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AteloGene® Local Use “Quick Gelation”(徐放性局所投与用)

概要

AteloGene® Local Useのゲル化速度と核酸導入効果を向上させた改良品!

本製品はマウスへの局所投与により siRNA/miRNA を生体組織中へ導入し、効率よく細胞内へ導入するためのin vivoトランスフェクション試薬です。
「AteloGene®」の主成分であるアテロコラーゲンは、正に荷電しているため、適切な濃度と割合で核酸と混合することにより、 静電的に複合体を形成します。そして、複合体形成により、in vivoでの核酸分解酵素による核酸の分解を防ぐことが可能です 。

アテロコラーゲンとは

用途

核酸の局所投与

特長

  • 「AteloGene®」の主成分であるアテロコラーゲンは、siRNA/miRNAと複合体を形成するため、in vivoでのRNAi効果が長続きします。
  • 「AteloGene®」はアテロコラーゲンから作られているため、無毒性です。
  • 「AteloGene® Local Use」は生体内でゲル化する性質を持っているため、siRNA/miRNAが投与部位に留まり、in vivoでの核酸導入効果を高める徐放効果が得られます。
  • 「AteloGene® Local Use “Quick Gelation”」は投与部位でのゲル形成速度を高めることで、従来品よりもin vivoでの核酸導入効果が向上しています。
 

キットの内容

①AteloGene® Local Use “Quick Gelation”
プレフィルドシリンジ
(調製中のロスも含めて540μLで5回投与分)
540 μL入り × 3 本
②QGバッファー 1.5 mL × 3本
③2mLマイクロチューブ 4本 (予備のチューブ1本を含む)
④18G注射針 8本 (予備の針2本を含む)

*QGバッファーの容量、包装が変更になりました。キットには1回使い切りタイプ1.5mLが3本含まれております。

キット以外に必要な機器・試薬

  • 1mLディスポーザブルシリンジ
  • 27G注射針
  • siRNA/miRNA(AteloSiLence in vivo 用siRNA/miRNAのようなPAGE精製またはHPLC精製グレード)
  • siRNA/miRNA調製用容器および水(滅菌済み、RNase free)
  • 保冷具(クラッシュアイス、コールドブロックなど)
  • ローテーター(転倒攪拌できるもの、TAITEC RT-5、Bibby scientific SB3など)
  • ピペッターおよびチップ(滅菌済み、RNase free)
  • 高速冷却遠心機
  • 麻酔薬
製品番号 製品 包装 価格
#1490 AteloGene® Local Use “Quick Gelation” 1キット(15回投与分) ¥75,000
(税込¥82,500)

※200μL/回として算出しているため、対象組織によっては15回以上使用可能な場合もあります。

保管方法:冷蔵(2〜10℃) 本品は研究用試薬です。
代理店への仕切り価格は、家田化学薬品株式会社(03-3816-1871)へご確認ください。

使用例

【AteloGene® Local Use “Quick Gelation”を用いたマウス皮下腫瘍モデルへのsiRNA局所投与】

マウス皮下に移植したデュアルルシフェラーゼ発現メラノーマ細胞に対し、「AteloGene® Local Use “QuickGelation”(QG)」を使用してルシフェラーゼsiRNA(Luc siRNA)を局所投与すると、顕著なルシフェラーゼ発現抑制効果が認められた。

参考文献

  • がん関連
  • 運動器
  • 皮膚
  • 循環器
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  • その他
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    • Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.
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    • Ephrin-B2 reverse signaling regulates progression and lymph node metastasis of oral squamous cell carcinoma.
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    • Radiation-inducible miR-770-5p sensitizes tumors to radiation through direct targeting of PDZ-binding kinase.
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      HCT116細胞(大腸がん細胞)の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • The microRNA miR-516a-3p regulates the Wnt pathway by targeting extracellular sulfatase 1
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    • ANKRD22 promotes progression of non-small cell lung cancer through transcriptional up-regulation of E2F1.
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      H1299細胞(肺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Oncogenic role of rab escort protein 1 through EGFR and STAT3 pathway.
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      A431細胞(類表皮がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Pharmacological regulation of bladder cancer by miR-130 family seed-targeting LNA.
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      5637細胞(膀胱がん細胞)の皮下腫瘍モデルマウスへのLNA局所投与。
    • Nek2 siRNA therapy using a portal venous port-catheter system for liver metastasis in pancreatic cancer.
      Kokuryo T, Hibino S, Suzuki K, Watanabe K, Yokoyama Y, Nagino M, Senga T, Hamaguchi M.
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      KLM1細胞(膵臓がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • A therapeutic trial of human melanomas with combined small interfering RNAs targeting adaptor molecules p130Cas and paxillin activated under expression of ganglioside GD3.
      Makino Y, Hamamura K, Takei Y, Bhuiyan RH, Ohkawa Y, Ohmi Y, Nakashima H, Furukawa K, Furukawa K.
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      メラノーマ細胞の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Docetaxel induced-JNK2/PHD1 signaling pathway increases degradation of HIF-1α and causes cancer cell death under hypoxia.
      Oh ET, Kim CW, Kim SJ, Lee JS, Hong SS, Park HJ.
      Sci Rep. 2016 Jun 6;6:27382. PMID: 27263528
      MDA-MB-231細胞(乳がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Unfavorable neuroblastoma prognostic factor NLRR2 inhibits cell differentiation by transcriptional induction through JNK pathway.
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      Cancer Sci. 2016 Sep;107(9):1223-32. PMID: 27357360
      SK‐N‐BE細胞(神経芽細胞腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Regulation of SLD5 gene expression by miR-370 during acute growth of cancer cells.
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    • Usefulness of microRNA‑375 as a prognostic and therapeutic tool in esophageal squamous cell carcinoma.
      Isozaki, Y., Hoshino, I., Akutsu, Y., Hanari, N., Mori, M., Nishimori, T., Murakami, K., Akanuma, N., Takeshita, N., Maruyama, T., Toyozumi, T., Takahashi, M., Suito, H., & Matsubara, H.
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      T.Tn細胞およびTE-2細胞(食道扁平上皮がん細胞)の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Arl4c expression in colorectal and lung cancers promotes tumorigenesis and may represent a novel therapeutic target.
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      Oncogene. 2015 Sep 10;34(37):4834-44. PMID: 25486429
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    • MiR-634 activates the mitochondrial apoptosis pathway and enhances chemotherapy-induced cytotoxicity.
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      Cancer Res. 2015 Sep 15;75(18):3890-901. PMID: 26216549
      KYSE170細胞(食道扁平上皮がん細胞)の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • RFPL4A increases the G1 population and decreases sensitivity to chemotherapy in human colorectal cancer cells.
      Naito A, Yamamoto H, Kagawa Y, Naito Y, Okuzaki D, Otani K, Iwamoto Y, Maeda S, Kikuta J, Nishikawa K, Uemura M, Nishimura J, Hata T, Takemasa I, Mizushima T, Ishii H, Doki Y, Mori M, Ishii M.
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    • Efficient inhibition of tumor angiogenesis and growth by a synthetic peptide blocking S100A4-methionine aminopeptidase 2 interaction.
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      PC-3M-Luc細胞(前立腺がん細胞)の皮下腫瘍モデルマウスへのペプチド局所投与。
    • Ubiquilin 2 enhances osteosarcoma progression through resistance to hypoxic stress.
      Tsukamoto S, Shimada K, Honoki K, Kido A, Akahane M, Tanaka Y, Konishi N.
      Oncol Rep. 2015 Apr;33(4):1799-806. PMID: 25672654
      COS1NR細胞(骨肉腫細胞)の皮下腫瘍モデルラットへのsiRNA局所投与。
    • APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer.
      Periyasamy M, Patel H, Lai CF, Nguyen VT, Nevedomskaya E, Harrod A, Russell R, Remenyi J, Ochocka AM, Thomas RS, Fuller-Pace F, Győrffy B, Caldas C, Navaratnam N, Carroll JS, Zwart W, Coombes RC, Magnani L, Buluwela L, Ali S.
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      MCF-7細胞(乳がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Aurora kinase A has a significant role as a therapeutic target and clinical biomarker in endometrial cancer.
      Umene K, Yanokura M, Banno K, Irie H, Adachi M, Iida M, Nakamura K, Nogami Y, Masuda K, Kobayashi Y, Tominaga E, Aoki D.
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      HEC-1B細胞(子宮体がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Glyoxalase I is differentially expressed in cutaneous neoplasms and contributes to the progression of squamous cell carcinoma.
      Zou XY, Ding D, Zhan N, Liu XM, Pan C, Xia YM.
      J Invest Dermatol. 2015 Feb;135(2):589-98. PMID: 25184957
      SCC-13細胞(扁平上皮がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • RNA interference against cancer/testis genes identifies DUSP21 as a potential therapeutic target in human hepatocellular carcinoma.
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      Hepatology. 2014 Feb;59(2):518-30. PMID: 23929653
      MHCC‐97H(肝細胞がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • MicroRNAs cooperatively inhibit a network of tumor suppressor genes to promote pancreatic tumor growth and progression.
      Frampton AE, Castellano L, Colombo T, Giovannetti E, Krell J, Jacob J, Pellegrino L, Roca-Alonso L, Funel N, Gall TM, De Giorgio A, Pinho FG, Fulci V, Britton DJ, Ahmad R, Habib NA, Coombes RC, Harding V, Knösel T, Stebbing J, Jiao LR.
      Gastroenterology. 2014 Jan;146(1):268-77.e18. PMID: 24120476
      MIAPaca-2細胞およびPANC-1細胞(膵臓がん細胞)の皮下腫瘍モデルマウスへのmiRNA inhibitor局所投与。
    • Guanine nucleotide-binding protein 1 is one of the key molecules contributing to cancer cell radioresistance.
      Fukumoto M, Amanuma T, Kuwahara Y, Shimura T, Suzuki M, Mori S, Kumamoto H, Saito Y, Ohkubo Y, Duan Z, Sano K, Oguchi T, Kainuma K, Usami S, Kinoshita K, Lee I, Fukumoto M.
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      SAS細胞およびSAS-R1細胞(口腔扁平上皮がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Paired box 2 upregulates androgen receptor gene expression in androgen-independent prostate cancer.
      Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Hongo F, Kamoi K, Okihara K, Kawauchi A, Miki T.
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      22Rv1細胞(前立腺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • High expression of protein tyrosine kinase 7 significantly associates with invasiveness and poor prognosis in intrahepatic cholangiocarcinoma.
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      HuCCT細胞(胆管がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma.
      Migita K, Sho M, Shimada K, Yasuda S, Yamato I, Takayama T, Matsumoto S, Wakatsuki K, Hotta K, Tanaka T, Ito M, Konishi N, Nakajima Y.
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      Colon-26細胞(大腸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Targeting CB2-GPR55 Receptor Heteromers Modulates Cancer Cell Signaling.
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      T98G(神経膠芽腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-296/Scribble axis is deregulated in human breast cancer and miR-296 restoration reduces tumour growth in vivo.
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      MDA-MB-231細胞(乳がん細胞)の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • AFAP1L1, a novel associating partner with vinculin, modulates cellular morphology and motility, and promotes the progression of colorectal cancers.
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      RKO細胞、LoVo細胞、SW480細胞(大腸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • BMP4/Thrombospondin-1 loop paracrinically inhibits tumor angiogenesis and suppresses the growth of solid tumors.
      Tsuchida R, Osawa T, Wang F, Nishii R, Das B, Tsuchida S, Muramatsu M, Takahashi T, Inoue T, Wada Y, Minami T, Yuasa Y, Shibuya M.
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      HeLa細胞(子宮頸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Potential of tumor-suppressive miR-596 targeting LGALS3BP as a therapeutic agent in oral cancer.
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    • Y-box binding protein-1 regulates cell proliferation and is associated with clinical outcomes of osteosarcoma.
      Fujiwara-Okada Y, Matsumoto Y, Fukushi J, Setsu N, Matsuura S, Kamura S, Fujiwara T, Iida K, Hatano M, Nabeshima A, Yamada H, Ono M, Oda Y, Iwamoto Y.
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    • miR-146a enhances the oncogenicity of oral carcinoma by concomitant targeting of the IRAK1, TRAF6 and NUMB genes.
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    • Ubiquitin C-terminal Hydrolase L1 (UCH-L1) Acts as a Novel Potentiator of Cyclin-dependent Kinases to Enhance Cell Proliferation, Independent of its Hydrolase Activity.
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    • Cell cycle-dependent Rho GTPase activity dynamically regulates cancer cell motility and invasion in vivo.
      Kagawa Y, Matsumoto S, Kamioka Y, Mimori K, Naito Y, Ishii T, Okuzaki D, Nishida N, Maeda S, Naito A, Kikuta J, Nishikawa K, Nishimura J, Haraguchi N, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Ishii H, Doki Y, Matsuda M, Kikuchi A, Mori M, Ishii M.
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    • In vivo delivery of siRNA targeting vasohibin-2 decreases tumor angiogenesis and suppresses tumor growth in ovarian cancer.
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      DISS細胞およびSKOV‐3細胞(卵巣がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Targeting the Vav3 oncogene enhances docetaxel-induced apoptosis through the inhibition of androgen receptor phosphorylation in LNCaP prostate cancer cells under chronic hypoxia.
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      LNCaPH細胞(前立腺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • MicroRNA-196a is a putative diagnostic biomarker and therapeutic target for laryngeal cancer.
      Saito K, Inagaki K, Kamimoto T, Ito Y, Sugita T, Nakajo S, Hirasawa A, Iwamaru A, Ishikura T, Hanaoka H, Okubo K, Onozaki T, Zama T.
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      JHU-011細胞(喉頭がん細胞)の同所移植モデルマウスへのmiRNA inhibitor局所投与。
    • YB-1 suppression induces STAT3 proteolysis and sensitizes renal cancer to interferon-α.
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      腎細胞がん細胞の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Temozolomide suppresses MYC via activation of TAp63 to inhibit progression of human glioblastoma.
      Yamaki T, Suenaga Y, Iuchi T, Alagu J, Takatori A, Itami M, Araki A, Ohira M, Inoue M, Kageyama H, Yokoi S, Saeki N, Nakagawara A.
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      U87MG細胞(神経膠芽腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Plasma membrane-associated sialidase (NEU3) regulates progression of prostate cancer to androgen-independent growth through modulation of androgen receptor signaling.
      Kawamura S, Sato I, Wada T, Yamaguchi K, Li Y, Li D, Zhao X, Ueno S, Aoki H, Tochigi T, Kuwahara M, Kitamura T, Takahashi K, Moriya S, Miyagi T.
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      PC-3細胞(前立腺がん細胞の方)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Wig1 prevents cellular senescence by regulating p21 mRNA decay through control of RISC recruitment.
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      H460細胞(肺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Anti-tumor effect of AlkB homolog 3 knockdown in hormone-independent prostate cancer cells.
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      DU145細胞(前立腺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Up-regulation of endogenous PML induced by a combination of interferon-beta and temozolomide enhances p73/YAP-mediated apoptosis in glioblastoma.
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    • D-amino acid oxidase gene therapy sensitizes glioma cells to the antiglycolytic effect of 3-bromopyruvate.
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    • ALKBH3 contributes to survival and angiogenesis of human urothelial carcinoma cells through NADPH oxidase and Tweak/Fn14/VEGF signals.
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    • ZDHHC8 knockdown enhances radiosensitivity and suppresses tumor growth in a mesothelioma mouse model.
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    • Therapeutic Potential of SOX2 Inhibition for Embryonal Carcinoma.
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    • NKX2-1/TITF1/TTF-1-Induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma.
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    • PCA-1/ALKBH3 contributes to pancreatic cancer by supporting apoptotic resistance and angiogenesis.
      Yamato I, Sho M, Shimada K, Hotta K, Ueda Y, Yasuda S, Shigi N, Konishi N, Tsujikawa K, Nakajima Y.
      Cancer Res. 2012 Sep 15;72(18):4829-39. PMID:22826605
      PANC-1細胞(膵臓がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。また、同所移植モデルへのsiRNA局所投与。
    • The orphan G protein-coupled receptor GPR55 promotes cancer cell proliferation via ERK.
      Andradas C, Caffarel MM, Pérez-Gómez E, Salazar M, Lorente M, Velasco G, Guzmán M, Sánchez C.
      Oncogene. 2011 Jan 13;30(2):245-52. PMID:20818416
      T98G細胞(神経膠芽腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • RECQL1 and WRN proteins are potential therapeutic targets in head and neck squamous cell carcinoma.
      Arai A, Chano T, Futami K, Furuichi Y, Ikebuchi K, Inui T, Tameno H, Ochi Y, Shimada T, Hisa Y, Okabe H.
      Cancer Res. 2011 Jul 1;71(13):4598-607. PMID:21571861
      FaDu細胞(咽頭がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • GEP100-Arf6-AMAP1-cortactin pathway frequently used in cancer invasion is activated by VEGFR2 to promote angiogenesis.
      Hashimoto A, Hashimoto S, Ando R, Noda K, Ogawa E, Kotani H, Hirose M, Menju T, Morishige M, Manabe T, Toda Y, Ishida S, Sabe H.
      PLoS One. 2011;6(8):e23359. PMID:21858086
      MDA-MB-231細胞(乳がん細胞)が播種されたangioreacter tube内へのsiRNA局所投与。
    • Transient silencing of galectin-3 expression promotes both in vitro and in vivo drug-induced apoptosis of human pancreatic carcinoma cells.
      Kobayashi T, Shimura T, Yajima T, Kubo N, Araki K, Wada W, Tsutsumi S, Suzuki H, Kuwano H, Raz A.
      Clin Exp Metastasis. 2011 Apr;28(4):367-76. PMID:21331750
      MIA-PaCa2細胞(膵臓がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Stimulation of the midkine/ALK axis renders glioma cells resistant to cannabinoid antitumoral action.
      Lorente M, Torres S, Salazar M, Carracedo A, Hernández-Tiedra S, Rodríguez-Fornés F, García-Taboada E, Meléndez B, Mollejo M, Campos-Martín Y, Lakatosh SA, Barcia J, Guzmán M, Velasco G.
      Cell Death Cell Death Differ. 2011 Jun;18(6):959-73. PMID:21233844
      U-87MG細胞およびT98G細胞(神経膠芽腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-135b mediates NPM-ALK-driven oncogenicity and renders IL-17-producing immunophenotype to anaplastic large cell lymphoma.
      Matsuyama H, Suzuki HI, Nishimori H, Noguchi M, Yao T, Komatsu N, Mano H, Sugimoto K, Miyazono K.
      Blood. 2011 Dec 22;118(26):6881-92. PMID:22042699
      KARPAS 299細胞(未分化大細胞リンパ腫細胞)の皮下腫瘍モデルマウスへのLNA局所投与。
    • ZNF689 suppresses apoptosis of hepatocellular carcinoma cells through the down-regulation of Bcl-2 family members.
      Shigematsu S, Fukuda S, Nakayama H, Inoue H, Hiasa Y, Onji M, Higashiyama S.
      Exp Cell Res. 2011 Aug 1;317(13):1851-9. PMID:21624362
      HepG2細胞(肝細胞がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • The metastasis-associated microRNA miR-516a-3p is a novel therapeutic target for inhibiting peritoneal dissemination of human scirrhous gastric cancer.
      Takei Y, Takigahira M, Mihara K, Tarumi Y, Yanagihara K.
      Cancer Res. 2011 Feb 15;71(4):1442-1453. PMID:21169410
      44As3細胞(スキルス胃がん細胞)の同所移植モデルマウスへのmiRNA発現べクター局所投与。
    • Synthetic siRNA targeting the breakpoint of EWS/Fli-1 inhibits growth of Ewing sarcoma xenografts in a mouse model.
      Takigami I, Ohno T, Kitade Y, Hara A, Nagano A, Kawai G, Saitou M, Matsuhashi A, Yamada K, Shimizu K.
      Int J Cancer. 2011 Jan 1;128(1):216-26. PMID:20648560
      TC‐135細胞(ユーイング肉腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • ALKBH3, a human AlkB homologue, contributes to cell survival in human non-small-cell lung cancer.
      Tasaki M, Shimada K, Kimura H, Tsujikawa K, Konishi N.
      Br J Cancer. 2011 Feb 15;104(4):700-6. PMID:21285982
      A549細胞およびRERF-LC-AI細胞(肺がん細胞)の腹膜播種モデルマウスへのsiRNA腹腔内投与。
    • Suppression of SOCS3 increases susceptibility of renal cell carcinoma to interferon-α.
      Tomita S, Ishibashi K, Hashimoto K, Sugino T, Yanagida T, Kushida N, Shishido K, Aikawa K, Sato Y, Suzutani T, Yamaguchi O.
      Cancer Sci. 2011 Jan;102(1):57-63. PMID:21054677
      786‐O細胞(腎細胞がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-152 is a tumor suppressor microRNA that is silenced by DNA hypermethylation in endometrial cancer.
      Tsuruta T, Kozaki K, Uesugi A, Furuta M, Hirasawa A, Imoto I, Susumu N, Aoki D, Inazawa J.
      Cancer Res. 2011 Oct 15;71(20):6450-62. PMID:21868754
      HEC-1細胞(子宮体がん細胞)の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Anti-tumoral action of cannabinoids on hepatocellular carcinoma: role of AMPK-dependent activation of autophagy.
      Vara D, Salazar M, Olea-Herrero N, Guzmán M, Velasco G, Díaz-Laviada I.
      Cell Death Differ. 2011 Jul;18(7):1099-111. PMID:21475304
      HepG2細胞(肝細胞がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • The cancer/testis antigen prostate-associated gene 4 (PAGE4) is a highly intrinsically disordered protein.
      Zeng Y, He Y, Yang F, Mooney S, Getzenberg R, Orban J, Kulkarni P. 
      J Biol Chem. 2011 Apr 22;286(16):13985-94. PMID:21357425
      LNCaP細胞(前立腺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Atelocollagen-delivered siRNA targeting the FABP5 gene as an experimental therapy for prostate cancer in mouse xenografts.
      Forootan SS, Bao ZZ, Forootan FS, Kamalian L, Zhang Y, Bee A, Foster CS, Ke Y.
      Int J Oncol. 2010 Jan;36(1):69-76. PMID:19956834
      PC3-M細胞(前立腺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Shank-interacting protein-like 1 promotes tumorigenesis via PTEN inhibition in human tumor cells.
      He L, Ingram A, Rybak AP, Tang D.
      J Clin Invest. 2010 Jun 1;120(6):2094-108. PMID:20458142
      HeLa細胞(子宮頸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-31 ablates expression of the HIF regulatory factor FIH to activate the HIF pathway in head and neck carcinoma.
      Liu CJ, Tsai MM, Hung PS, Kao SY, Liu TY, Wu KJ, Chiou SH, Lin SC, Chang KW.
      Cancer Res. 2010 Feb 15;70(4):1635-44. PMID:20145132
      SAS細胞(口腔扁平上皮がん細胞)の皮下腫瘍モデルマウスへのLNA局所投与。
    • Cyclin K and cyclin D1b are oncogenic in myeloma cells.
      Marsaud V, Tchakarska G, Andrieux G, Liu JM, Dembele D, Jost B, Wdzieczak-Bakala J, Renoir JM, Sola B.
      Mol Cancer. 2010 May 10;9:103. PMID:20459741
      LP-1細胞(骨髄腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • EWS/Fli-1 chimeric fusion gene upregulates vascular endothelial growth factor-A.
      Nagano A, Ohno T, Shimizu K, Hara A, Yamamoto T, Kawai G, Saitou M, Takigami I, Matsuhashi A, Yamada K, Takei Y.
      Int J Cancer. 2010 Jun 15;126(12):2790-8. PMID:19642105
      A673細胞(ユーイング肉腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Combined functional genome survey of therapeutic targets for hepatocellular carcinoma.
      Satow R, Shitashige M, Kanai Y, Takeshita F, Ojima H, Jigami T, Honda K, Kosuge T, Ochiya T, Hirohashi S, Yamada T.
      Clin Cancer Res. 2010 May 1;16(9):2518-28. PMID:20388846
      KIM-1細胞(肝細胞がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Role of syndecan-1 (CD138) in cell survival of human urothelial carcinoma.
      Shimada K, Nakamura M, De Velasco MA, Tanaka M, Ouji Y, Miyake M, Fujimoto K, Hirao K, Konishi N.
      Cancer Sci. 2010 Jan;101(1):155-60. PMID: 19860843
      KU‐7細胞(膀胱がん細胞)の同所移植モデルマウスへのsiRNA局所投与。
    • Traf2- and Nck-interacting kinase is essential for Wnt signaling and colorectal cancer growth.
      Shitashige M, Satow R, Jigami T, Aoki K, Honda K, Shibata T, Ono M, Hirohashi S, Yamada T.
      Cancer Res. 2010 Jun 15;70(12):5024-33. PMID:20530691
      HCT116細胞(大腸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model.
      Sudo H, Tsuji AB, Sugyo A, Kohda M, Sogawa C, Yoshida C, Harada YN, Hino O, Saga T.
      Genomics. 2010 Apr;95(4):210-6. PMID:20153416
      211H細胞(中皮腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Mutant mouse p53 transgene elevates the chemical induction of tumors that respond to gene silencing with siRNA.
      Tanooka H, Tatsumi K, Tsuji H, Noda Y, Katsube T, Ishii H, Ootsuyama A, Takeshita F, Ochiya T.
      Cancer Gene Ther. 2010 Jan;17(1):1-10. PMID:19557034
      3-methylcholanthreneで誘発した腫瘍塊の皮下移植マウスモデルへのsiRNA局所投与。
    • beta-catenin small interfering RNA successfully suppressed progression of multiple myeloma in a mouse model.
      Ashihara E, Kawata E, Nakagawa Y, Shimazaski C, Kuroda J, Taniguchi K, Uchiyama H, Tanaka R, Yokota A, Takeuchi M, Kamitsuji Y, Inaba T, Taniwaki M, Kimura S, Maekawa T.
      Clin Cancer Res. 2009 Apr 15;15(8):2731-8. PMID: 19351774
      RPMI8226細胞(骨髄腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Amphiregulin is a factor for resistance of glioma cells to cannabinoid-induced apoptosis.
      Lorente M, Carracedo A, Torres S, Natali F, Egia A, Hernández-Tiedra S, Salazar M, Blázquez C, Guzmán M, Velasco G.
      Glia. 2009 Oct;57(13):1374-85. PMID:19229996
      C6.4細胞(神経膠腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Promotion of glioma cell survival by acyl-CoA synthetase 5 under extracellular acidosis conditions.
      Mashima T, Sato S, Sugimoto Y, Tsuruo T, Seimiya H.
      Oncogene. 2009 Jan 8;28(1):9-19. PMID: 18806831
      A1207細胞(神経膠芽腫細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • A novel human AlkB homologue, ALKBH8, contributes to human bladder cancer progression.
      Shimada K, Nakamura M, Anai S, De Velasco M, Tanaka M, Tsujikawa K, Ouji Y, Konishi N.
      Cancer Res. 2009 Apr 1;69(7):3157-64. PMID:19293182
      KU7細胞(膀胱がん細胞)の同所移植モデルマウスへのsiRNA局所投与。
    • Syndecan-1, a new target molecule involved in progression of androgen-independent prostate cancer.
      Shimada K, Nakamura M, De Velasco MA, Tanaka M, Ouji Y, Konishi N.
      Cancer Sci. 2009 Jul;100(7):1248-54. PMID:19432893
      PC-3細胞(前立腺がん細胞の方)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Functional genome screen for therapeutic targets of osteosarcoma.
      Yamaguchi U, Honda K, Satow R, Kobayashi E, Nakayama R, Ichikawa H, Shoji A, Shitashige M, Masuda M, Kawai A, Chuman H, Iwamoto Y, Hirohashi S, Yamada T.
      Cancer Sci. 2009 Dec;100(12):2268-74. PMID:19725836
      MNNG/HOS細胞(骨肉種細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Characterization of the recurrent 8p11-12 amplicon identifies PPAPDC1B, a phosphatase protein, as a new therapeutic target in breast cancer.
      Bernard-Pierrot I, Gruel N, Stransky N, Vincent-Salomon A, Reyal F, Raynal V, Vallot C, Pierron G, Radvanyi F, Delattre O.
      Cancer Res. 2008 Sep 1;68(17):7165-75. PMID:18757432
      ZR-75-1細胞(乳がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • RPN2 gene confers docetaxel resistance in breast cancer.
      Honma K, Iwao-Koizumi K, Takeshita F, Yamamoto Y, Yoshida T, Nishio K, Nagahara S, Kato K, Ochiya T.
      Nat Med. 2008 Sep;14(9):939-48. PMID:18724378
      MCF7-ADR細胞、MDA-MB-231/MDR1(乳がん細胞)の同所移植モデルマウスへのsiRNA局所投与。
    • A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model.
      Iwaki K, Shibata K, Ohta M, Endo Y, Uchida H, Tominaga M, Okunaga R, Kai S, Kitano S.
      Int J Cancer. 2008 Feb 1;122(3):658-63. PMID:17935125
      PANC-1細胞(膵臓がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • ATP citrate lyase: activation and therapeutic implications in non-small cell lung cancer.
      Migita T, Narita T, Nomura K, Miyagi E, Inazuka F, Matsuura M, Ushijima M, Mashima T, Seimiya H, Satoh Y, Okumura S, Nakagawa K, Ishikawa Y.
      Cancer Res. 2008 Oct 15;68(20):8547-54. PMID:18922930
      A549細胞(肺がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Effects of small interfering RNA targeting thymidylate synthase on survival of ACC3 cells from salivary adenoid cystic carcinoma.
      Shirasaki T, Maruya S, Mizukami H, Kakehata S, Kurotaki H, Yagihashi S, Shinkawa H.
      BMC Cancer. 2008 Nov 26;8:348. PMID:19036131
      ACC-3細胞(唾液腺嚢胞がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Heregulin-induced activation of ErbB3 by EGFR tyrosine kinase activity promotes tumor growth and metastasis in melanoma cells.
      Ueno Y, Sakurai H, Tsunoda S, Choo MK, Matsuo M, Koizumi K, Saiki I.
      Int J Cancer. 2008 Jul 15;123(2):340-7. PMID:18398842
      B16‐BL6細胞(メラノーマ細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • New highly potent and specific E6 and E7 siRNAs for treatment of HPV16 positive cervical cancer.
      Yamato K, Yamada T, Kizaki M, Ui-Tei K, Natori Y, Fujino M, Nishihara T, Ikeda Y, Nasu Y, Saigo K, Yoshinouchi M.
      Cancer Gene Ther. 2008 Mar;15(3):140-53. PMID:18157144
      SiHa細胞(子宮頸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.
      Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R.
      Cancer Sci. 2008 Apr;99(4):810-5. PMID:18377429
      HeLa細胞(子宮頸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Nek2 as an effective target for inhibition of tumorigenic growth and peritoneal dissemination of cholangiocarcinoma.
      Kokuryo T, Senga T, Yokoyama Y, Nagino M, Nimura Y, Hamaguchi M.
      Cancer Res. 2007 Oct 15;67(20):9637-42. PMID:17942892
      HuCCT1細胞(胆管がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。また、腹膜播種モデルマウスへの腹腔内投与。
    • Single-injection ornithine decarboxylase-directed antisense therapy using atelocollagen to suppress human cancer growth.
      Nakazawa K, Nemoto T, Hata T, Seyama Y, Nagahara S, Sano A, Itoh H, Nagai Y, Kubota S.
      Cancer. 2007 Mar 1;109(5):993-1002. PMID:17318877
      MKN45細胞(胃がん細胞)、COLO201細胞(大腸がん細胞)、RD細胞(横紋筋肉腫細胞)の皮下腫瘍モデルへのアンチセンスオリゴ局所投与。また、同モデルへのアンチセンスオリゴ腹腔内投与および筋肉内投与。
    • Tumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cells.
      Tazawa H, Tsuchiya N, Izumiya M, Nakagama H.
      Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15472-7. PMID:17875987
      HCT116細胞およびRKO細胞(大腸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Involvement of aminopeptidase N in enhanced chemosensitivity to paclitaxel in ovarian carcinoma in vitro and in vivo.
      Yamashita M, Kajiyama H, Terauchi M, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F.
      Int J Cancer. 2007 May 15;120(10):2243-50. PMID:17266036
      ES‐2細胞(卵巣がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Intratumor injection of small interfering RNA-targeting human papillomavirus 18 E6 and E7 successfully inhibits the growth of cervical cancer.
      Fujii T, Saito M, Iwasaki E, Ochiya T, Takei Y, Hayashi S, Ono A, Hirao N, Nakamura M, Kubushiro K, Tsukazaki K, Aoki D.
      Int J Oncol. 2006 Sep;29(3):541-8. PMID:16865269
      SKG-II細胞(子宮頸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Small interfering RNA targeting epidermal growth factor receptor enhances chemosensitivity to cisplatin, 5-fluorouracil and docetaxel in head and neck squamous cell carcinoma.
      Nozawa H, Tadakuma T, Ono T, Sato M, Hiroi S, Masumoto K, Sato Y.
      Cancer Sci. 2006 Oct;97(10):1115-24. PMID:16984384
      SAS細胞(口腔扁平上皮がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Combinational antitumor effect of siRNA against midkine and paclitaxel on growth of human prostate cancer xenografts.
      Takei Y, Kadomatsu K, Goto T, Muramatsu T.
      Cancer. 2006 Aug 15;107(4):864-73. PMID:16832814
      PC‐3細胞(前立腺がん細胞の方)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • The human papillomavirus E6 and E7 inducible oncogene, hWAPL, exhibits potential as a therapeutic target.
      Kuroda M, Kiyono T, Oikawa K, Yoshida K, Mukai K.
      Br J Cancer. 2005 Jan 31;92(2):290-3. PMID:15655544
      SiHa細胞(子宮頸がん細胞)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Morpholino antisense oligomer targeting human midkine: its application for cancer therapy.
      Takei Y, Kadomatsu K, Yuasa K, Sato W, Muramatsu T.
      Int J Cancer. 2005 Apr 10;114(3):490-7. PMID:15578698
      PC‐3細胞(前立腺がん細胞の方)の皮下腫瘍モデルマウスへのモルフォリノオリゴ局所投与。
    • Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo.
      Minakuchi Y, Takeshita F, Kosaka N, Sasaki H, Yamamoto Y, Kouno M, Honma K, Nagahara S, Hanai K, Sano A, Kato T, Terada M, Ochiya T.
      Nucleic Acids Res. 2004 Jul 22;32(13):e109. PMID:15272050
      NEC8細胞(精巣胚細胞性腫瘍細胞)の同所移植モデルマウスへのsiRNA局所投与。
    • A small interfering RNA targeting vascular endothelial growth factor as cancer therapeutics.
      Takei Y, Kadomatsu K, Yuzawa Y, Matsuo S, Muramatsu T.
      Cancer Res. 2004 May 15;64(10):3365-70. PMID:15150085
      PC‐3細胞(前立腺がん細胞の方)の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Antisense oligodeoxynucleotide against HST-1/FGF-4 suppresses tumorigenicity of an orthotopic model for human germ cell tumor in nude mice.
      Hirai K, Sasaki H, Sakamoto H, Takeshita F, Asano K, Kubota Y, Ochiya T, Terada M.
      J Gene Med. 2003 Nov;5(11):951-7. PMID:14601132
      NEC8細胞(精巣胚細胞性腫瘍細胞)の同所移植モデルマウスへのアンチセンスオリゴ局所投与。
    • 5'-,3'-inverted thymidine-modified antisense oligodeoxynucleotide targeting midkine. Its design and application for cancer therapy.
      Takei Y, Kadomatsu K, Itoh H, Sato W, Nakazawa K, Kubota S, Muramatsu T.
      J Biol Chem. 2002 Jun 28;277(26):23800-6. PMID: 11959856
      CMT-93細胞(大腸がん細胞)の皮下腫瘍モデルマウスへのアンチセンスオリゴ局所投与。
    • Antisense oligodeoxynucleotide targeted to Midkine, a heparin-binding growth factor, suppresses tumorigenicity of mouse rectal carcinoma cells.
      Takei Y, Kadomatsu K, Matsuo S, Itoh H, Nakazawa K, Kubota S, Muramatsu T.
      Cancer Res. 2001 Dec 1;61(23):8486-91. PMID:11731432
      CMT-93細胞(大腸がん細胞)の皮下腫瘍モデルマウスへのアンチセンスオリゴ局所投与。
    • Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13.
      Miyauchi A, Noguchi M, Lei XF, Sakaki M, Kobayashi-Tanabe M, Haraguchi S, Miyazaki A, Kim-Kaneyama JR.
      Sci Rep. 2023 May 8;13(1):7446. PMID: 37156857.
      変形性関節症モデルラットの関節内へのsiRNA局所投与。
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    • Effects of Co-Transfection with Myostatin-Targeting siRNA and ActRIIB-Fc Fusion Protein on Skeletal Muscle Growth.
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    • RasGRP4 is Aberrantly Expressed in the Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis and Controls their Proliferation.
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    • The CXCR4/SDF1 axis improves muscle regeneration through MMP-10 activity.
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    • The Effect of Intra-articular Injection of MicroRNA-210 on Ligament Healing in a Rat Model.
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    • The Contribution of LIGHT (TNFSF14) to the Development of Systemic Sclerosis by Modulating IL-6 and T Helper Type 1 Chemokine Expression in Dermal Fibroblasts.
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    • Elevated Alpha 1(I) to Alpha 2(I) Collagen Ratio in Dermal Fibroblasts Possibly Contributes to Fibrosis in Systemic Sclerosis.
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    • Regulation of skin fibrosis by RALDH1-producing dermal dendritic cells via retinoic acid-mediated regulatory T cell induction: A role in scleroderma.
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