote dedifferentiation of patient-derived mature adipocytes to stem cells.
      Karanfil AS, Louis F, Sowa Y, Matsusaki M.
      Biomater Sci. 2023 Nov 21;11(23):7623-7638. PMID: 37830400.
    • An in vitro culture platform to study the extracellular matrix remodeling potential of human mesenchymal stem cells.
      Kim MH, Tan SY, Yamahara K, Kino-Oka M.
      Acta Biomater. 2023 Oct 15;170:376-388. PMID: 37619896.
      フラスコをコーティングし、間葉系幹細胞を培養。
    • Mechanopathology of biofilm-like Mycobacterium tuberculosis cords.
      Mishra R, Hannebelle M, Patil VP, Dubois A, Garcia-Mouton C, Kirsch GM, Jan M, Sharma K, Guex N, Sordet-Dessimoz J, Perez-Gil J, Prakash M, Knott GW, Dhar N, McKinney JD, Thacker VV.
      Cell. 2023 Nov 9;186(23):5135-5150.e28. PMID: 37865090.
      Lung-on-chipデバイスをコラーゲンコーティングし、結核菌の感染モデルを作製。
    • Changes in the intra- and extra-mechanical environment of the nucleus in Saos-2 osteoblastic cells during bone differentiation process: Nuclear shrinkage and stiffening in cell differentiation.
      Nagayama K, Kodama F, Wataya N, Sato A, Matsumoto T.
      J Mech Behav Biomed Mater. 2023 Feb;138:105630 PMID: 36565693.
      ガラスボトムディッシュをコラーゲンコーティングし、骨芽細胞を培養。
    • Mechanoregulation of Osteoclastogenesis-Inducing Potentials of Fibrosarcoma Cell Line by Substrate Stiffness.
      Tiskratok W, Yamada M, Watanabe J, Pengyu Q, Kimura T, Egusa H.
      Int J Mol Sci. 2023 May 18;24(10):8959. PMID: 37240303.
      プレートや強度の異なるPDMS基材をコラーゲンコーティングし、線維芽細胞やマクロファージ様細胞を培養。
    • Substrate stiffness controls proinflammatory responses in human gingival fibroblasts.
      Tiskratok W, Yamada M, Watanabe J, Kartikasari N, Kimura T, Egusa H.
      Sci Rep. 2023 Jan 24;13(1):1358. PMID: 36693942.
      プレートや強度の異なるPDMS基材をコラーゲンコーティングし、歯肉線維芽細胞を培養。
    • The cGAS-STING pathway drives type I IFN immunopathology in COVID-19.
      Domizio JD, Gulen MF, Saidoune F, Thacker VV, Yatim A, Sharma K, Nass T, Guenova E, Schaller M, Conrad C, Goepfert C, de Leval L, Garnier CV, Berezowska S, Dubois A, Gilliet M, Ablasser A.
      Nature. 2022 Mar;603(7899):145-151. PMID: 35045565.
      Lung-on-chipデバイスをコラーゲンコーティングし、SARS-CoV-2の感染モデルを作製。
    • VEGF-A promotes the motility of human melanoma cells through the VEGFR1-PI3K/Akt signaling pathway.
      Koizumi K, Shintani T, Hayashido Y, Hamada A, Higaki M, Yoshioka Y, Sakamoto A, Yanamoto S, Okamoto T.
      In Vitro Cell Dev Biol Anim. 2022 Sep;58(8):758-770. PMID: 35997849; PMCID: PMC9550759.
      プレートをコラーゲンコーティングし、メラノーマ細胞を培養。
    • Characterization of morphological alterations in micropapillary adenocarcinoma of the lung using an established cell line.
      Sonoda D, Kamizaki K, Matsuo Y, Aruga K, Mikubo M, Yamashita K, Nishita M, Minami Y, Satoh Y.
      Oncol Rep. 2022 Jan;47(1):19. PMID: 34796907.
      カバースリップをコラーゲンコーティングし、肺がん細胞を培養。
    • Tenogenic Induction From Induced Pluripotent Stem Cells Unveils the Trajectory Towards Tenocyte Differentiation.
      Yoshimoto Y, Uezumi A, Ikemoto-Uezumi M, Tanaka K, Yu X, Kurosawa T, Yambe S, Maehara K, Ohkawa Y, Sotomaru Y, Shukunami C.
      Front Cell Dev Biol. 2022 Mar 9;10:780038. PMID: 35372337.
      ディッシュをコラーゲンコーティングし、マウス尾腱を培養。
    • A candidate gene of Alzheimer diseases was mutated in senescence-accelerated mouse prone (SAMP) 8 mice.
      Akbor MM, Kim J, Nomura M, Sugioka J, Kurosawa N, Isobe M.
      Biochem Biophys Res Commun. 2021 Aug 4;572:112-117. PMID: 34364289.
      カバーガラスをコラーゲンコーティングし、腎細胞を培養。
    • Essential Role of Extracellular Type V Collagen for Porcine Adipocyte Development.
      Ikuyo Nakajima, Fumio Nakamura, Koichi Chikuni.
      Jpn. J. Swine Science, 58, 2: 53-64.
      ディッシュをコラーゲンコーティングし、皮下脂肪前駆細胞を培養。
    • Physical Activity Attenuates the Obesity-Induced Dysregulated Expression of Brown Adipokines in Murine Interscapular Brown Adipose Tissue.
      Sakurai T, Fukutomi T, Yamamoto S, Nozaki E, Kizaki T.
      Int J Mol Sci. 2021 Sep 27;22(19):10391. PMID: 34638731.
      プレートをコラーゲンコーティングし、褐色脂肪細胞を培養。
    • Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection.
      Sharma K, Dhar N, Thacker VV, Simonet TM, Signorino-Gelo F, Knott GW, McKinney JD.
      Elife. 2021 Jul 5;10:e66481. PMID: 34219648
      マイクロ流体デバイスをコラーゲンコーティングし、膀胱上皮細胞と膀胱微小血管内皮細胞を共培養。
    • Rapid endotheliitis and vascular damage characterize SARS-CoV-2 infection in a human lung-on-chip model.
      Thacker VV, Sharma K, Dhar N, Mancini GF, Sordet-Dessimoz J, McKinney JD.
      EMBO Rep. 2021 Jun 4;22(6):e52744. PMID: 33908688.
      Lung-on-chipデバイスをコラーゲンコーティングし、SARS-CoV-2の感染モデルを作製。
    • Disturbance of hepatocyte growth and metabolism in a hyperammonemia microenvironment.
      Wang Q, Guan K, Lv Y, Zhang Y, Yu Z, Kan Q.
      Arch Biochem Biophys. 2022 Feb 15;716:109109. PMID: 34932992.
      プレートをコラーゲンコーティングし、肝細胞を培養。
    • Three-Dimensional Cell Sheet Construction Method with a Polyester Micromesh Sheet.
      Hori T, Kurosawa O, Ishihara K, Mizuta T, Iwata H.
      Tissue Eng Part C Methods. 2020 Mar;26(3):170-179. PMID: 32186996
      メッシュをコラーゲンコーティングし、線維芽細胞や肝細胞、間葉系幹細胞を培養。
    • The reduced susceptibility of mouse keratinocytes to retinoic acid may be involved in the keratinization of oral and esophageal mucosal epithelium.
      Miyazono S, Otani T, Ogata K, Kitagawa N, Iida H, Inai Y, Matsuura T, Inai T.
      Histochem Cell Biol. 2020 Apr;153(4):225-237. PMID: 32006103
    • A lung-on-chip model of early Mycobacterium tuberculosis infection reveals an essential role for alveolar epithelial cells in controlling bacterial growth.
      Thacker VV, Dhar N, Sharma K, Barrile R, Karalis K, McKinney JD.
      Elife. 2020 Nov 24;9:e59961. PMID: 33228849.
      マイクロ流体デバイスをコラーゲンコーティングし、肺胞上皮細胞を培養。
    • CCL2 secreted from cancer-associated mesothelial cells promotes peritoneal metastasis of ovarian cancer cells through the P38-MAPK pathway.
      Yasui H1,2, Kajiyama H3, Tamauchi S1, Suzuki S1, Peng Y1, Yoshikawa N1, Sugiyama M1, Nakamura K1, Kikkawa F1.
      Clin Exp Metastasis. 2020 Feb;37(1):145-158. PMID: 31541326
      カバーグラスをコラーゲンコーティングし、腹膜中皮細胞を培養。
    • Filopodia play an important role in the trans-mesothelial migration of ovarian cancer cells.
      Yoshihara M, Yamakita Y, Kajiyama H, Senga T, Koya Y, Yamashita M, Nawa A, Kikkawa F.
      Exp Cell Res. 2020 Jul 15;392(2):112011. PMID: 32339607
      カバーガラスをコラーゲンコーティングし、中皮細胞を培養。中皮細胞層の上に卵巣がん細胞を播種して浸潤を評価。
    • The novel lncRNA CALIC upregulates AXL to promote colon cancer metastasis.
      Kawasaki Y, Miyamoto M, Oda T, Matsumura K, Negishi L, Nakato R, Suda S, Yokota N, Shirahige K, Akiyama T.
      EMBO Rep. 2019 Aug;20(8):e47052. PMID: 31353791; PMCID: PMC6680119.
      セルカルチャーインサートをコラーゲンコーティングし、大腸がん細胞の浸潤を評価。
    • Biomechanical analysis of the mechanical environment of the cell nucleus in serum starvation-induced vascular smooth muscle cell differentiation.
      Kazuaki NAGAYAMA.
      Journal of Biomechanical Science and Engineering, Vol.14, No.4 (2019)
      ディッシュをコラーゲンコーティングし、血管平滑筋細胞を培養。
    • Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation.
      Takimoto A, Kokubu C, Watanabe H, Sakuma T, Yamamoto T, Kondoh G, Hiraki Y, Shukunami C.
      Sci Rep. 2019 Mar 14;9(1):4605. PMID: 30872687
      ディッシュをコラーゲンコーティングし、線維輪組織由来細胞を培養。
    • Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.
      Ueda M, Sato Y, Horie A, Tani H, Miyazaki Y, Okunomiya A, Matsumoto H, Hamanishi J, Kondoh E, Mandai M.
      Mol Cell Endocrinol. 2019 Apr 11. pii: S0303-7207(19)30106-6. PMID: 30981734
      セルカルチャーインサートをコラーゲンコーティングし、絨毛外栄養膜細胞を培養。
    • Reciprocal expression of Slug and Snail in human oral cancer cells.
      Nakamura R, Ishii H, Endo K, Hotta A, Fujii E, Miyazawa K, Saitoh M.
      PLoS One. 2018 Jul 3;13(7):e0199442. PMID: 29969465
      セルカルチャーインサートをコラーゲンコーティングし、口腔扁平上皮がん細胞を培養し、浸潤評価。
    • MicroRNA 148a-3p promotes Thrombospondin-4 expression and enhances angiogenesis during tendinopathy development by inhibiting Krüppel-like factor 6.
      Ge H, Shrestha A, Liu C, Wu P, Cheng B.
      Biochem Biophys Res Commun. 2018 Jul 12;502(2):276-282. PMID: 29807011
      培養器材をコラーゲンコーティングし、腱細胞を培養。
    • Internalization of CD239 highly expressed in breast cancer cells: a potential antigen for antibody-drug conjugates.
      Kikkawa Y, Enomoto-Okawa Y, Fujiyama A, Fukuhara T, Harashima N, Sugawara Y, Negishi Y, Katagiri F, Hozumi K, Nomizu M, Ito Y.
      Sci Rep. 2018 Apr 26;8(1):6612. PMID: 29700410.
      ディッシュをコーティングし、血管内皮細胞を培養。
    • Enhanced survival and insulin secretion of insulinoma cell aggregates by incorporating gelatin hydrogel microspheres.
      Kanako Inoo, Hiroto Bando, Yasuhiko Tabata.
      Regenerative Therapy, Volume 8, June 2018, Pages 29-37.
      プレートをコラーゲンコーティングし、膵臓β細胞を培養。
    • Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes.
      Shukunami C, Takimoto A, Nishizaki Y, Yoshimoto Y, Tanaka S, Miura S, Watanabe H, Sakuma T, Yamamoto T, Kondoh G, Hiraki Y.
      Sci Rep. 2018 Feb 16;8(1):3155. PMID: 29453333
      ディッシュをコラーゲンコーティングし、腱細胞を培養。
    • 肺胞上皮細胞および血管内皮細胞遊走能への肺線維芽細胞共培養の影響
      高橋君子
      鎌倉女子大学紀要 巻25 ページ 17 - 26 発行年 2018-01
      セルカルチャーインサートをコラーゲンコーティングし、肺胞上皮細胞を培養し、細胞遊走評価。
    • Actomyosin contractility provokes contact inhibition in E-cadherin-ligated keratinocytes.
      Hirata H, Samsonov M, Sokabe M.
      Sci Rep. 2017 Apr 13;7:46326. PMID: 28406163
      プレートやガラスボトムディッシュをコラーゲンコーティングし、角化細胞や乳腺上皮細胞を培養。
    • Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes.
      Kriszt R, Arai S, Itoh H, Lee MH, Goralczyk AG, Ang XM, Cypess AM, White AP, Shamsi F, Xue R, Lee JY, Lee SC, Hou Y, Kitaguchi T, Sudhaharan T, Ishiwata S, Lane EB, Chang YT, Tseng YH, Suzuki M, Raghunath M.
      Sci Rep. 2017 May 3;7(1):1383. PMID: 28469146
      マイクロカロリメーター用のプレートをコラーゲンコーティングし、褐色脂肪前駆細胞を培養。
    • The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration.
      Matsumura K, Kawasaki Y, Miyamoto M, Kamoshida Y, Nakamura J, Negishi L, Suda S, Akiyama T.
      Oncogene. 2017 Mar 2;36(9):1191-1199. PMID: 27797375
      プレートをコラーゲンコーティングし、結腸がん細胞を培養。
    • Bio-active molecules modified surfaces enhanced mesenchymal stem cell adhesion and proliferation.
      Mobasseri R, Tian L, Soleimani M, Ramakrishna S, Naderi-Manesh H.
      Biochem Biophys Res Commun. 2017 Jan 29;483(1):312-317. PMID: 28025144
      カバースリップをコラーゲンコーティングし、骨髄由来間葉系幹細胞を培養。
    • Methanol and Butanol Extracts of Paeonia lutea Leaves Repress Metastasis of Squamous Cell Carcinoma.
      Mukudai Y, Zhang M, Shiogama S, Kondo S, Ito C, Motohashi H, Kato K, Fujii M, Shintani S, Shigemori H, Yazawa K, Shirota T.
      Evid Based Complement Alternat Med. 2016;2016:6087213. PMID: 27293462
      セルカルチャーインサートをコラーゲンコーティングし、口腔扁平上皮がん細胞を培養。
    • Chronic inflammation imposes aberrant cell fate in regenerating epithelia through mechanotransduction.
      Nowell CS, Odermatt PD, Azzolin L, Hohnel S, Wagner EF, Fantner GE, Lutolf MP, Barrandon Y, Piccolo S, Radtke F.
      Nat Cell Biol. 2016 Feb;18(2):168-80. PMID: 26689676
      カバースリップをコーティングし、角膜上皮細胞を培養。
    • Ammonia-induced energy disorders interfere with bilirubin metabolism in hepatocytes.
      Wang Q, Wang Y, Yu Z, Li D, Jia B, Li J, Guan K, Zhou Y, Chen Y, Kan Q.
      Arch Biochem Biophys. 2014 Aug;555-556:16-22. PMID: 24878366
      プレートをコラーゲンコーティングし、肝細胞を培養。
    • Isolation of mesenchymal stem cells from human dermis.
      Soma T, Kishimoto J, Fisher D.
      Methods Mol Biol. 2013;989:265-74. PMID: 23483401
      チャンバースライドをコラーゲンコーティングし、皮膚由来間質細胞を培養。
    • Dexamethasone-mediated transcriptional regulation of rat carboxylesterase 2 gene.
      Hori T, Jin L, Fujii A, Furihata T, Nagahara Y, Chiba K, Hosokawa M.
      Xenobiotica. 2012 Jul;42(7):614-23. PMID:22235919
      プレートをコラーゲンコーティングし、肝細胞を培養。
    • Fibroblast Growth Factor Receptor 2 IIIc as a Therapeutic Target for Colorectal Cancer Cells.
      Matsuda Y, Hagio M, Seya T, Ishiwata T.
      Mol Cancer Ther. 2012 Sep;11(9):2010-20. PMID:22778155
      プレートをコラーゲンコーティングし、大腸がん細胞を培養。
    • Effect of preservation conditions of collagen substrate on its fibril formation and rabbit chondrocyte morphology.
      Nadzir MM, Kino-oka M, Sugawara K, Taya M.
      J Biosci Bioeng. 2012 Sep;114(3):360-3. PMID: 22677065
      保存条件の異なるコラーゲン溶液を用いてフラスコをコーティングし、軟骨細胞を培養。
    • Grainyhead-like 2 regulates epithelial morphogenesis by establishing functional tight junctions through the organization of a molecular network among claudin3, claudin4, and Rab25.
      Senga K, Mostov KE, Mitaka T, Miyajima A, Tanimizu N.
      Mol Biol Cell. 2012 Aug;23(15):2845-55. PMID:22696678
      プレートにコラーゲン+マトリゲルをコーティングし、肝前駆細胞を培養。
    • α1- and α5-containing Laminins Regulate the Development of Bile Ducts via β1 Integrin Signals.
      Tanimizu N, Kikkawa Y, Mitaka T, Miyajima A.
      J Biol Chem. 2012 Aug 17;287(34):28586-97. PMID:22761447
      ガラスチャンバーにコラーゲン+マトリゲルをコーティングし、肝前駆細胞を培養。
    • Modulation of cytochrome P450 gene expression in primary hepatocytes on various artificial extracellular matrices.
      Adachi T, Goto M, Cho CS, Akaike T.
      Biochem Biophys Res Commun. 2011 Oct 7;413(4):577-81. PMID: 21930114
      プレートをコラーゲンコーティングし、肝細胞を培養。
    • Overexpressed fibroblast growth factor receptor 2 in the invasive front of colorectal cancer: a potential therapeutic target in colorectal cancer.
      Matsuda Y, Ishiwata T, Yamahatsu K, Kawahara K, Hagio M, Peng WX, Yamamoto T, Nakazawa N, Seya T, Ohaki Y, Naito Z.
      Cancer Lett. 2011 Oct 28;309(2):209-19. PMID: 21745712
      プレートをコラーゲンコーティングし、大腸がん細胞を培養。
    • Comprehension of terminal differentiation and dedifferentiation of chondrocytes during passage cultures.
      Nadzir MM, Kino-oka M, Maruyama N, Sato Y, Kim MH, Sugawara K, Taya M.
      J Biosci Bioeng. 2011 Oct;112(4):395-401. PMID: 21778110
      フラスコをコラーゲンコーティングし、軟骨細胞を培養。アテロコラーゲンを用いたコラーゲンゲル内培養も。
    • Bile salt export pump inhibitors are associated with bile acid-dependent drug-induced toxicity in sandwich-cultured hepatocytes.
      Ogimura E, Sekine S, Horie T.
      Biochem Biophys Res Commun. 2011 Dec 16;416(3-4):313-7. PMID: 22108051
      プレートをコラーゲンコーティングし、肝細胞を培養。
    • Combined stimulation with cyclic stretching and hypoxia increases production of matrix metalloproteinase-9 and cytokines by macrophages.
      Oya K, Sakamoto N, Ohashi T, Sato M.
      Biochem Biophys Res Commun. 2011 Sep 9;412(4):678-82. PMID: 21867689
      シリコンチャンバーをコーティングし、血管平滑筋細胞を伸展培養。
    • Cell behavior on protein matrices containing laminin α1 peptide AG73.
      Yamada Y, Katagiri F, Hozumi K, Kikkawa Y, Nomizu M.
      Biomaterials. 2011 Jul;32(19):4327-35. PMID: 21420730
      プレートをコラーゲン+syndecan-binding peptides でコーティングし、褐色細胞腫由来細胞を培養。
    • Enhanced expression of lumican inhibited the attachment and growth of human embryonic kidney 293 cells.
      Ishiwata T, Yamamoto T, Kawahara K, Kawamoto Y, Matsuda Y, Ishiwata S, Naito Z.
      Exp Mol Pathol. 2010 Jun;88(3):363-70. PMID: 20138170
      プレートをコラーゲンコーティングし、腎細胞を培養。
    • Are integrin alpha(2)beta(1), glycoprotein Ib and vWf levels correlated with their contributions to platelet adhesion on collagen under high-shear flow?
      Jung SM, Sonoda M, Tsuji K, Jimi A, Nomura S, Kanaji T, Moroi M.
      Platelets. 2010;21(2):101-11. PMID: 20063990
      スライドガラスをコラーゲンコーティングし、血小板付着を評価。
    • The adenomatous polyposis coli-associated guanine nucleotide exchange factor Asef is involved in angiogenesis.
      Kawasaki Y, Jigami T, Furukawa S, Sagara M, Echizen K, Shibata Y, Sato R, Akiyama T.
      J Biol Chem. 2010 Jan 8;285(2):1199-207. PMID: 19897489
      セルカルチャーインサートをコラーゲンコーティングし、血管内皮細胞を培養。
    • Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film.
      Nishiyama H, Suga M, Ogura T, Maruyama Y, Koizumi M, Mio K, Kitamura S, Sato C.
      J Struct Biol. 2010 Nov;172(2):191-202. PMID: 20723603
      ディッシュをコラーゲンコーティングし、褐色細胞腫由来細胞を培養。
    • Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes.
      Sasai N, Agata N, Inoue-Miyazu M, Kawakami K, Kobayashi K, Sokabe M, Hayakawa K.
      Muscle Nerve. 2010 Jan;41(1):100-6. PMID: 19768770
      ディッシュをコラーゲンコーティングし、筋芽細胞を培養。
    • Expression of Notch signalling-related genes in normal and differentiating rat dental pulp cells.
      Sun H, Kawashima N, Xu J, Takahashi S, Suda H.
      Aust Endod J. 2010 Aug;36(2):54-8. PMID: 20666749
      ディッシュをコラーゲンコーティングし、歯髄細胞を培養。
    • Natalizumab has no direct biological effect on JC virus infectivity in permissive human neural cell lines.
      Suzuki T, Yamanouchi S, Sunden Y, Orba Y, Kimura T, Sawa H.
      J Med Virol. 2010 Jul;82(7):1229-35. PMID: 20513089
      ディッシュをコラーゲンコーティングし、神経細胞を培養。
    • Construction of multifunctional proteins for tissue engineering: epidermal growth factor with collagen binding and cell adhesive activities.
      Hannachi Imen E, Nakamura M, Mie M, Kobatake E.
      J Biotechnol. 2009 Jan 1;139(1):19-25. PMID: 18984018
      プレートをコラーゲンコーティング。さらにコラーゲン結合ドメイン+ERE+EGF融合タンパク質を添加し、肺胞基底上皮腺がん細胞を培養。
    • Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase.
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    • A collagen-coated surface enables quantitative evaluation of morphological behaviors of rabbit chondrocytes relating to cell differentiation in an early culture phase.
      Ali Baradar Khoshfetrata, Masahiro Kino-okaa, Katsura Sugawarab and Masahito Taya
      Biochemical Engineering Journal Volume 45, Issue 1, 1 June 2009, Pages 60-68
      プレートをコラーゲンコーティングし、軟骨細胞を培養。
    • ATP Release from Cultured Endothelial Cells and Intercellular Calcium Signaling during Shear Stress Exposure.
      Kudo Susumu, Hosoe Kaoru, Hosobuchi Makoto, Kawasaki Naoto, Tanishita Kazuo
      Journal of Biomechanical Science and Engineering 4(2), 274-285, 2009.
      ガラスボトムディッシュをコラーゲンコーティングし、血管内皮細胞を培養。
    • Human mammary progenitor cell fate decisions are products of interactions with combinatorial microenvironments.
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    • Contribution of the LIM domain and nebulin-repeats to the interaction of Lasp-2 with actin filaments and focal adhesions.
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    • Asef2 and Neurabin2 cooperatively regulate actin cytoskeletal organization and are involved in HGF-induced cell migration.
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    • Detection of mRNA in single living cells using AFM nanoprobes.
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    • Expansion and characterization of adipose tissue-derived stromal cells cultured with low serum medium.
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    • Different regulation of hepatocyte behaviors between natural extracellular matrices and synthetic extracellular matrices by hepatocyte growth factor.
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      プレートをコラーゲンコーティングし、肝細胞を培養。
    • Ectopic transplantation of hepatocyte sheets fabricated with temperature-responsive culture dishes.
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      温度応答性細胞培養皿をコラーゲンコーティングし、肝細胞を培養。
    • Effect of dehydroaltenusin-C12 derivative, a selective DNA polymerase alpha inhibitor, on DNA replication in cultured cells.
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    • Primary hepatocyte survival on non-integrin-recognizable matrices without the activation of Akt signaling.
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      Biomaterials. 2007 Feb;28(6):1093-104. PMID: 17081603
      ディッシュやプレートをコラーゲンコーティングし、肝細胞を培養。
    • Epidermal growth factor signaling for matrix-dependent cell proliferation and differentiation in primary cultured hepatocytes.
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    • Comparison of basal gene expression and induction of CYP3As in HepG2 and human fetal liver cells.
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      Biol Pharm Bull. 2007 Nov;30(11):2091-7. PMID: 17978482
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    • A mechanism to safeguard platelet adhesion under high shear flow: von Willebrand factor-glycoprotein Ib and integrin alphabeta-collagen interactions make complementary, collagen-type-specific contributions to adhesion.
      Moroi M, Jung SM.
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    • Combinatorial protein display for the cell-based screening of biomaterials that direct neural stem cell differentiation.
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    • Identification and characterization of GSRP-56, a novel Golgi-localized spectrin repeat-containing protein.
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    • Murine metanephric mesenchyme possesses characteristics of vascular endothelial cells in vitro.
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    • Association of expression of receptor for advanced glycation end products and invasive activity of oral squamous cell carcinoma.
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    • Fibrous component of the blastocoelic extracellular matrix shapes epithelia in concert with mesenchyme cells in starfish embryos.
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    • Process design of chondrocyte cultures with monolayer growth for cell expansion and subsequent three-dimensional growth for production of cultured cartilage.
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    • Role of p38 mitogen-activated protein kinase activation in podocyte injury and proteinuria in experimental nephrotic syndrome.
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    • Effect of fluid force on vascular cell function.
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      カバースリップをコラーゲンコーティングし、血管内皮細胞を培養。
    • Significance of HDAC6 regulation via estrogen signaling for cell motility and prognosis in estrogen receptor-positive breast cancer.
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      セルカルチャーインサートをコラーゲンコーティングし、乳がん細胞を培養。
    • Platelet-derived soluble factors induce human extravillous trophoblast migration and differentiation: platelets are a possible regulator of trophoblast infiltration into maternal spiral arteries.
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    • Regulation of biological activity and matrix assembly of laminin-5 by COOH-terminal, LG4-5 domain of alpha3 chain.
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    • Genotype 2a hepatitis C virus subgenomic replicon can replicate in HepG2 and IMY-N9 cells.
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    • Astrocytes express type VIII collagen during the repair process of brain cold injury.
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    • Expression of lysosomal protective protein/cathepsin A in a stably transformed human neuroblastoma cell line during bi-directional differentiation into neuronal and Schwannian cells.
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    • N-WASP and WAVE2 acting downstream of phosphatidylinositol 3-kinase are required for myogenic cell migration induced by hepatocyte growth factor.
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    • Microbial serine carboxypeptidase inhibitors--comparative analysis of actions on homologous enzymes derived from man, yeast and wheat.
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    • The Use of a Dehydrated Cellularized Collagen Matrix to Replace Fibrotic Vocal Fold Mucosa.
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    • Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models.
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    • A Compressed Collagen Construct for Studying Endothelial-Smooth Muscle Cell Interaction Under High Shear Stress.
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    • Flexibly Deformable Collagen Hydrogel Tube Reproducing Immunological Tissue Deformation of Blood Vessels as a Pharmacokinetic Testing Model.
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    • Rapid fabrication of tendon-like collagen gel via simultaneous fibre alignment and intermolecular cross-linking under mechanical loading.
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    • In vitro proliferation and long-term preservation of functional primary rat hepatocytes in cell fibers.
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    • Bi-layered carboxymethyl cellulose-collagen vitrigel dual-surface adhesion-prevention membrane.
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    • Titanium Nanosurface with a Biomimetic Physical Microenvironment to Induce Endogenous Regeneration of the Periodontium.
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    • A Loss of Nuclear-Cytoskeletal Interactions in Vascular Smooth Muscle Cell Differentiation Induced by a Micro-Grooved Collagen Substrate Enabling the Modeling of an In Vivo Cell Arrangement.
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    • A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue.
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    • Polyanion-induced, microfluidic engineering of fragmented collagen microfibers for reconstituting extracellular environments of 3D hepatocyte culture.
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      コラーゲンファイバーを断片化したコラーゲンマイクロファイバーを作製し、初代肝細胞をスフェロイド培養。
    • Short-Term Preclinical Application of Functional Human Induced Pluripotent Stem Cell-Derived Airway Epithelial Patches.
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      シルクフィブロインフィルムの上にコラーゲンゲルビトリゲルを作製し、その上で粘膜上皮を形成させ、ブタ気管欠損部へ移植。
    • Efficient Decellularization by Application of Moderate High Hydrostatic Pressure with Supercooling Pretreatment.
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      コラーゲンゲルに高静水圧をかけ、その後線維芽細胞をゲル内で培養。
    • PLGA-collagen-ECM hybrid meshes mimicking stepwise osteogenesis and their influence on the osteogenic differentiation of hMSCs.
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      PLGAとコラーゲンとキトサンからなるメッシュを作製し、間葉系幹細胞を培養。
    • Cell-encapsulated chitosan-collagen hydrogel hybrid nerve guidance conduit for peripheral nerve regeneration.
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      シュワン細胞を含むコラーゲンが内側、キトサンが外側の二層構造チューブを作製。
    • Homeostatic mini-intestines through scaffold-guided organoid morphogenesis.
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      コラーゲンとマトリゲルの混合ゲルをマイクロ流路デバイス中に作製し、腸オルガノイド由来細胞を播種してミニ腸を作製。
    • Coaxial printing of double-layered and free-standing blood vessel analogues without ultraviolet illumination for high-volume vascularised tissue. Biofabrication.
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      血管内皮細胞を含むコラーゲンが内側、血管肺活筋細胞を含むコラーゲン+アルギン酸ナトリウムが外側の二層構造細胞ファイバーを作製。
    • CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture
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      乳がん細胞や子宮頸がん細胞を含むコラーゲンスフェロイドを超疎水性多孔質モノリス上に作製。
    • Cell-laden microfibers fabricated using μL cell-suspension.
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      線維芽細胞を含むコラーゲンが内側、アルギン酸が外側の二層構造細胞ファイバーを作製し、筋芽細胞と共培養。
    • Novel microvascular endothelial model utilizing a collagen vitrigel membrane and its advantages for predicting histamine-induced microvascular hyperpermeability.
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      J Pharmacol Toxicol Methods. 2020 Sep 3;106:106916. PMID: 32890732.
      コラーゲンビトリゲルを作製し、微小血管内皮細胞単独、あるいは同細胞と線維芽細胞を共培養。
    • Millimeter-thick xenoislet-laden fibers as retrievable transplants mitigate foreign body reactions for long-term glycemic control in diabetic mice.
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      コラーゲン+筋芽細胞+マイクロキャリアからなる、ファイバー状のマイクロキャリア凝集塊「MC fibers」を作製。
    • Microfiber-shaped building-block tissues with endothelial networks for constructing macroscopic tissue assembly.
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      APL Bioeng. 2019 Nov 13;3(4):046101. PMID: 31737859
      肝細胞と血管内皮細胞を含むコラーゲンが内側、アルギン酸が外側の二層構造細胞ファイバーを作製。
    • A novel micro-grooved collagen substrate for inducing vascular smooth muscle differentiation through cell tissue arrangement and nucleus remodeling.
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      J Mech Behav Biomed Mater. 2019 Feb;90:295-305. PMID: 30396043
      微細溝を持つコラーゲンシートを作製し、血管平滑筋を培養。
    • Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip.
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      Biomed Microdevices. 2019 Dec 20;22(1):9. PMID: 31863202
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      Micromachines (Basel). 2019 May 29;10(6). PMID: 31146491
      脂肪細胞を含むコラーゲンが内側、アルギニン酸が外側の二層構造細胞ファイバーを作製。
    • VEGF delivery by smart polymeric PNIPAM nanoparticles affects both osteogenic and angiogenic capacities of human bone marrow stem cells.
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      VEGF+poly(N‑isopropylacrylamide) (PNIPAM) をコラーゲンゲルと混合し、骨髄由来間葉系幹細胞を培養。
    • Double-layer perfusable collagen microtube device for heterogeneous cell culture.
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      Biofabrication. 2018 Nov 30;11(1):015010. PMID: 30499456
    • An allogeneic 'off the shelf' therapeutic strategy for peripheral nerve tissue engineering using clinical grade human neural stem cells.
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      神経幹細胞含有Real Architecture for 3-Dimensional Tissue (RAFT)を作製後、円柱状に丸めてコラーゲンチューブに詰め、ラット坐骨神経切断モデルへ移植。
    • Optimal biomaterials for tracheal epithelial grafts: an in vitro systematic comparative analysis.
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      Acta Biomater. 2018 Nov;81:146-157. PMID: 30268918
      コラーゲンビトリゲル+シルクフィブロインスキャフォールドを作製。
    • The Effect of Hypothermic and Cryogenic Preservation on Engineered Neural Tissue.
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      Tissue Eng Part C Methods. 2017 Oct;23(10):575-582. PMID: 28877649
      シュワン細胞をReal Architecture for 3-Dimensional Tissue (RAFT)上で培養。
    • Functionalized core-shell hydrogel microsprings by anisotropic gelation with bevel-tip capillary.
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      肝がん細胞を含むコラーゲンが内側、アルギン酸が外側の二層構造細胞スプリングを作製。
    • Compartmentalized Spherical Collagen Microparticles for Anisotropic Cell Culture Microenvironments.
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      Adv Healthc Mater. 2017 Apr;6(8). PMID: 28322015
      半球ごとにECMの異なる細胞含有ビーズを作製。
    • Collagen Vitrigels with Low-fibril Density Enhance Human Embryonic Stem
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      コラーゲンビトリゲルを作製し、網膜色素上皮細胞を培養。
    • 3D Tissue Formation of Unilocular Adipocytes in Hydrogel Microfibers.
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      Adv Healthc Mater. 2016 Mar 9;5(5):548-56. PMID: 26680212
    • Influence of collagen source on fibrillar architecture and properties of vitrified collagen membranes.
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      ウシ由来コラーゲン、ヒト線維芽細胞由来コラーゲン、ヒト組み換えコラーゲンを用いてコラーゲンビトリゲルを作製。
    • An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings.
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      コラーゲンゲルフィルムを作製し、グリア細胞を培養。
    • Vessel-like channels supported by poly-l-lysine tubes.
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      J Biosci Bioeng. 2016 Dec;122(6):753-757 PMID: 27323931
      PLLチューブをコラーゲンゲル内に包埋し、血管用チャンネルを作製。
    • Formation of Highly Aligned Collagen Nanofibers by Continuous Cyclic Stretch of a Collagen Hydrogel Sheet.
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      Macromol Biosci. 2016 Jul;16(7):995-1000. PMID: 27136124
      配向性のあるコラーゲンシートを作製し、角膜間質細胞や平滑筋細胞を培養。
    • Regeneration of corneal epithelium utilizing a collagen vitrigel membrane in rabbit models for corneal stromal wound and limbal stem cell deficiency.
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      コラーゲンビトリゲルを作製し、ウサギ角膜間質創傷モデルや角膜輪部幹細胞欠損モデルへ移植。
    • Smooth muscle-like tissue constructs with circumferentially oriented cells formed by the cell fiber technology.
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      脱分化脂肪細胞+コラーゲン、あるいは脱分化脂肪細胞+コラーゲン+フィブリノゲンが内側、アルギン酸が外側の二層構造細胞ファイバーを作製。
    • Optimization of optical and mechanical properties of Real Architecture for 3-Dimensional Tissue equivalents: Towards treatment of limbal epithelial stem cell deficiency.
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      Acta Biomater. 2015 Sep 15;24:241-50. PMID: 26092352
      角膜輪部上皮細胞をReal Architecture for 3-Dimensional Tissue (RAFT)上で培養。
    • Fabrication of fibrillized collagen microspheres with the microstructure resembling an extracellular matrix.
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      Soft Matter. 2015 Apr 14;11(14):2844-51. PMID: 25708876
      線維化・非線維化コラーゲンミクロスフェアの作製。
    • Preparation Fibrillized Collagen-Glycosaminoglycan Complex Matrix Using Fibrillogenesis.
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      Macromol Symp. 2015 358:95–105.
      コラーゲン+ヒアルロン酸、コンドロイチン硫酸、ヘパリンスキャフォールドを作製。
    • Neural stem/progenitor cell-laden microfibers promote transplant survival in a mouse transected spinal cord injury model.
      Sugai K, Nishimura S, Kato-Negishi M, Onoe H, Iwanaga S, Toyama Y, Matsumoto M, Takeuchi S, Okano H, Nakamura M.
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      神経幹細胞/前駆細胞を含むコラーゲンが内側、アルギニン酸が外側の二層構造細胞ファイバーを作製し、マウス脊髄損傷部へ移植。
    • Activation of Yes-Associated Protein in Low-Grade Meningiomas Is Regulated by Merlin, Cell Density, and Extracellular Matrix Stiffness.
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      硬さの異なるアクリルアミドゲルをコラーゲンコーティングし、髄膜腫細胞を培養。
    • Application of a collagen-based membrane and chondroitin sulfate-based hydrogel adhesive for the potential repair of severe ocular surface injuries.
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      コラーゲンビトリゲルを作製し、ブタやウサギの眼に移植。
    • Design and construction of an equibiaxial cell stretching system that is improved for biochemical analysis.
      Ursekar CP, Teo SK, Hirata H, Harada I, Chiam KH, Sawada Y.
      PLoS One. 2014 Mar 13;9(3):e90665. PMID: 24626190
      PDMS膜をコラーゲンコーティングし、線維芽細胞や腎細胞を伸展培養。
    • Banded structures in collagen vitrigels for corneal injury repair.
      Xia Z, Calderón-Colón X, McCally R, Maranchi J, Rong L, Hsiao B, Elisseeff J, Trexler M.
      Acta Biomater. 2014 Aug;10(8):3615-9. PMID: 24859294
      コラーゲンビトリゲルを作製し、コラーゲン線維のバンド構造を評価。
    • Vitrified collagen-based conjunctival equivalent for ocular surface reconstruction.
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      Biomaterials. 2014 Aug;35(26):7398-406. PMID: 24933512
      コラーゲンビトリゲルを作製し、結膜上皮細胞を培養して結膜傷害モデルウサギへ移植。
    • Modulation of keratocyte phenotype by collagen fibril nanoarchitecture in membranes for corneal repair.
      Guo Q, Phillip JM, Majumdar S, Wu PH, Chen J, Calderón-Colón X, Schein O, Smith BJ, Trexler MM, Wirtz D, Elisseeff JH.
      Biomaterials. 2013 Dec;34(37):9365-72. PMID: 24041426
      コラーゲンビトリゲルを作製し、角膜実質細胞を培養。
    • Substrate stiffness regulates temporary NF-κB activation via actomyosin contractions.
      Ishihara S, Yasuda M, Harada I, Mizutani T, Kawabata K, Haga H.
      Exp Cell Res. 2013 Nov 15;319(19):2916-27. PMID: 24113574
      硬さの異なるアクリルアミドゲルをコラーゲンコーティングし、肺腺がん細胞を培養。
    • Metre-long cell-laden microfibres exhibit tissue morphologies and functions.
      Onoe H, Okitsu T, Itou A, Kato-Negishi M, Gojo R, Kiriya D, Sato K, Miura S, Iwanaga S, Kuribayashi-Shigetomi K, Matsunaga YT, Shimoyama Y, Takeuchi S
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      線維芽細胞、肝細胞、血管内皮細胞、子宮頸がん細胞、膵臓β細胞、筋芽細胞、心筋細胞、神経幹細胞、大脳皮質細胞をそれぞれ含むコラーゲンが内側、アルギン酸が外側の二層構造細胞ファイバーを作製。
    • Regenerative process of tracheal epithelium using a collagen vitrigel sponge scaffold.
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      Laryngoscope. 2013 Jun;123(6):1469-73. PMID: 23625522
      b-FGF含有コラーゲンビトリゲルスポンジを作製し、気管欠損モデルラットに移植。
    • Cellular response to substrate rigidity is governed by either stress or strain.
      Yip AK, Iwasaki K, Ursekar C, Machiyama H, Saxena M, Chen H, Harada I, Chiam KH, Sawada Y.
      Biophys J. 2013 Jan 8;104(1):19-29. PMID: 23332055
      硬さの異なるアクリルアミドゲルをコラーゲンコーティングし、線維芽細胞を培養。
    • Structure and properties of collagen vitrigel membranes for ocular repair and regeneration applications.
      Calderón-Colón X, Xia Z, Breidenich JL, Mulreany DG, Guo Q, Uy OM, Tiffany JE, Freund DE, McCally RL, Schein OD, Elisseeff JH, Trexler MM.
      Biomaterials. 2012 Nov;33(33):8286-95. PMID: 22920579
      コラーゲンビトリゲルを作製し、物性を評価。
    • Local mechanical stimulation of mardin-darby canine kidney cell sheets on temperature-responsive hydrogel.
      Harada I, Yanagisawa S, Iwasaki K, Cho CS, Akaike T.
      Int J Mol Sci. 2012;13(1):1095-108. PMID:22312306
      PNIPAAm/PAAmゲルをコラーゲンコーティングし、尿細管上皮細胞を培養。
    • Hybrid scaffolds composed of hyaluronic acid and collagen for cartilage regeneration.
      Hyun Jung Kim, Kab Keun Kim, Il Kyu Park, Baek Sun Choi, Jae Ho Kim and Moon Suk Kim
      Tissue Engineering and Regenerative Medicine. Volume 9, Number 2 (2012), 57-62.
      コラーゲン+ヒアルロン酸スポンジを作製し、軟骨細胞の培養やウサギ軟骨欠損部へ移植。
    • Fabrication of a heterostructural fibrillated collagen matrix for the regeneration of soft tissue function.
      Kwangwoo Nam, Yuuki Sakai, Yoshihide Hashimoto, Tsuyoshi Kimura and Akio Kishida
      Soft Matter, 2012, 8, 472-480.
      コラーゲンスポンジ/ゲルスキャフォールドを作製し、線維芽細胞を培養。
    • Matrix compliance and RhoA direct the differentiation of mammary progenitor cells.
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      アクリルアミドゲルをコラーゲンコーティングし、乳腺前駆細胞を培養。
    • Regulation of growth factors-associated cell migration by C-phycocyanin scaffold in dermal wound healing.
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      コラーゲン+PLLA+PGA+PLGA+C-フィコシアニンスキャフォールドを作製し、マウス創傷部へ移植。
    • Heterofunctional nanosheet controlling cell adhesion properties by collagen coating.
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      PLLAナノシートをコラーゲン溶液の添加、あるいはspin assembly法でコーティングし、線維芽細胞を培養。
    • Thermal denaturation of type I collagen vitrified gels.
      Zhiyong Xia, Xiomara Calderon-Colona, Morgana Trexlera, Jennifer Elisseeffb, Qiongyu Guob
      Thermochimica ActaVolume 527, 10 January 2012, Pages 172–179.
      コラーゲンビトリゲルを作製し、熱変性を評価。
    • Engineering a collagen matrix that replicates the biological properties of native extracellular matrix.
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      コラーゲンスキャフォールドを作製し、線維芽細胞の培養やマウス創傷部への移植。
    • A nano-fibrous assembly of collagen–hyaluronic acid for controlling cell-adhesive properties.
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      Soft Matter, 2010,6, 4672-4676
      酸化シリコンシートをコラーゲンコーティングし、線維芽細胞を培養。
    • Preparation of a collagen/polymer hybrid gel designed for tissue membranes. Part I: controlling the polymer-collagen cross-linking process using an ethanol/water co-solvent.
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      コラーゲン+MPCスキャフォールドの作製。
    • Preparation of a collagen/polymer hybrid gel for tissue membranes. Part II: in vitro and in vivo biological properties of the collagen gels.
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      コラーゲン+ポリマースキャフォールドを作製し、ラット皮下に移植。
    • Micro 3D Culture System using Hyaluronan-Collagen Capsule for Skeletal Muscle-Derived Stem Cells.
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      Open Tissue Eng Regen Med J, 2010, 3, 18-27.
      コラーゲン+ヒアルロン酸カプセルを作製し、骨格筋由来細胞のカプセル内培養やマウス前脛骨筋欠損モデルへ移植。
    • Construction and Biofunction of 3-Dimensional Self-assembly Collagen Nanostructure on PLLA Substrate.
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      PLLAフィルムをコラーゲンコーティング。
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      Cell Transplant. 2009;18(5):619-26. PMID: 19775524
      肝細胞や線維芽細胞、ES細胞をコラーゲンビトリゲル上で培養し、凍結保存液へ浸して凍結。
    • Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane.
      Uchino T, Takezawa T, Ikarashi Y.
      Toxicol In Vitro. 2009 Mar;23(2):333-7. PMID: 19121381
      コラーゲンビトリゲルを作製し、線維芽細胞と樹状細胞、角化細胞を播種した皮膚モデルを作製。
    • Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2.
      Zhao J, Shinkai M, Takezawa T, Ohba S, Chung UI, Nagamune T
      J Biosci Bioeng. 2009 Mar;107(3):318-23. PMID: 19269600
      BMP-2含浸コラーゲンビトリゲルを作製し、マウス頭蓋骨欠損部に移植。
    • The osteogenic differentiation of adipose tissue-derived precursor cells in a 3D scaffold/matrix environment.
      Leong DT, Nah WK, Gupta A, Hutmacher DW, Woodruff MA.
      Curr Drug Discov Technol. 2008 Dec;5(4):319-27. PMID: 19075612
      コラーゲン溶液をPCL-TCPスキャフォールドへ添加してスポンジを作製し、脂肪由来幹細胞を培養。
    • Physical and biological properties of collagen-phospholipid polymer hybrid gels.
      Nam K, Kimura T, Kishida A.
      Biomaterials. 2007 Jul;28(20):3153-62. PMID: 17391753
      MPC固定化コラーゲンゲルの作製。
    • Preparation and characterization of cross-linked collagen-phospholipid polymer hybrid gels.
      Nam K, Kimura T, Kishida A.
      Biomaterials. 2007 Jan;28(1):1-8. PMID: 16959313
      MPC固定化コラーゲンゲルの作製。
    • Reconstruction of a hard connective tissue utilizing a pressed silk sheet and type-I collagen as the scaffold for fibroblasts.
      Takezawa T, Ozaki K, Takabayashi C.
      Tissue Eng. 2007 Jun;13(6):1357-66. PMID: 17518702
      圧縮シルクシート含有コラーゲンビトリゲルを作製し、線維芽細胞を培養。圧縮シルクシートのコラーゲンコーティングも。
    • Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo.
      Takezawa T, Takeuchi T, Nitani A, Takayama Y, Kino-Oka M, Taya M, Enosawa S.
      J Biotechnol. 2007 Aug 1;131(1):76-83. PMID: 17624459
      コラーゲンビトリゲルを作製し、褐色細胞腫由来細胞と線維芽細胞を両面培養。
    • Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis.
      Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T.
      Biotechnol Bioeng. 2006 Apr 20;93(6):1152-63. PMID: 16470881
      間葉系幹細胞+コラーゲン+アルギン酸ビーズを作製し、培養。
    • Electrochemical properties of 316L stainless steel with culturing L929 fibroblasts.
      Hiromoto S, Hanawa T.
      J R Soc Interface. 2006 Aug 22;3(9):495-505. PMID: 16849246
      スチールディスクをコラーゲンコーティングし、線維芽細胞を培養。
    • Fabrication of a new tubular fibrous PLCL scaffold for vascular tissue engineering.
      Kim SH, Kwon JH, Chung MS, Chung E, Jung Y, Kim SH, Kim YH.
      J Biomater Sci Polym Ed. 2006;17(12):1359-74. PMID: 17260508
      PLCLファイバーをコラーゲンコーティングし、血管内皮細胞や血管平滑筋を培養。
    • In vitro assays for adhesion and migration of osteoblastic cells (Saos-2) on titanium surfaces.
      Li CY, Gao SY, Terashita T, Shimokawa T, Kawahara H, Matsuda S, Kobayashi N.
      Cell Tissue Res. 2006 Jun;324(3):369-75. PMID: 16450122
      チタン製インプラントをコラーゲンコーティングし、骨芽細胞を培養。
    • Novel tissue-engineered biodegradable material for reconstruction of vascular wall.
      Iwai S, Sawa Y, Taketani S, Torikai K, Hirakawa K, Matsuda H.
      Ann Thorac Surg. 2005 Nov;80(5):1821-7. PMID: 16242461
      コラーゲン+PGAあるいはコラーゲン+PLAスキャフォールドを作製し、血管内皮細胞を培養。
    • Co-electrospun nanofiber fabrics of poly(L-lactide-co-epsilon-caprolactone) with type I collagen or heparin.
      Kwon IK, Matsuda T.
      Biomacromolecules. 2005 Jul-Aug;6(4):2096-105. PMID: 16004450
      コラーゲン+PLCLスキャフォールドを作製し、血管内皮細胞を培養。
    • In vitro and in vivo study of He+ ion irradiated collagen for development of small diameter stent graft material.
      Y. Suzuki, M. Iwaki, N. Takahashi, T. Yotoriyama, K. Kurotobi, H. Ujiie, T. Hori.
      Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Volume 232, Issues 1–4, May 2005, Pages 353-357.
      ステントグラフトをコラーゲンコーティングし、イオンビーム照射。さらにウサギ頸動脈へ移植。
    • The changes of cell adhesion to collagen-coated Al2O3 by ion bombardment.
      Y. Yokoyama, T. Tsukamoto, T. Kobayashi, M. Iwaki.
      Surface and Coatings Technology, Volume 196, Issues 1–3, 22 June 2005, Pages 298-302.
      酸化アルミニウムをコラーゲンコーティングし、大動脈血管内皮細胞を培養。
    • Collagen vitrigel: a novel scaffold that can facilitate a three-dimensional culture for reconstructing organoids.
      Takezawa T, Ozaki K, Nitani A, Takabayashi C, Shimo-Oka T
      Cell Transplant. 2004;13(4):463-73. PMID: 15468688
      コラーゲンビトリゲルを作製し、結腸がん由来細胞と線維芽細胞を両面培養。
    • Adeno-Associated Virus-Encapsulated Alginate Microspheres Loaded in Collagen Gel Carriers for Localized Gene Transfer.
      Kurashina Y, Kurihara S, Kubota T, Takatsuka S, Hirabayashi M, Shimmura H, Miyahara H, Hioki A, Matsushita Y, Muramatsu J, Ogawa Y, Fujioka M, Okano HJ, Onoe H.
      Adv Healthc Mater. 2024 Jan 15:e2303546. doi: 10.1002/adhm.202303546. Epub ahead of print. PMID: 38224572.
      AAV含有アルギン酸マイクロスフェアをコラーゲンゲルで包埋し、マウス筋肉内での徐放に使用。
    • Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks.
      Sato Y, Yamamoto H, Kato H, Tanii T, Sato S, Hirano-Iwata A.
      Front Neurosci. 2023 Jan 9;16:943310. PMID: 36699522.
      電極上にコラーゲンゲルを作製し、ゲル上でラット初代神経細胞を培養。
    • Three-Dimensional Endometrium Embryo-Implantation In Vitro Model Using Cell Sheet Engineering Technology in Rats,x
      Takashi Suzuki, Goro Kuramoto, Katsuhisa Sakaguchi, Tsutomu Tabata, Tatsuya Shimizu,
      Tokyo Women's Medical University Journal, 2023, 7 巻, p. 42-49, https://www.jstage.jst.go.jp/article/twmuj/7/0/7_2022016/_article/-char/ja
      コラーゲン+フィブリンからなるゲル上で、子宮内膜間質細胞シートと子宮内膜上皮細胞シートを培養した上に胚盤胞を配置。
    • In-Vitro Decidualization With Different Progesterone Concentration: Development of a Hormone-Responsive 3D Endometrial Tissue Using Rat Endometrial Tissues.
      Toma C, Kuramoto G, Homma J, Sakaguchi K, Shimizu T.
      Cureus. 2023 Nov 28;15(11):e49613. PMID: 38033443.
      コラーゲン+フィブリンからなるゲル上で、子宮内膜間質細胞シートと子宮内膜上皮細胞シートを培養。
    • Macroscale Collagen-Actomyosin Hybrid Actuator Built from Bioderived Materials.
      K. Yoshida, K. Kohno, Y. Hiratsuka, H. Onoe,
      Adv. Funct. Mater. 2023, 33, 2307766. https://doi.org/10.1002/adfm.202307766
      collagen-actomyosin hybrid soft actuatorを作製。
    • Aberrant TIMP-1 overexpression in tumor-associated fibroblasts drives tumor progression through CD63 in lung adenocarcinoma.
      Duch P, Díaz-Valdivia N, Ikemori R, Gabasa M, Radisky ES, Arshakyan M, Gea-Sorlí S, Mateu-Bosch A, Bragado P, Carrasco JL, Mori H, Ramírez J, Teixidó C, Reguart N, Fillat C, Radisky DC, Alcaraz J.
      Matrix Biol. 2022 Aug;111:207-225. PMID: 35787446.
      コラーゲン溶液とマトリゲルを混合し、肺腺がん細胞をマウス皮下へ移植。
    • The Thermal Stability of the Collagen Triple Helix Is Tuned According to the Environmental Temperature.
      Fujii KK, Taga Y, Takagi YK, Masuda R, Hattori S, Koide T.
      Int J Mol Sci. 2022 Feb 12;23(4):2040. PMID: 35216155.
      コラーゲン溶液を臭化シアン活性化セファロースビーズと混合し、HSP47を濃縮。
    • Overexpression of KiSS1 Induces the Proliferation of Hepatocarcinoma and Increases Metastatic Potential by Increasing Migratory Ability and Angiogenic Capacity.
      Kim CW, Lee HK, Nam MW, Choi Y, Choi KC.
      Mol Cells. 2022 Dec 31;45(12):935-949. PMID: 36572562.
      肝がん細胞スフェロイドをコラーゲンゲル上で培養し、浸潤評価。
    • The role of KiSS1 gene on the growth and migration of prostate cancer and the underlying molecular mechanisms.
      Kim CW, Lee HK, Nam MW, Lee G, Choi KC.
      Life Sci. 2022 Dec 1;310:121009. PMID: 36181862.
      前立腺がん細胞スフェロイドをコラーゲンゲル上で培養し、浸潤評価。
    • Development of a novel method for measuring tissue oxygen pressure to improve the hypoxic condition in subcutaneous islet transplantation.
      Mitsugashira H, Imura T, Inagaki A, Endo Y, Katano T, Saito R, Miyagi S, Watanabe K, Kamei T, Unno M, Goto M.
      Sci Rep. 2022 Aug 30;12(1):14731. PMID: 36042259.
      膵島を含むコラーゲンゲルを酸素送達デバイスと一体化させ、ラット背部へ移植。
    • Perfusable vascular tree like construction in 3D cell-dense tissues using artificial vascular bed.
      Tobe Y, Homma J, Sakaguchi K, Sekine H, Iwasaki K, Shimizu T.
      Microvasc Res. 2022 May;141:104321. PMID: 35032535.
      還流用の流路を持つコラーゲンゲルの上で積層血管内皮細胞シートを培養し、血管床を作成。
    • Mechanical performance of collagen gels is dependent on purity, α1/α2 ratio, and telopeptides.
      Slyker L, Diamantides N, Kim J, Bonassar LJ.
      J Biomed Mater Res A. 2022 Jan;110(1):11-20. PMID: 34236763.
      ウシ皮、ウシ腱、ラット尾のネイティブコラーゲンやアテロコラーゲンの物性比較。
    • Perfusable System Using Porous Collagen Gel Scaffold Actively Provides Fresh Culture Media to a Cultured 3D Tissue.
      Imashiro C, Yamasaki K, Tanaka RI, Tobe Y, Sakaguchi K, Shimizu T.
      Int J Mol Sci. 2021 Jun 24;22(13):6780. PMID: 34202572.
      還流用の流路を持つコラーゲンゲル上で線維芽細胞シートを還流培養。
    • Comparison of the Mechanical Properties Between the Convex and Concave Inner/Apical Surfaces of the Developing Cerebrum.
      Nagasaka A, Miyata T.
      Front Cell Dev Biol. 2021 Jul 23;9:702068. PMID: 34368153.
      コラーゲンゲル上に脳外套を置き、AFMで弾性を評価。
    • Collagen analogs with phosphorylcholine are inflammation-suppressing scaffolds for corneal regeneration from alkali burns in mini-pigs.
      Simpson FC, McTiernan CD, Islam MM, Buznyk O, Lewis PN, Meek KM, Haagdorens M, Audiger C, Lesage S, Gueriot FX, Brunette I, Robert MC, Olsen D, Koivusalo L, Liszka A, Fagerholm P, Gonzalez-Andrades M, Griffith M.
      Commun Biol. 2021 May 21;4(1):608. PMID: 34021240.
      SDS-PAGEの標準として使用。
    • Epigenetic SMAD3 Repression in Tumor-Associated Fibroblasts Impairs Fibrosis and Response to the Antifibrotic Drug Nintedanib in Lung Squamous Cell Carcinoma.
      Ikemori R, Gabasa M, Duch P, Vizoso M, Bragado P, Arshakyan M, Luis IC, Marín A, Morán S, Castro M, Fuster G, Gea-Sorli S, Jauset T, Soucek L, Montuenga LM, Esteller M, Monsó E, Peinado VI, Gascon P, Fillat C, Hilberg F, Reguart N, Alcaraz J.
      Cancer Res. 2020 Jan 15;80(2):276-290. PMID: 31694906
      非小細胞肺癌細胞株をコラーゲンおよびマトリゲルと混合して、マウス背部皮下に移植。
    • Mouse skin fibroblasts with mesenchymal stem cell marker p75 neurotrophin receptor proliferate in response to prolyl-hydroxyproline.
      Tomoko T. Asai, Kazunobu Yoshikawa, Kazuhiro Sawada, Kazuna Fukamizu, Yoh-ichi Koyama, Yasutaka Shigemura, Shiro Jimi, Kenji Sato.
      Journal of Functional Foods, Volume 66, 2020.
      線維芽細胞のコラーゲンゲル上培養。
    • Discovery of Ti-Binding Abilities of Phosphorylated-Chitin and -Collagen.
      Yoshinori Kuboki, Seiichi Tokura, Kimitoshi Yagami, Toshitake Furusawa, Kouichi Morimoto, Qin Song, Souhei (Xiaobing) Iku, Masaaki Kurasaki.
      Journal of Hard Tissue Biology, 2020 29(1):45-48.
      コラーゲンをリン酸化し、チタンとの結合性を評価。
    • Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide.
      Asai TT, Oikawa F, Yoshikawa K, Inoue N, Sato K.
      Int J Mol Sci. 2019 Dec 28;21(1). PMID: 31905705
      線維芽細胞のコラーゲンゲル上培養。
    • Development of a collagen-like peptide polymer via end-to-end disulfide cross-linking and its application as a biomaterial.
      Ichise SF, Takeuchi S, Aoki S, Kuroda KC, Nose H, Masuda R, Koide T
      Acta Biomater. 2019 Aug;94:361-371. PMID: 31200119
    • Analysis of Ultraviolet Radiation Wavelengths Causing Hardening and Reduced Elasticity of Collagen Gels In Vitro.
      Kazuhisa Maeda.
      Cosmetics 2018, 5(1), 14.
      コラーゲンゲルにUVを照射。
    • Three-dimensional printed microfluidic modules for design changeable coaxial microfluidic devices.
      Yuya Morimoto, Mahiro Kiyosawa, Shoji Takeuchi.
      Sensors and Actuators B: Chemical, Volume 274, 2018, Pages 491-500.
    • Amino Carbonylation of Epidermal Basement Membrane Inhibits Epidermal Cell Function and Is Suppressed by Methylparaben.
      Haruka Morimoto , Lihao Gu , Haifeng Zeng and Kazuhisa Maeda.
      Cosmetics 2017, 4(4), 38
      プレートやディッシュ上に糖化コラーゲンゲルを作製し、角化細胞を培養。
    • Vascularized Tissue-Engineered Model for Studying Drug Resistance in Neuroblastoma.
      Villasante A, Sakaguchi K, Kim J, Cheung NK, Nakayama M, Parsa H, Okano T, Shimizu T, Vunjak-Novakovic G.
      Theranostics 2017; 7(17):4099-4117.
      神経芽腫細胞および血管内皮細胞シートを作製し、還流用のポアを持つコラーゲンゲル上で培養。
    • Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty in a rabbit model.
      Hashimoto Y, Hattori S, Sasaki S, Honda T, Kimura T, Funamoto S, Kobayashi H, Kishida A.
      Sci Rep. 2016 Jun 13;6:27734. PMID: 27291975
      コラーゲン溶液に高静水圧をかけ、CDスペクトルを測定。
    • Thermal denaturation behavior of collagen fibrils in wet and dry environment.
      Suwa Y, Nam K, Ozeki K, Kimura T, Kishida A, Masuzawa T.
      J Biomed Mater Res B Appl Biomater. 2016 Apr;104(3):538-45 PMID: 25952296
      コラーゲン線維の熱変性を評価。
    • Synergistic rate boosting of collagen fibrillogenesis in heterogeneous mixtures of crowding agents.
      Dewavrin JY, Abdurrahiem M, Blocki A, Musib M, Piazza F, Raghunath M.
      J Phys Chem B. 2015 Mar 26;119(12):4350-8. PMID: 25730613.
      分子クラウディングを応用したコラーゲンの線維形成チューニング。
    • Cellular characterization of thrombocytes in Xenopus laevis with specific monoclonal antibodies.
      Tanizaki Y, Ishida-Iwata T, Obuchi-Shimoji M, Kato T.
      Exp Hematol. 2015 Feb;43(2):125-36. PMID: 25448492
      血小板凝集の評価。
    • Tuning the architecture of three-dimensional collagen hydrogels by physiological macromolecular crowding.
      Dewavrin JY, Hamzavi N, Shim VP, Raghunath M.
      Acta Biomater. 2014 Oct;10(10):4351-9. PMID: 24932771
      分子クラウディングを応用したコラーゲンの線維形成チューニングを行い、間葉系幹細胞をゲル上培養。
    • Migration of breast cancer cells into reconstituted type I collagen gels assessed via a combination of frozen sectioning and azan staining.
      Fukuda K, Kamoshida Y, Kurokawa T, Yoshida M, Fujita-Yamaguchi Y, Nakata M.
      Biosci Trends. 2014 Aug;8(4):212-6. PMID: 25224627
      乳がん細胞をコラーゲンゲル上培養し、浸潤評価。
    • Overexpression of integrin αv facilitates proliferation and invasion of oral squamous cell carcinoma cells via MEK/ERK signaling pathway that is activated by interaction of integrin αvβ8 with type Ⅰ collagen.
      Hayashido Y, Kitano H, Sakaue T, Fujii T, Suematsu M, Sakurai S, Okamoto T.
      Int J Oncol. 2014 Nov;45(5):1875-82. PMID: 25190218
      口腔扁平上皮がん細胞をゲル上培養し、浸潤評価。
    • Relationships between molecular mobility, fibrillogenesis of collagen molecules, and the inflammatory response: An experimental study in vitro and in vivo.
      Nam K, Seo JH, Kimura T, Yui N, Kishida A.
      J Colloid Interface Sci. 2014 Jul 1;433C:16-25. PMID: 25112908
      ABA型ブロック共重合体上でコラーゲンの線維化を評価。
    • In vitro engineering of vascularized tissue surrogates.
      Sakaguchi K, Shimizu T, Horaguchi S, Sekine H, Yamato M, Umezu M, Okano T.
      Sci Rep. 2013 Feb 19;3:1316. PMID: 23419835
      心筋細胞および血管内皮細胞からなる心筋シートを作製し、還流用のポアを持つコラーゲンゲル上で培養。
    • High-throughput turbidimetric screening for heparin-neutralizing agents and low-molecular-weight heparin mimetics.
      Sekiya A, Oishi S, Fujii N, Koide T.
      Chem Pharm Bull (Tokyo). 2012;60(3):371-6. PMID:22382418
      コラーゲンの線維形成を評価。
    • Rapid formation of size-controlled three dimensional hetero-cell aggregates using micro-rotation flow for spheroid study.
      Ota H, Kodama T, Miki N.
      Biomicrofluidics. 2011 Sep;5(3):34105-3410515. PMID: 22662035
      肝がん細胞表面をコラーゲンコーティングし、血管内皮細胞とヘテロ凝集塊を形成。
    • Invasion of carcinoma cells into reconstituted type I collagen gels: visual real-time analysis by time-lapse microscopy.
      Sakai K, Kurokawa T, Furui Y, Kuronuma Y, Sekiguchi M, Ando J, Inagaki Y, Tang W, Nakata M, Fujita-Yamaguchi Y.
      Biosci Trends. 2011;5(1):10-6. PMID: 21422595
      乳がん細胞をコラーゲンゲル上培養し、浸潤のタイムラプスイメージング。
    • TGF-β regulates isoform switching of FGF receptors and epithelial-mesenchymal transition.
      Shirakihara T, Horiguchi K, Miyazawa K, Ehata S, Shibata T, Morita I, Miyazono K, Saitoh M.
      EMBO J. 2011 Feb 16;30(4):783-95. PMID: 21224849
      ヒト乳腺がん細胞をコラーゲンゲル上培養し、浸潤評価。
    • Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions.
      Lareu RR, Zeugolis DI, Abu-Rub M, Pandit A, Raghunath M.
      Acta Biomater. 2010 Aug;6(8):3146-51. PMID: 20144751
      コラーゲン定量。
    • Immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells.
      Kubo C, Tsutsui TW, Tamura Y, Kumakura S, Tsutsui T.
      Odontology. 2009 Jan;97(1):18-31. PMID: 19184294
      歯肉角化細胞のコラーゲンゲル上培養。
    • Effect of Prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin.
      Shigemura Y, Iwai K, Morimatsu F, Iwamoto T, Mori T, Oda C, Taira T, Park EY, Nakamura Y, Sato K.
      J Agric Food Chem. 2009 Jan 28;57(2):444-9. PMID: 19128041
      線維芽細胞のコラーゲンゲル上培養。
    • Self-assembly nano-structure of type I collagen adsorbed on Gemini surfactant LB monolayers.
      Xu S, Liu A, Chen Q, Lv M, Yonese M, Liu H.
      Colloids Surf B Biointerfaces. 2009 Apr 1;70(1):124-31. PMID: 19157808
      コラーゲンをアニオン性ジェミニ型界面活性剤Langmuir-Blodgett膜へ吸着。
    • Auto-organized nano-structure of collagen on Gemini surfactant monolayer.
      Lv M, Chen Q, Yonese M, Xu S, Liu H.
      Colloids Surf B Biointerfaces. 2008 Feb 15;61(2):282-9. PMID: 17933500
      コラーゲンをジェミニ型界面活性剤膜へ吸着。
    • Controlling coupling reaction of EDC and NHS for preparation of collagen gels using ethanol/water co-solvents.
      Nam K, Kimura T, Kishida A.
      Macromol Biosci. 2008 Jan 9;8(1):32-7. PMID: 18023082
      コラーゲンゲルの架橋を制御。
    • Network structure of collagen layers absorbed on LB film.
      Chen Q, Xu S, Li R, Liang X, Liu H.
      J Colloid Interface Sci. 2007 Dec 1;316(1):1-9. PMID: 17727873
      コラーゲンをLangmuir-Blodgett膜へ吸着。
    • Transplantation of engineered bone tissue using a rotary three-dimensional culture system.
      Hidaka M, Su GN, Chen JK, Mukaisho K, Hattori T, Yamamoto G.
      In Vitro Cell Dev Biol Anim. 2007 Feb;43(2):49-58. PMID: 17570019
      頭蓋骨由来細胞含有コラーゲンゲルをHARVバイオリアクター中で培養し、マウス背部やラット頭蓋骨欠損部へ移植。
    • Differentiation of tracheal basal cells to ciliated cells and tissue reconstruction on the synthesized basement membrane substratum in vitro.
      Hosokawa T, Betsuyaku T, Nishimura M, Furuyama A, Katagiri K, Mochitate K.
      Connect Tissue Res. 2007;48(1):9-18. PMID: 17364662
      気官上皮細胞のコラーゲンゲル上培養。プレートのコラーゲンコーティングも。
    • Intracellular events in retinal glial cells exposed to ICG and BBG.
      Kawahara S, Hata Y, Miura M, Kita T, Sengoku A, Nakao S, Mochizuki Y, Enaida H, Ueno A, Hafezi-Moghadam A, Ishibashi T.
      Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4426-32. PMID: 17898261
      網膜グリア細胞のコラーゲンゲル上培養。
    • Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: the biological relevance of the excluded volume effect.
      Lareu RR, Subramhanya KH, Peng Y, Benny P, Chen C, Wang Z, Rajagopalan R, Raghunath M.
      FEBS Lett. 2007 Jun 12;581(14):2709-14. PMID: 17531987
      SDS-PAGEのコントロール。
    • Targeted mutation of serine 697 in the Ret tyrosine kinase causes migration defect of enteric neural crest cells.
      Asai N, Fukuda T, Wu Z, Enomoto A, Pachnis V, Takahashi M, Costantini F.
      Development. 2006 Nov;133(22):4507-16. PMID: 17050626
      マウス中腸部のコラーゲンゲル上培養。
    • The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes.
      Hoshiba T, Cho CS, Murakawa A, Okahata Y, Akaike T.
      Biomaterials. 2006 Sep;27(26):4519-28. PMID: 16697038
      PVLAあるいはPLLコーティングディッシュ/プレートへのコラーゲン付着を評価。
    • Initial bFGF Distribution Affects the Depth of Three-dimensional Microvessel Networks in Vitro.
      Akinori UEDA, Ryo SUDO, Mariko IKEDA, Susumu KUDO and Kazuo TANISHITA.
      Journal of Biomechanical Science and Engineering, Vol. 1, No. 1 (2006), pp.136-146.
      血管内皮細胞のコラーゲンゲル上培養。
    • Hepatocyte growth factor inhibits the formation of the basement membrane of alveolar epithelial cells in vitro.
      Furuyama A1, Mochitate K.
      Am J Physiol Lung Cell Mol Physiol. 2004 May;286(5):L939-46. PMID: 14672920
      セルカルチャーインサート上にコラーゲンゲルを作製し、肺胞上皮細胞を培養。
    • The combination of SOX5, SOX6, and SOX9 (the SOX trio) provides signals sufficient for induction of permanent cartilage.
      Ikeda T, Kamekura S, Mabuchi A, Kou I, Seki S, Takato T, Nakamura K, Kawaguchi H, Ikegawa S, Chung UI.
      Arthritis Rheum. 2004 Nov;50(11):3561-73. PMID: 15529345
      間葉系幹細胞や線維芽細胞のコラーゲンゲル上培養。
    • Inhibitory effects of 9-(4-thio-beta-D-ribo-pentofuranosyl)guanine on tumor growth and angiogenesis.
      Miura S, Yamada K, Kano F, Yoshimura Y.
      Biol Pharm Bull. 2004 Apr;27(4):520-3. PMID: 15056858
      肉腫皮下腫瘍モデルより摘出した腫瘍片をコラーゲンゲル上培養。
    • Stimulation of in vitro angiogenesis by nitric oxide through the induction of transcription factor ETS-1.
      Shimizu S, Kageyama M, Yasuda M, Sasaki D, Naito S, Yamamoto T, Kiuchi Y.
      Int J Biochem Cell Biol. 2004 Jan;36(1):114-22. PMID: 14592537
      血管内皮細胞のコラーゲンゲル上培養。
    • Immunohistochemical Identification of HSP47 and Type I Procollagen-expressing Cells in Mongolian Gerbil Salivary Glands: Correlation with Age-related Fibrotic Changes.
      Harumi Tomisaki, Joji Sekine, Tsugio Inokuchi, Shin-ichi Izumi, Kunio Takano.
      Oral Medicine & Pathology. 9(4), 141-148, 2004.
      ウェスタンブロッティングのコントロール。
    • Effect on endothelial cell gene expression of shear stress, oxygen concentration, and low-density lipoprotein as studied by a novel flow cell culture system.
      Warabi E, Wada Y, Kajiwara H, Kobayashi M, Koshiba N, Hisada T, Shibata M, Ando J, Tsuchiya M, Kodama T, Noguchi N.
      Free Radic Biol Med. 2004 Sep 1;37(5):682-94. PMID: 15288125
      セルカルチャーインサート上にコラーゲンゲルを形成し、血管内皮細胞を培養。
    • The involvement of integrin alphavbeta3 in polymorphonuclear leukocyte-induced angiogenesis in bovine aortic endothelial cells.
      Yasuda M, Ohbayashi M, Ohhinata K, Yamamoto T.
      Life Sci. 2004 Jun 11;75(4):421-34. PMID: 15147829
      血管内皮細胞をコラーゲンゲル上培養。
    • Intralumenal tissue-engineered therapeutic stent using endothelial progenitor cell-inoculated hybrid tissue and in vitro performance.
      Shirota T, Yasui H, Matsuda T.
      Tissue Eng. 2003 Jun;9(3):473-85. PMID: 12857415
      血管内皮前駆細胞含有コラーゲンゲルをステントの周囲に形成。その周りに血管平滑筋含有コラーゲンゲルを作製。
    • Newly designed compliant hierarchic hybrid vascular graft wrapped with microprocessed elastomeric film--II: Morphogenesis and compliance change upon implantation.
      He H, Matsuda T.
      Cell Transplant. 2002;11(1):75-87. PMID: 12095223
      血管平滑筋を含むゲルを棒の周囲に形成し、ウレタンスポンジを巻きつけた後、その周囲にイヌ血管内皮細胞を含むゲルを作製。
    • Effect of dexamethasone on invasion of human squamous cell carcinoma cells into collagen gel.
      Hayashido Y, Shirasuna K, Sugiura T, Nakashima M, Matsuya T.
      Cancer Lett. 1996 Nov 12;108(1):81-6. PMID: 8950213
      口腔扁平上皮がん細胞をコラーゲンゲル上培養し、浸潤評価。
    • Serum-free medium conditions for steroidogenesis of bovine follicular thecal cells cultured on collagen gel matrix.
      Ikeda H.
      In Vitro Cell Dev Biol. 1990 Feb;26(2):193-200. PMID: 2312503
      卵巣莢膜細胞をコラーゲンゲル上培養。