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AteloGene® Local Use “Quick Gelation”（徐放性局所投与用）

概要

AteloGene® Local Useのゲル化速度と核酸導入効果を向上させた改良品！

本製品はマウスへの局所投与により siRNA/miRNA を生体組織中へ導入し、効率よく細胞内へ導入するためのin vivoトランスフェクション試薬です。
「AteloGene®」の主成分であるアテロコラーゲンは、正に荷電しているため、適切な濃度と割合で核酸と混合することにより、 静電的に複合体を形成します。そして、複合体形成により、in vivoでの核酸分解酵素による核酸の分解を防ぐことが可能です 。

アテロコラーゲンとは

用途

核酸の局所投与

特長

  • 「AteloGene®」の主成分であるアテロコラーゲンは、siRNA/miRNAと複合体を形成するため、in vivoでのRNAi効果が長続きします。
  • 「AteloGene®」はアテロコラーゲンから作られているため、無毒性です。
  • 「AteloGene® Local Use」は生体内でゲル化する性質を持っているため、siRNA/miRNAが投与部位に留まり、in vivoでの核酸導入効果を高める徐放効果が得られます。
  • 「AteloGene® Local Use “Quick Gelation”」は投与部位でのゲル形成速度を高めることで、従来品よりもin vivoでの核酸導入効果が向上しています。
 

キットの内容

①AteloGene® Local Use “Quick Gelation”
プレフィルドシリンジ
(調製中のロスも含めて540μLで5回投与分)		 540 μL入り × 3 本
②QGバッファー 1.5 mL × 3本＊
③2mLマイクロチューブ 4本 (予備のチューブ1本を含む)
④18G注射針 8本 (予備の針2本を含む)

＊QGバッファーの容量、包装が変更になりました。キットには1回使い切りタイプ1.5mLが3本含まれております。

キット以外に必要な機器・試薬

  • 1mLディスポーザブルシリンジ
  • 27G注射針
  • siRNA/miRNA(AteloSiLence in vivo 用siRNA/miRNAのようなPAGE精製またはHPLC精製グレード)
  • siRNA/miRNA調製用容器および水(滅菌済み、RNase free)
  • 保冷具(クラッシュアイス、コールドブロックなど)
  • ローテーター(転倒攪拌できるもの、TAITEC RT-5、Bibby scientific SB3など)
  • ピペッターおよびチップ(滅菌済み、RNase free)
  • 高速冷却遠心機
  • 麻酔薬
製品番号 製品 包装 価格
#1490 AteloGene® Local Use “Quick Gelation” 1キット(15回投与分※) ¥75,000
(税込¥82,500)

※200μL/回として算出しているため、対象組織によっては15回以上使用可能な場合もあります。

保管方法：冷蔵（2〜10℃）　本品は研究用試薬です。
代理店への仕切り価格は、家田化学薬品株式会社（03-3816-1871）へご確認ください。

使用例

【AteloGene® Local Use “Quick Gelation”を用いたマウス皮下腫瘍モデルへのsiRNA局所投与】

マウス皮下に移植したデュアルルシフェラーゼ発現メラノーマ細胞に対し、「AteloGene® Local Use “QuickGelation”(QG)」を使用してルシフェラーゼsiRNA(Luc siRNA)を局所投与すると、顕著なルシフェラーゼ発現抑制効果が認められた。

使用事例

  • 使用事例1
  • 使用事例2

  • miR-107 局所投与による腫瘍増殖抑制

    miR-107局所投与による腫瘍増殖抑制   血漿中のmiR-107発現量

    膵臓がんモデルの皮下腫瘍周囲へmiR-107＋AteloGene® を投与（週1回×合計4回）したところ、投与後14、21日目に約60%の腫瘍増殖抑制が認められた。さらに、膵臓がん患者やマウスでは発現が抑制されているmiR-107が、通常マウスと同程度の発現量に上昇した。(Scientifi­c reports. 2017 7(1):5708.)
    （データ提供：京都府立医科大学/ 京都第一赤十字病院 小松周平准教授, 今村泰輔先生）

  • RasGRP-4 siRNA 局所投与による関節炎の改善

    RasGRP発現量のグラフと画像関節炎スコアが大幅に改善

    コラーゲン誘導関節炎モデルのラット足首関節内へR a s G R P – 4 s i R N A ＋AteloGene® を投与（関節炎誘導後14日目に1回）したところ、誘導後35日目にRasGRP-4タンパク質発現が約80%抑制された。さらに、関節破壊の進行が抑制され、関節炎スコアなどが大幅に改善された。(Arthritis Rheumatol. 2015 67(2):396-407.)
    （データ提供：北海道大学　河野 通仁助教）

参考文献

  • がん関連
  • 運動器
  • 皮膚
  • 循環器
  • 脳神経
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      H2373細胞（中皮腫細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。また、同所移植モデルマウス胸腔内へのmiRNA局所投与。
    • MCL1 inhibition enhances the therapeutic effect of MEK inhibitors in KRAS-mutant lung adenocarcinoma cells.
      Tada M, Sumi T, Tanaka Y, Hirai S, Yamaguchi M, Miyajima M, Niki T, Takahashi H, Watanabe A, Sakuma Y.
      Lung Cancer. 2019 Jul;133:88-95. PMID: 31200834.
      H441細胞（肺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • MicroRNA-8073: Tumor suppressor and potential therapeutic treatment.
      Mizoguchi A, Takayama A, Arai T, Kawauchi J, Sudo H.
      PLoS One. 2018 Dec 27;13(12):e0209750. PMID: 30589909 .
      HCT116細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Screening for long noncoding RNAs associated with oral squamous cell carcinoma reveals the potentially oncogenic actions of DLEU1.
      Nishiyama K, Maruyama R, Niinuma T, Kai M, Kitajima H, Toyota M, Hatanaka Y, Igarashi T, Kobayashi JI, Ogi K, Dehari H, Miyazaki A, Yorozu A, Yamamoto E, Idogawa M4, Sasaki Y, Sugai T, Tokino T, Hiratsuka H, Suzuki H.
      Cell Death Dis. 2018 Aug 1;9(8):826. PMID: 30069008
      HSC-3細胞（口腔扁平上皮がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Survivin knockdown induces senescence in TTF‑1-expressing, KRAS-mutant lung adenocarcinomas.
      Sumi T, Hirai S, Yamaguchi M, Tanaka Y, Tada M, Yamada G, Hasegawa T, Miyagi Y, Niki T, Watanabe A, Takahashi H, Sakuma Y.
      Int J Oncol. 2018 Jul;53(1):33-46. PMID: 29658609
      H358細胞（肺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-3140 suppresses tumor cell growth by targeting BRD4 via its coding sequence and downregulates the BRD4-NUT fusion oncoprotein.
      Tonouchi E, Gen Y, Muramatsu T, Hiramoto H, Tanimoto K, Inoue J, Inazawa J.
      Sci Rep. 2018 Mar 14;8(1):4482. PMID: 29540837
      MIAPaCa2細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Depleted tumor suppressor miR-107 in plasma relates to tumor progression and is a novel therapeutic target in pancreatic cancer
      Taisuke Imamura, Shuhei Komatsu, Daisuke Ichikawa, Mahito Miyamae, Wataru Okajima, Takuma Ohashi, Jun Kiuchi, Keiji Nishibeppu, Hirotaka Konishi, Atsushi Shiozaki, Ryo Morimura, Hisashi Ikoma, Toshiya Ochiai, Kazuma Okamoto, Hiroki Taniguchi, Eigo Otsuji
      Sci Rep. 2017 Jul 18;7(1):5708. PMID: 28720759
      MIAPaCa2細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.
      Kato K, Mukudai Y, Motohashi H, Ito C, Kamoshida S, Shimane T, Kondo S, Shirota T.
      Int J Oncol. 2017 May;50(5):1634-1646. PMID: 28339026
      SAS細胞（口腔扁平上皮がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Ephrin-B2 reverse signaling regulates progression and lymph node metastasis of oral squamous cell carcinoma.
      Sasabe E, Tomomura A, Tomita R, Sento S, Kitamura N, Yamamoto T.
      PLoS One. 2017 Nov 30;12(11):e0188965. PMID: 29190834
      SAS-L1細胞（口腔扁平上皮がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。また、同所移植モデルマウスへのsiRNA局所投与。
    • Radiation-inducible miR-770-5p sensitizes tumors to radiation through direct targeting of PDZ-binding kinase.
      Lee HC, Her NG, Kang D, Jung SH, Shin J, Lee M, Bae IH, Kim YN, Park HJ, Ko YG, Lee JS
      Cell Death Dis. 2017 Mar 23;8(3):e2693. PMID: 28333152
      HCT116細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • The microRNA miR-516a-3p regulates the Wnt pathway by targeting extracellular sulfatase 1
      in human scirrhous gastric cancers: Anti-metastatic therapy via miRNA-based medicine
      Yoshifumi Takei, Akiko Suzuki, Keichiro Mihara, Kazuyoshi Yanagihara
      Medical Research Archives, Vol. 5, Issue 7, July 2017
      58As9細胞（スキルス胃がん細胞）の同所移植モデルマウスへのsiRNA局所投与
    • ANKRD22 promotes progression of non-small cell lung cancer through transcriptional up-regulation of E2F1.
      Yin J, Fu W, Dai L, Jiang Z, Liao H, Chen W, Pan L, Zhao J.
      Sci Rep. 2017 Jun 30;7(1):4430. PMID: 28667340
      H1299細胞（肺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Oncogenic role of rab escort protein 1 through EGFR and STAT3 pathway.
      Yun UJ, Sung JY, Park SY, Ye SK, Shim J, Lee JS, Hibi M, Bae YK, Kim YN.
      Cell Death Dis. 2017 Feb 23;8(2):e2621. PMID: 28230863
      A431細胞（類表皮がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Pharmacological regulation of bladder cancer by miR-130 family seed-targeting LNA.
      Egawa H, Jingushi K, Hirono T, Hirose R, Nakatsuji Y, et al.
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      5637細胞（膀胱がん細胞）の皮下腫瘍モデルマウスへのLNA局所投与。
    • Nek2 siRNA therapy using a portal venous port-catheter system for liver metastasis in pancreatic cancer.
      Kokuryo T, Hibino S, Suzuki K, Watanabe K, Yokoyama Y, Nagino M, Senga T, Hamaguchi M.
      Cancer Sci. 2016 Sep;107(9):1315-20. PMID: 27316377
      KLM1細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • A therapeutic trial of human melanomas with combined small interfering RNAs targeting adaptor molecules p130Cas and paxillin activated under expression of ganglioside GD3.
      Makino Y, Hamamura K, Takei Y, Bhuiyan RH, Ohkawa Y, Ohmi Y, Nakashima H, Furukawa K, Furukawa K.
      Biochim Biophys Acta. 2016 Aug;1860(8):1753-63. PMID: 27068854
      メラノーマ細胞の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Docetaxel induced-JNK2/PHD1 signaling pathway increases degradation of HIF-1α and causes cancer cell death under hypoxia.
      Oh ET, Kim CW, Kim SJ, Lee JS, Hong SS, Park HJ.
      Sci Rep. 2016 Jun 6;6:27382. PMID: 27263528
      MDA-MB-231細胞（乳がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Unfavorable neuroblastoma prognostic factor NLRR2 inhibits cell differentiation by transcriptional induction through JNK pathway.
      Sheikh A, Takatori A, Hossain MS, Hasan MK, Tagawa M, Nagase H, Nakagawara A.
      Cancer Sci. 2016 Sep;107(9):1223-32. PMID: 27357360
      SK‐N‐BE細胞（神経芽細胞腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Regulation of SLD5 gene expression by miR-370 during acute growth of cancer cells.
      Yamane K, Naito H, Wakabayashi T, Yoshida H, Muramatsu F, Iba T, Kidoya H, Takakura N.
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      T24細胞（膀胱がん細胞）の皮下腫瘍モデルマウスへのmiRNAおよびsiRNA局所投与。
    • Usefulness of microRNA‑375 as a prognostic and therapeutic tool in esophageal squamous cell carcinoma.
      Isozaki, Y., Hoshino, I., Akutsu, Y., Hanari, N., Mori, M., Nishimori, T., Murakami, K., Akanuma, N., Takeshita, N., Maruyama, T., Toyozumi, T., Takahashi, M., Suito, H., & Matsubara, H.
      Int J Oncol. 2015 Mar;46(3):1059-66. PMID: 25501018
      T.Tn細胞およびTE-2細胞（食道扁平上皮がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Arl4c expression in colorectal and lung cancers promotes tumorigenesis and may represent a novel therapeutic target.
      Fujii S, Matsumoto S, Nojima S, Morii E, Kikuchi A.
      Oncogene. 2015 Sep 10;34(37):4834-44. PMID: 25486429
      HCT116細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • MiR-634 activates the mitochondrial apoptosis pathway and enhances chemotherapy-induced cytotoxicity.
      Fujiwara N, Inoue J, Kawano T, Tanimoto K, Kozaki KI, Inazawa J.
      Cancer Res. 2015 Sep 15;75(18):3890-901. PMID: 26216549
      KYSE170細胞（食道扁平上皮がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • RFPL4A increases the G1 population and decreases sensitivity to chemotherapy in human colorectal cancer cells.
      Naito A, Yamamoto H, Kagawa Y, Naito Y, Okuzaki D, Otani K, Iwamoto Y, Maeda S, Kikuta J, Nishikawa K, Uemura M, Nishimura J, Hata T, Takemasa I, Mizushima T, Ishii H, Doki Y, Mori M, Ishii M.
      J Biol Chem. 2015 Mar 6;290(10):6326-37. PMID: 25605732
      HCT116細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Efficient inhibition of tumor angiogenesis and growth by a synthetic peptide blocking S100A4-methionine aminopeptidase 2 interaction.
      Ochiya T, Takenaga K, Asagiri M, Nakano K, Satoh H, Watanabe T,
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      Mol Ther Methods Clin Dev. 2015 Apr 1;2:15008. PMID: 26029719
      PC-3M-Luc細胞（前立腺がん細胞）の皮下腫瘍モデルマウスへのペプチド局所投与。
    • Ubiquilin 2 enhances osteosarcoma progression through resistance to hypoxic stress.
      Tsukamoto S, Shimada K, Honoki K, Kido A, Akahane M, Tanaka Y, Konishi N.
      Oncol Rep. 2015 Apr;33(4):1799-806. PMID: 25672654
      COS1NR細胞（骨肉腫細胞）の皮下腫瘍モデルラットへのsiRNA局所投与。
    • APOBEC3B-Mediated Cytidine Deamination Is Required for Estrogen Receptor Action in Breast Cancer.
      Periyasamy M, Patel H, Lai CF, Nguyen VT, Nevedomskaya E, Harrod A, Russell R, Remenyi J, Ochocka AM, Thomas RS, Fuller-Pace F, Győrffy B, Caldas C, Navaratnam N, Carroll JS, Zwart W, Coombes RC, Magnani L, Buluwela L, Ali S.
      Cell Rep. 2015 Oct 6;13(1):108-21. PMID: 26411678
      MCF-7細胞（乳がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Aurora kinase A has a significant role as a therapeutic target and clinical biomarker in endometrial cancer.
      Umene K, Yanokura M, Banno K, Irie H, Adachi M, Iida M, Nakamura K, Nogami Y, Masuda K, Kobayashi Y, Tominaga E, Aoki D.
      Int J Oncol. 2015 Apr;46(4):1498-506. PMID: 25625960
      HEC-1B細胞（子宮体がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Glyoxalase I is differentially expressed in cutaneous neoplasms and contributes to the progression of squamous cell carcinoma.
      Zou XY, Ding D, Zhan N, Liu XM, Pan C, Xia YM.
      J Invest Dermatol. 2015 Feb;135(2):589-98. PMID: 25184957
      SCC-13細胞（扁平上皮がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • RNA interference against cancer/testis genes identifies DUSP21 as a potential therapeutic target in human hepatocellular carcinoma.
      Deng Q, Li KY, Chen H, Dai JH, Zhai YY, Wang Q, Li N, Wang YP, Han ZG.
      Hepatology. 2014 Feb;59(2):518-30. PMID: 23929653
      MHCC‐97H（肝細胞がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • MicroRNAs cooperatively inhibit a network of tumor suppressor genes to promote pancreatic tumor growth and progression.
      Frampton AE, Castellano L, Colombo T, Giovannetti E, Krell J, Jacob J, Pellegrino L, Roca-Alonso L, Funel N, Gall TM, De Giorgio A, Pinho FG, Fulci V, Britton DJ, Ahmad R, Habib NA, Coombes RC, Harding V, Knösel T, Stebbing J, Jiao LR.
      Gastroenterology. 2014 Jan;146(1):268-77.e18. PMID: 24120476
      MIAPaca-2細胞およびPANC-1細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのmiRNA inhibitor局所投与。
    • Guanine nucleotide-binding protein 1 is one of the key molecules contributing to cancer cell radioresistance.
      Fukumoto M, Amanuma T, Kuwahara Y, Shimura T, Suzuki M, Mori S, Kumamoto H, Saito Y, Ohkubo Y, Duan Z, Sano K, Oguchi T, Kainuma K, Usami S, Kinoshita K, Lee I, Fukumoto M.
      Cancer Sci. 2014 Oct;105(10):1351-9. PMID: 25098609
      SAS細胞およびSAS-R1細胞（口腔扁平上皮がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Paired box 2 upregulates androgen receptor gene expression in androgen-independent prostate cancer.
      Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Hongo F, Kamoi K, Okihara K, Kawauchi A, Miki T.
      FEBS J. 2014 Oct;281(19):4506-18. PMID: 25132193
      22Rv1細胞（前立腺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • High expression of protein tyrosine kinase 7 significantly associates with invasiveness and poor prognosis in intrahepatic cholangiocarcinoma.
      Jin J, Ryu HS, Lee KB, Jang JJ.
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      HuCCT細胞（胆管がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma.
      Migita K, Sho M, Shimada K, Yasuda S, Yamato I, Takayama T, Matsumoto S, Wakatsuki K, Hotta K, Tanaka T, Ito M, Konishi N, Nakajima Y.
      Cancer. 2014 Mar 15;120(6):808-17. PMID: 24249528
      Colon-26細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Targeting CB2-GPR55 Receptor Heteromers Modulates Cancer Cell Signaling.
      Moreno E, Andradas C, Medrano M, Caffarel MM, Pérez-Gómez E, Blasco-Benito S, Gómez-Cañas M, Pazos MR, Irving AJ, Lluís C, Canela EI, Fernández-Ruiz J, Guzmán M, McCormick PJ, Sánchez C.
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      T98G（神経膠芽腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-296/Scribble axis is deregulated in human breast cancer and miR-296 restoration reduces tumour growth in vivo.
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      MDA-MB-231細胞（乳がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • AFAP1L1, a novel associating partner with vinculin, modulates cellular morphology and motility, and promotes the progression of colorectal cancers.
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      RKO細胞、LoVo細胞、SW480細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • BMP4/Thrombospondin-1 loop paracrinically inhibits tumor angiogenesis and suppresses the growth of solid tumors.
      Tsuchida R, Osawa T, Wang F, Nishii R, Das B, Tsuchida S, Muramatsu M, Takahashi T, Inoue T, Wada Y, Minami T, Yuasa Y, Shibuya M.
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      HeLa細胞（子宮頸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Potential of tumor-suppressive miR-596 targeting LGALS3BP as a therapeutic agent in oral cancer.
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      NA細胞（口腔扁平上皮がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Y-box binding protein-1 regulates cell proliferation and is associated with clinical outcomes of osteosarcoma.
      Fujiwara-Okada Y, Matsumoto Y, Fukushi J, Setsu N, Matsuura S, Kamura S, Fujiwara T, Iida K, Hatano M, Nabeshima A, Yamada H, Ono M, Oda Y, Iwamoto Y.
      Br J Cancer. 2013 Mar 5;108(4):836-47. PMID: 23462806
      MNNG細胞（骨肉腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-146a enhances the oncogenicity of oral carcinoma by concomitant targeting of the IRAK1, TRAF6 and NUMB genes.
      Hung PS, Liu CJ, Chou CS, Kao SY, Yang CC, Chang KW, Chiu TH, Lin SC.
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    • Ubiquitin C-terminal Hydrolase L1 (UCH-L1) Acts as a Novel Potentiator of Cyclin-dependent Kinases to Enhance Cell Proliferation, Independent of its Hydrolase Activity.
      Kabuta T, Mitsui T, Takahashi M, Fujiwara Y, Kabuta C, Konya C, Tsuchiya Y, Hatanaka Y, Uchida K, Hohjoh H, Wada K.
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      MCF7細胞（乳がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Cell cycle-dependent Rho GTPase activity dynamically regulates cancer cell motility and invasion in vivo.
      Kagawa Y, Matsumoto S, Kamioka Y, Mimori K, Naito Y, Ishii T, Okuzaki D, Nishida N, Maeda S, Naito A, Kikuta J, Nishikawa K, Nishimura J, Haraguchi N, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Ishii H, Doki Y, Matsuda M, Kikuchi A, Mori M, Ishii M.
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      HCT116細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • In vivo delivery of siRNA targeting vasohibin-2 decreases tumor angiogenesis and suppresses tumor growth in ovarian cancer.
      Koyanagi T, Suzuki Y, Saga Y, Machida S, Takei Y, Fujiwara H, Suzuki M, Sato Y.
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      DISS細胞およびSKOV‐3細胞（卵巣がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Targeting the Vav3 oncogene enhances docetaxel-induced apoptosis through the inhibition of androgen receptor phosphorylation in LNCaP prostate cancer cells under chronic hypoxia.
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      LNCaPH細胞（前立腺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • MicroRNA-196a is a putative diagnostic biomarker and therapeutic target for laryngeal cancer.
      Saito K, Inagaki K, Kamimoto T, Ito Y, Sugita T, Nakajo S, Hirasawa A, Iwamaru A, Ishikura T, Hanaoka H, Okubo K, Onozaki T, Zama T.
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      JHU-011細胞（喉頭がん細胞）の同所移植モデルマウスへのmiRNA inhibitor局所投与。
    • YB-1 suppression induces STAT3 proteolysis and sensitizes renal cancer to interferon-α.
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    • Temozolomide suppresses MYC via activation of TAp63 to inhibit progression of human glioblastoma.
      Yamaki T, Suenaga Y, Iuchi T, Alagu J, Takatori A, Itami M, Araki A, Ohira M, Inoue M, Kageyama H, Yokoi S, Saeki N, Nakagawara A.
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      U87MG細胞（神経膠芽腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Plasma membrane-associated sialidase (NEU3) regulates progression of prostate cancer to androgen-independent growth through modulation of androgen receptor signaling.
      Kawamura S, Sato I, Wada T, Yamaguchi K, Li Y, Li D, Zhao X, Ueno S, Aoki H, Tochigi T, Kuwahara M, Kitamura T, Takahashi K, Moriya S, Miyagi T.
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      PC-3細胞（前立腺がん細胞の方）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Wig1 prevents cellular senescence by regulating p21 mRNA decay through control of RISC recruitment.
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      H460細胞（肺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Anti-tumor effect of AlkB homolog 3 knockdown in hormone-independent prostate cancer cells.
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      DU145細胞（前立腺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Up-regulation of endogenous PML induced by a combination of interferon-beta and temozolomide enhances p73/YAP-mediated apoptosis in glioblastoma.
      Okazaki T, Kageji T, Kuwayama K, Kitazato KT, Mure H, Hara K, Morigaki R, Mizobuchi Y, Matsuzaki K, Nagahiro S.
      Cancer Lett. 2012 Oct 28;323(2):199-207. PMID:22542810
      神経膠芽腫細胞の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • D-amino acid oxidase gene therapy sensitizes glioma cells to the antiglycolytic effect of 3-bromopyruvate.
      El Sayed SM, Abou El-Magd RM, Shishido Y, Chung SP, Sakai T, Watanabe H, Kagami S, Fukui K.
      Cancer Gene Ther. 2012 Jan;19(1):1-18. PMID:21921941
      C6細胞（神経膠腫細胞）の皮下腫瘍モデルラットへのプラスミドDNA局所投与。
    • ALKBH3 contributes to survival and angiogenesis of human urothelial carcinoma cells through NADPH oxidase and Tweak/Fn14/VEGF signals.
      Shimada K, Fujii T, Anai S, Fujimoto K, Konishi N.
      Clin Cancer Res. 2012 Oct 1;18(19):5247-55. PMID:22850567
      KU7-GFP細胞（膀胱がん細胞）のCAMアッセイ時や同所移植モデルマウスへのsiRNA局所投与。
    • ZDHHC8 knockdown enhances radiosensitivity and suppresses tumor growth in a mesothelioma mouse model.
      Sudo H, Tsuji AB, Sugyo A, Ogawa Y, Sagara M, Saga T.
      Cancer Sci. 2012 Feb;103(2):203-9. PMID:22017350
      211H細胞（中皮種細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Therapeutic Potential of SOX2 Inhibition for Embryonal Carcinoma.
      Ushida H, Chano T, Minami K, Kita H, Kawakami T, Okabe H, Okada Y, Okamoto K.
      J Urol. 2012 May;187(5):1876-81. PMID:22425046
      NEC8細胞（精巣胚細胞性腫瘍細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • NKX2-1/TITF1/TTF-1-Induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma.
      Yamaguchi T, Yanagisawa K, Sugiyama R, Hosono Y, Shimada Y, Arima C, Kato S, Tomida S, Suzuki M, Osada H, Takahashi T.
      Cancer Cell. 2012 Mar 20;21(3):348-61. PMID:22439932
      H1975細胞（肺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Nestin as a novel therapeutic target for pancreatic cancer via tumor angiogenesis.
      Yamahatsu K1, Matsuda Y, Ishiwata T, Uchida E, Naito Z.
      Int J Oncol. 2012 May;40(5):1345-57. PMID: 22246533
      KLM-1 細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • PCA-1/ALKBH3 contributes to pancreatic cancer by supporting apoptotic resistance and angiogenesis.
      Yamato I, Sho M, Shimada K, Hotta K, Ueda Y, Yasuda S, Shigi N, Konishi N, Tsujikawa K, Nakajima Y.
      Cancer Res. 2012 Sep 15;72(18):4829-39. PMID:22826605
      PANC-1細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。また、同所移植モデルへのsiRNA局所投与。
    • The orphan G protein-coupled receptor GPR55 promotes cancer cell proliferation via ERK.
      Andradas C, Caffarel MM, Pérez-Gómez E, Salazar M, Lorente M, Velasco G, Guzmán M, Sánchez C.
      Oncogene. 2011 Jan 13;30(2):245-52. PMID:20818416
      T98G細胞（神経膠芽腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • RECQL1 and WRN proteins are potential therapeutic targets in head and neck squamous cell carcinoma.
      Arai A, Chano T, Futami K, Furuichi Y, Ikebuchi K, Inui T, Tameno H, Ochi Y, Shimada T, Hisa Y, Okabe H.
      Cancer Res. 2011 Jul 1;71(13):4598-607. PMID:21571861
      FaDu細胞（咽頭がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • GEP100-Arf6-AMAP1-cortactin pathway frequently used in cancer invasion is activated by VEGFR2 to promote angiogenesis.
      Hashimoto A, Hashimoto S, Ando R, Noda K, Ogawa E, Kotani H, Hirose M, Menju T, Morishige M, Manabe T, Toda Y, Ishida S, Sabe H.
      PLoS One. 2011;6(8):e23359. PMID:21858086
      MDA-MB-231細胞（乳がん細胞）が播種されたangioreacter tube内へのsiRNA局所投与。
    • Transient silencing of galectin-3 expression promotes both in vitro and in vivo drug-induced apoptosis of human pancreatic carcinoma cells.
      Kobayashi T, Shimura T, Yajima T, Kubo N, Araki K, Wada W, Tsutsumi S, Suzuki H, Kuwano H, Raz A.
      Clin Exp Metastasis. 2011 Apr;28(4):367-76. PMID:21331750
      MIA-PaCa2細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Stimulation of the midkine/ALK axis renders glioma cells resistant to cannabinoid antitumoral action.
      Lorente M, Torres S, Salazar M, Carracedo A, Hernández-Tiedra S, Rodríguez-Fornés F, García-Taboada E, Meléndez B, Mollejo M, Campos-Martín Y, Lakatosh SA, Barcia J, Guzmán M, Velasco G.
      Cell Death Cell Death Differ. 2011 Jun;18(6):959-73. PMID:21233844
      U-87MG細胞およびT98G細胞（神経膠芽腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-135b mediates NPM-ALK-driven oncogenicity and renders IL-17-producing immunophenotype to anaplastic large cell lymphoma.
      Matsuyama H, Suzuki HI, Nishimori H, Noguchi M, Yao T, Komatsu N, Mano H, Sugimoto K, Miyazono K.
      Blood. 2011 Dec 22;118(26):6881-92. PMID:22042699
      KARPAS 299細胞（未分化大細胞リンパ腫細胞）の皮下腫瘍モデルマウスへのLNA局所投与。
    • ZNF689 suppresses apoptosis of hepatocellular carcinoma cells through the down-regulation of Bcl-2 family members.
      Shigematsu S, Fukuda S, Nakayama H, Inoue H, Hiasa Y, Onji M, Higashiyama S.
      Exp Cell Res. 2011 Aug 1;317(13):1851-9. PMID:21624362
      HepG2細胞（肝細胞がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • The metastasis-associated microRNA miR-516a-3p is a novel therapeutic target for inhibiting peritoneal dissemination of human scirrhous gastric cancer.
      Takei Y, Takigahira M, Mihara K, Tarumi Y, Yanagihara K.
      Cancer Res. 2011 Feb 15;71(4):1442-1453. PMID:21169410
      44As3細胞（スキルス胃がん細胞）の同所移植モデルマウスへのmiRNA発現べクター局所投与。
    • Synthetic siRNA targeting the breakpoint of EWS/Fli-1 inhibits growth of Ewing sarcoma xenografts in a mouse model.
      Takigami I, Ohno T, Kitade Y, Hara A, Nagano A, Kawai G, Saitou M, Matsuhashi A, Yamada K, Shimizu K.
      Int J Cancer. 2011 Jan 1;128(1):216-26. PMID:20648560
      TC‐135細胞（ユーイング肉腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • ALKBH3, a human AlkB homologue, contributes to cell survival in human non-small-cell lung cancer.
      Tasaki M, Shimada K, Kimura H, Tsujikawa K, Konishi N.
      Br J Cancer. 2011 Feb 15;104(4):700-6. PMID:21285982
      A549細胞およびRERF-LC-AI細胞（肺がん細胞）の腹膜播種モデルマウスへのsiRNA腹腔内投与。
    • Suppression of SOCS3 increases susceptibility of renal cell carcinoma to interferon-α.
      Tomita S, Ishibashi K, Hashimoto K, Sugino T, Yanagida T, Kushida N, Shishido K, Aikawa K, Sato Y, Suzutani T, Yamaguchi O.
      Cancer Sci. 2011 Jan;102(1):57-63. PMID:21054677
      786‐O細胞（腎細胞がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-152 is a tumor suppressor microRNA that is silenced by DNA hypermethylation in endometrial cancer.
      Tsuruta T, Kozaki K, Uesugi A, Furuta M, Hirasawa A, Imoto I, Susumu N, Aoki D, Inazawa J.
      Cancer Res. 2011 Oct 15;71(20):6450-62. PMID:21868754
      HEC-1細胞（子宮体がん細胞）の皮下腫瘍モデルマウスへのmiRNA局所投与。
    • Anti-tumoral action of cannabinoids on hepatocellular carcinoma: role of AMPK-dependent activation of autophagy.
      Vara D, Salazar M, Olea-Herrero N, Guzmán M, Velasco G, Díaz-Laviada I.
      Cell Death Differ. 2011 Jul;18(7):1099-111. PMID:21475304
      HepG2細胞（肝細胞がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • The cancer/testis antigen prostate-associated gene 4 (PAGE4) is a highly intrinsically disordered protein.
      Zeng Y, He Y, Yang F, Mooney S, Getzenberg R, Orban J, Kulkarni P.　
      J Biol Chem. 2011 Apr 22;286(16):13985-94. PMID:21357425
      LNCaP細胞（前立腺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Atelocollagen-delivered siRNA targeting the FABP5 gene as an experimental therapy for prostate cancer in mouse xenografts.
      Forootan SS, Bao ZZ, Forootan FS, Kamalian L, Zhang Y, Bee A, Foster CS, Ke Y.
      Int J Oncol. 2010 Jan;36(1):69-76. PMID:19956834
      PC3-M細胞（前立腺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Shank-interacting protein-like 1 promotes tumorigenesis via PTEN inhibition in human tumor cells.
      He L, Ingram A, Rybak AP, Tang D.
      J Clin Invest. 2010 Jun 1;120(6):2094-108. PMID:20458142
      HeLa細胞（子宮頸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • miR-31 ablates expression of the HIF regulatory factor FIH to activate the HIF pathway in head and neck carcinoma.
      Liu CJ, Tsai MM, Hung PS, Kao SY, Liu TY, Wu KJ, Chiou SH, Lin SC, Chang KW.
      Cancer Res. 2010 Feb 15;70(4):1635-44. PMID:20145132
      SAS細胞（口腔扁平上皮がん細胞）の皮下腫瘍モデルマウスへのLNA局所投与。
    • Cyclin K and cyclin D1b are oncogenic in myeloma cells.
      Marsaud V, Tchakarska G, Andrieux G, Liu JM, Dembele D, Jost B, Wdzieczak-Bakala J, Renoir JM, Sola B.
      Mol Cancer. 2010 May 10;9:103. PMID:20459741
      LP-1細胞（骨髄腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • EWS/Fli-1 chimeric fusion gene upregulates vascular endothelial growth factor-A.
      Nagano A, Ohno T, Shimizu K, Hara A, Yamamoto T, Kawai G, Saitou M, Takigami I, Matsuhashi A, Yamada K, Takei Y.
      Int J Cancer. 2010 Jun 15;126(12):2790-8. PMID:19642105
      A673細胞（ユーイング肉腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Combined functional genome survey of therapeutic targets for hepatocellular carcinoma.
      Satow R, Shitashige M, Kanai Y, Takeshita F, Ojima H, Jigami T, Honda K, Kosuge T, Ochiya T, Hirohashi S, Yamada T.
      Clin Cancer Res. 2010 May 1;16(9):2518-28. PMID:20388846
      KIM-1細胞（肝細胞がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Role of syndecan-1 (CD138) in cell survival of human urothelial carcinoma.
      Shimada K, Nakamura M, De Velasco MA, Tanaka M, Ouji Y, Miyake M, Fujimoto K, Hirao K, Konishi N.
      Cancer Sci. 2010 Jan;101(1):155-60. PMID: 19860843
      KU‐7細胞（膀胱がん細胞）の同所移植モデルマウスへのsiRNA局所投与。
    • Traf2- and Nck-interacting kinase is essential for Wnt signaling and colorectal cancer growth.
      Shitashige M, Satow R, Jigami T, Aoki K, Honda K, Shibata T, Ono M, Hirohashi S, Yamada T.
      Cancer Res. 2010 Jun 15;70(12):5024-33. PMID:20530691
      HCT116細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model.
      Sudo H, Tsuji AB, Sugyo A, Kohda M, Sogawa C, Yoshida C, Harada YN, Hino O, Saga T.
      Genomics. 2010 Apr;95(4):210-6. PMID:20153416
      211H細胞（中皮腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Mutant mouse p53 transgene elevates the chemical induction of tumors that respond to gene silencing with siRNA.
      Tanooka H, Tatsumi K, Tsuji H, Noda Y, Katsube T, Ishii H, Ootsuyama A, Takeshita F, Ochiya T.
      Cancer Gene Ther. 2010 Jan;17(1):1-10. PMID:19557034
      3-methylcholanthreneで誘発した腫瘍塊の皮下移植マウスモデルへのsiRNA局所投与。
    • beta-catenin small interfering RNA successfully suppressed progression of multiple myeloma in a mouse model.
      Ashihara E, Kawata E, Nakagawa Y, Shimazaski C, Kuroda J, Taniguchi K, Uchiyama H, Tanaka R, Yokota A, Takeuchi M, Kamitsuji Y, Inaba T, Taniwaki M, Kimura S, Maekawa T.
      Clin Cancer Res. 2009 Apr 15;15(8):2731-8. PMID: 19351774
      RPMI8226細胞（骨髄腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Amphiregulin is a factor for resistance of glioma cells to cannabinoid-induced apoptosis.
      Lorente M, Carracedo A, Torres S, Natali F, Egia A, Hernández-Tiedra S, Salazar M, Blázquez C, Guzmán M, Velasco G.
      Glia. 2009 Oct;57(13):1374-85. PMID:19229996
      C6.4細胞（神経膠腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Promotion of glioma cell survival by acyl-CoA synthetase 5 under extracellular acidosis conditions.
      Mashima T, Sato S, Sugimoto Y, Tsuruo T, Seimiya H.
      Oncogene. 2009 Jan 8;28(1):9-19. PMID: 18806831
      A1207細胞（神経膠芽腫細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • A novel human AlkB homologue, ALKBH8, contributes to human bladder cancer progression.
      Shimada K, Nakamura M, Anai S, De Velasco M, Tanaka M, Tsujikawa K, Ouji Y, Konishi N.
      Cancer Res. 2009 Apr 1;69(7):3157-64. PMID:19293182
      KU7細胞（膀胱がん細胞）の同所移植モデルマウスへのsiRNA局所投与。
    • Syndecan-1, a new target molecule involved in progression of androgen-independent prostate cancer.
      Shimada K, Nakamura M, De Velasco MA, Tanaka M, Ouji Y, Konishi N.
      Cancer Sci. 2009 Jul;100(7):1248-54. PMID:19432893
      PC-3細胞（前立腺がん細胞の方）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Functional genome screen for therapeutic targets of osteosarcoma.
      Yamaguchi U, Honda K, Satow R, Kobayashi E, Nakayama R, Ichikawa H, Shoji A, Shitashige M, Masuda M, Kawai A, Chuman H, Iwamoto Y, Hirohashi S, Yamada T.
      Cancer Sci. 2009 Dec;100(12):2268-74. PMID:19725836
      MNNG/HOS細胞（骨肉種細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Characterization of the recurrent 8p11-12 amplicon identifies PPAPDC1B, a phosphatase protein, as a new therapeutic target in breast cancer.
      Bernard-Pierrot I, Gruel N, Stransky N, Vincent-Salomon A, Reyal F, Raynal V, Vallot C, Pierron G, Radvanyi F, Delattre O.
      Cancer Res. 2008 Sep 1;68(17):7165-75. PMID:18757432
      ZR-75-1細胞（乳がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • RPN2 gene confers docetaxel resistance in breast cancer.
      Honma K, Iwao-Koizumi K, Takeshita F, Yamamoto Y, Yoshida T, Nishio K, Nagahara S, Kato K, Ochiya T.
      Nat Med. 2008 Sep;14(9):939-48. PMID:18724378
      MCF7-ADR細胞、MDA-MB-231/MDR1（乳がん細胞）の同所移植モデルマウスへのsiRNA局所投与。
    • A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model.
      Iwaki K, Shibata K, Ohta M, Endo Y, Uchida H, Tominaga M, Okunaga R, Kai S, Kitano S.
      Int J Cancer. 2008 Feb 1;122(3):658-63. PMID:17935125
      PANC-1細胞（膵臓がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • ATP citrate lyase: activation and therapeutic implications in non-small cell lung cancer.
      Migita T, Narita T, Nomura K, Miyagi E, Inazuka F, Matsuura M, Ushijima M, Mashima T, Seimiya H, Satoh Y, Okumura S, Nakagawa K, Ishikawa Y.
      Cancer Res. 2008 Oct 15;68(20):8547-54. PMID:18922930
      A549細胞（肺がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Effects of small interfering RNA targeting thymidylate synthase on survival of ACC3 cells from salivary adenoid cystic carcinoma.
      Shirasaki T, Maruya S, Mizukami H, Kakehata S, Kurotaki H, Yagihashi S, Shinkawa H.
      BMC Cancer. 2008 Nov 26;8:348. PMID:19036131
      ACC-3細胞（唾液腺嚢胞がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Heregulin-induced activation of ErbB3 by EGFR tyrosine kinase activity promotes tumor growth and metastasis in melanoma cells.
      Ueno Y, Sakurai H, Tsunoda S, Choo MK, Matsuo M, Koizumi K, Saiki I.
      Int J Cancer. 2008 Jul 15;123(2):340-7. PMID:18398842
      B16‐BL6細胞（メラノーマ細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • New highly potent and specific E6 and E7 siRNAs for treatment of HPV16 positive cervical cancer.
      Yamato K, Yamada T, Kizaki M, Ui-Tei K, Natori Y, Fujino M, Nishihara T, Ikeda Y, Nasu Y, Saigo K, Yoshinouchi M.
      Cancer Gene Ther. 2008 Mar;15(3):140-53. PMID:18157144
      SiHa細胞（子宮頸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.
      Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R.
      Cancer Sci. 2008 Apr;99(4):810-5. PMID:18377429
      HeLa細胞（子宮頸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Nek2 as an effective target for inhibition of tumorigenic growth and peritoneal dissemination of cholangiocarcinoma.
      Kokuryo T, Senga T, Yokoyama Y, Nagino M, Nimura Y, Hamaguchi M.
      Cancer Res. 2007 Oct 15;67(20):9637-42. PMID:17942892
      HuCCT1細胞（胆管がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。また、腹膜播種モデルマウスへの腹腔内投与。
    • Single-injection ornithine decarboxylase-directed antisense therapy using atelocollagen to suppress human cancer growth.
      Nakazawa K, Nemoto T, Hata T, Seyama Y, Nagahara S, Sano A, Itoh H, Nagai Y, Kubota S.
      Cancer. 2007 Mar 1;109(5):993-1002. PMID:17318877
      MKN45細胞（胃がん細胞）、COLO201細胞（大腸がん細胞）、RD細胞（横紋筋肉腫細胞）の皮下腫瘍モデルへのアンチセンスオリゴ局所投与。また、同モデルへのアンチセンスオリゴ腹腔内投与および筋肉内投与。
    • Tumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cells.
      Tazawa H, Tsuchiya N, Izumiya M, Nakagama H.
      Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15472-7. PMID:17875987
      HCT116細胞およびRKO細胞（大腸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Involvement of aminopeptidase N in enhanced chemosensitivity to paclitaxel in ovarian carcinoma in vitro and in vivo.
      Yamashita M, Kajiyama H, Terauchi M, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F.
      Int J Cancer. 2007 May 15;120(10):2243-50. PMID:17266036
      ES‐2細胞（卵巣がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Intratumor injection of small interfering RNA-targeting human papillomavirus 18 E6 and E7 successfully inhibits the growth of cervical cancer.
      Fujii T, Saito M, Iwasaki E, Ochiya T, Takei Y, Hayashi S, Ono A, Hirao N, Nakamura M, Kubushiro K, Tsukazaki K, Aoki D.
      Int J Oncol. 2006 Sep;29(3):541-8. PMID:16865269
      SKG-II細胞（子宮頸がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Small interfering RNA targeting epidermal growth factor receptor enhances chemosensitivity to cisplatin, 5-fluorouracil and docetaxel in head and neck squamous cell carcinoma.
      Nozawa H, Tadakuma T, Ono T, Sato M, Hiroi S, Masumoto K, Sato Y.
      Cancer Sci. 2006 Oct;97(10):1115-24. PMID:16984384
      SAS細胞（口腔扁平上皮がん細胞）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • Combinational antitumor effect of siRNA against midkine and paclitaxel on growth of human prostate cancer xenografts.
      Takei Y, Kadomatsu K, Goto T, Muramatsu T.
      Cancer. 2006 Aug 15;107(4):864-73. PMID:16832814
      PC‐3細胞（前立腺がん細胞の方）の皮下腫瘍モデルマウスへのsiRNA局所投与。
    • The human papillomavirus E6 and E7 inducible oncogene, hWAPL, exhibits potential as a therapeutic target.
      Kuroda M, Kiyono T, Oikawa K, Yoshida K, Mukai K.
      Br J Cancer. 2005 Jan 31;92(2):290-3. PMID:15655544
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    • Photomechanical Wave-Driven Delivery of siRNAs Targeting Intermediate Filament Proteins Promotes Functional Recovery after Spinal Cord Injury in Rats
      Takahiro Ando, Shunichi Sato mail, Terushige Toyooka, Hiroaki Kobayashi, Hiroshi Nawashiro, Hiroshi Ashida, Minoru Obara.
      PLoS One. 2012;7(12):e51744. PMID: 20181596
      脊髄損傷モデルラットの脊髄損傷部へのsiRNA髄腔内投与。
    • Down-regulation of glial fibrillary acidic protein and vimentin by RNA interference improves acute urinary dysfunction associated with spinal cord injury in rats.
      Toyooka T, Nawashiro H, Shinomiya N, Shima K.
      J Neurotrauma. 2011 Apr;28(4):607-18. PMID:21250919
      脊髄損傷モデルラットの脊髄損傷部表面へのsiRNA局所投与。
    • An siRNA against JC virus (JCV) agnoprotein inhibits JCV infection in JCV-producing cells inoculated in nude mice.
      Matoba T, Orba Y, Suzuki T, Makino Y, Shichinohe H, Kuroda S, Ochiya T, Itoh H, Tanaka S, Nagashima K, Sawa H.
      Neuropathology. 2008 Jun;28(3):286-94. PMID:18179406
      進行性多巣性白質脳症モデルマウスの線条体へのsiRNAマイクロインジェクション。
    • Transient receptor potential ankyrin 1 channels in the bladder mediate low temperature elicited bladder overactivity in rats.
      Imamura T, Ogawa T, Minagawa T, Daimon H, Nagai T, Ueno M, Saito T, Ishizuka O.
      Neurourol Urodyn. 2024 Jan;43(1):276-288. PMID: 38010891.
      過活動膀胱モデルラットの膀胱へのsiRNA経カテーテル投与。
    • The Role of microRNAs Related to Apoptosis for N-Methyl-d-Aspartic Acid-Induced Neuronal Cell Death in the Murine Retina.
      Sone K, Mori A, Sakamoto K, Nakahara T.
      Int J Mol Sci. 2024 Jan 16;25(2):1106. PMID: 38256177.
      （緑内障の研究）マウス硝子体内へのmiRNAおよびmiRNA inhibitor局所投与。
    • Tie1 contributes to the development of ovarian hyperstimulation syndrome under the regulation of EGR1 in granulosa cells.
      Sun L, Tian H, Xue S, Ye H, Xue X, Wang R, Liu Y, Zhang C, Chen Q, Gao S.
      Exp Mol Med. 2022 Jan;54(1):81-90. PMID: 35079118.
      卵巣過剰刺激症候群モデルラットの卵管の付け根へのsiRNAマイクロインジェクション。
    • The matrix vesicle cargo miR-125b accumulates in the bone matrix, inhibiting bone resorption in mice.
      Minamizaki T, Nakao Y, Irie Y, Ahmed F, Itoh S, Sarmin N, Yoshioka H, Nobukiyo A, Fujimoto C, Niida S, Sotomaru Y, Tanimoto K, Kozai K, Sugiyama T, Bonnelye E, Takei Y, Yoshiko Y.
      Commun Biol. 2020 Jan 16;3(1):30. PMID: 31949279
      LPS誘導性頭蓋骨溶解モデルマウスのLPS投与部周辺へのmiRNA局所投与。
    • Suppression of NLRP3 inflammasome improves alveolar bone defect healing in diabetic rats.
      Li H, Zhong X, Chen Z, Li W.
      J Orthop Surg Res. 2019 May 30;14(1):167. PMID: 31146750
      糖尿病モデルラットの歯槽骨欠損部へのshRNA局所投与。
    • Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein β degradation by adenosine receptor A1.
      Mitani T, Watanabe S, Yoshioka Y, Katayama S, Nakamura S, Ashida H.
      Biochim Biophys Acta. 2017 Sep 28;1864(12):2438-2448. PMID: 28965824
      （肥満の研究）マウスの精巣上体周辺脂肪組織へのsiRNA局所投与。
    • PACAP suppresses dry eye signs by stimulating tear secretion.
      Nakamachi T, Ohtaki H, Seki T, Yofu S, Kagami N, Hashimoto H, Shintani N, Baba A, Mark L, Lanekoff I, Kiss P, Farkas J, Reglodi D, Shioda S.
      Nat Commun. 2016 Jun 27;7:12034. PMID: 27345595
      ドライアイモデルマウスの眼窩涙腺周囲へのsiRNA局所投与。
    • Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells.
      Chelakkot-Govindalayathil AL, Mifuji-Moroka R, D'Alessandro-Gabazza CN, Toda M, Matsuda Y, Gil-Bernabe P, Roeen Z, Yasuma T, Yano Y, Gabazza EC, Iwasa M, Takei Y.
      J Thromb Haemost. 2015 Jan;13(1):142-54. PMID: 25399514
      アルコール性肝炎モデルマウスへのsiRNA腹腔内投与。
    • Attenuated AMH signaling pathway plays an important role in the pathogenesis of ovarian hyperstimulation syndrome.
      Wang L, Li H, Ai J, Yue J, Li Z, Zhang H, Zhao Y.
      Am J Transl Res. 2015 Oct 15;7(10):1925-38.
      卵巣過剰刺激症候群モデルマウスの卵嚢へのsiRNA局所投与。
    • A critical role for thymic stromal lymphopoietin in nickel-induced allergy in mice.
      Ashrin MN, Arakaki R, Yamada A, Kondo T, Kurosawa M, Kudo Y, Watanabe M, Ichikawa T, Hayashi Y, Ishimaru N.
      J Immunol. 2014 May 1;192(9):4025-31. PMID: 24670797
      金属アレルギーモデルマウスの耳介へのsiRNA局所投与。
    • Osteopontin knockdown in the kidneys of hyperoxaluric rats leads to reduction in renal calcium oxalate crystal deposition.
      Tsuji H, Shimizu N, Nozawa M, Umekawa T, Yoshimura K, De Velasco MA, Uemura H, Khan SR.
      Urolithiasis. 2014 Jun;42(3):195-202. PMID: 24619192
      腎結石モデルラットの腎被膜下および腎皮質内へのsiRNA局所投与。
    • Regulation of mucosal mast cell activation by short interfering RNAs targeting syntaxin4.
      Liu S, Nugroho AE, Shudou M, Maeyama K.
      Immunol Cell Biol. 2012 Mar;90(3):337-45. PMID:21625250
      喘息モデルラットの気管へのsiRNA経カテーテル投与。
    • Targeted suppression of HO-2 gene expression impairs the innate anti-inflammatory and repair responses of the cornea to injury.
      Bellner L, Patil KA, Castellano K, Halilovic A, Dunn MW, Schwartzman ML.
      Mol Vis. 2011 Apr 29;17:1144-52. PMID:21552471
      角膜傷害モデルマウスの結膜下へのshRNA発現プラスミド局所投与。また、同プラスミドの点眼投与。
    • A novel DC therapy with manipulation of MKK6 gene on nickel allergy in mice.
      Watanabe M, Ishimaru N, Ashrin MN, Arakaki R, Yamada A, Ichikawa T, Hayashi Y.
      PLoS One. 2011 Apr 22;6(4):e19017. PMID:21544193
      金属アレルギーモデルマウスの耳介へのsiRNA局所投与。
    • Targeted conversion of the transthyretin gene in vitro and in vivo.
      Nakamura M, Ando Y, Nagahara S, Sano A, Ochiya T, Maeda S, Kawaji T, Ogawa M, Hirata A, Terazaki H, Haraoka K, Tanihara H, Ueda M, Uchino M, Yamamura K.
      Gene Ther. 2004 May;11(10):838-46. PMID:14961068
      家族性アミロイドポリニューロパチーモデルマウスの肝臓への一本鎖オリゴヌクレオチド局所投与。
    • Biomaterials for gene delivery: atelocollagen-mediated controlled release of molecular medicines.
      Ochiya T, Nagahara S, Sano A, Itoh H, Terada M.
      Curr Gene Ther. 2001 May;1(1):31-52. PMID:12109137
      （徐放性および繰り返し投与の研究）腹腔内へのアデノウイルスベクター局所投与。