AteloCell コラーゲンスポンジ ハニカム | 株式会社高研

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  • CSH-96

  • CSH-10

コラーゲンスポンジ ハニカム
Atelocollagen, Honeycomb Sponge

概要

コラーゲンスポンジ ハニカムはアテロコラーゲンを独自の方法で加工、凍結乾燥したスポンジでポア(孔)が蜂の巣状に一定方向に密に並んだ構造をしています。高密度培養用担体として、また再生医療の研究に必要な三次元培養用のスキャフォールドとして活用できます。

用途

  • 三次元培養

  • 細胞移植

  • 生理活性物質の除放

特長

  • ハニカム構造により栄養供給および老廃物の放出が容易に行なわれ、ポアの内面全体に細胞を増殖させることができます。
  • コラゲナーゼ処理によって容易に細胞を回収できます。

コラーゲンの種類

ウシ皮由来アテロコラーゲン

保管方法:常温

  • CSH-96の実体顕微鏡画像

  • CSH-10の実体顕微鏡画像

  • ハニカムの電子顕微鏡画像

  • 線維芽細胞培養後のハニカム
    の電子顕微鏡画像

製品番号 製品 包装 価格(税別)
CSH-10 コラーゲンスポンジ ハニカム 無菌
Atelocollagen, Honeycomb Sponge
ハニカムスポンジ1個の大きさ : 約3 × 3 × 2mm
表面積:約2,000cm²/g
単位体積当りの重量:12〜13mg/mL
ポアサイズ 200〜400μm 
100mg/本 ¥ 25,000
CSH-96 ハニカム ディスク 96 無菌
Atelocollagen, Honeycomb Disc 96
ハニカムディスク1個の大きさ : 約φ6 × 2mm
ポアサイズ 200〜400μm
25枚入り/本 ¥ 26,000

本品は研究用試薬です。

参考文献

  • 神経・血管・筋肉
  • 骨・軟骨
  • その他
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      MCP-1およびsSiglec-9をハニカムに添加し、ラット顔面神経切断モデルへ移植。
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    • Correlation between antizyme 1 and differentiation of vascular smooth muscle cells cultured in honeycomb-like type-I collagen matrix.
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      Amino Acids. 2012 Feb;42(2-3):565-75. PMID:21894530
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    • Degradation of filamin induces contraction of vascular smooth muscle cells in type-I collagen matrix honeycombs.
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      Cell Physiol Biochem. 2011;27(6):669-80. PMID:21691085
      血管平滑筋細胞をハニカムに播種し、三次元培養。
    • Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs.
      Suzuki T, Ishii I, Kotani A, Masuda M, Hirata K, Ueda M, Ogata T, Sakai T, Ariyoshi N, Kitada M.
      Microvasc Res. 2009 Mar;77(2):143-149. PMID: 18848952
      血管平滑筋細胞をハニカムに播種し、三次元培養。
    • The axonal regeneration across a honeycomb collagen sponge applied to the transected spinal cord.
      Fukushima K, Enomoto M, Tomizawa S, Takahashi M, Wakabayashi Y, Itoh S, Kuboki Y, Shinomiya K.
      J Med Dent Sci. 2008 Mar;55(1):71-79. PMID:19845152
      後根神経節(DRG)をハニカム上で培養。また、ハニカムを脊髄損傷部へ移植。
    • Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells.
      Saeki K, Yogiashi Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo Y, Yuo A.
      J Cell Physiol. 2008 Oct;217(1):261-80. PMID: 18551514
      ES細胞由来血管内皮細胞をハニカムに播種し、マウス腹腔内へ移植。
    • Histological and functional analysis of vascular smooth muscle cells in a novel culture system with honeycomb-like structure.
      Ishii I, Tomizawa A, Kawachi H, Suzuki T, Kotani A, Koshushi I, Itoh H, Morisaki N, Bujo H, Saito Y, Ohmori S, Kitada M.
      Atherosclerosis. 2001 Oct;158(2):377-84. PMID: 11583716
      血管平滑筋細胞をハニカムに播種し、三次元培養。
    • Mechanical properties and structure-function relationships of human chondrocyte-seeded cartilage constructs after in vitro culture.
      Middendorf, J. M., Griffin, D. J., Shortkroff, S., Dugopolski, C., Kennedy, S., Siemiatkoski, J., Cohen, I. and Bonassar, L. J.
      J Orthop Res. 2017 Oct;35(10):2298-2306 PMID: 28169453
      軟骨細胞をハニカムに播種し、三次元培養。
    • In Vitro Culture Increases Mechanical Stability of Human Tissue Engineered Cartilage Constructs by Prevention of Microscale Scaffold Buckling.
      Jill M. Middendorf, Sonya Shortkroff, Caroline Dugopolski, Stephen Kennedy, Joseph Siemiatkoski, Lena R. Bartell, Itai Cohen, Lawrence J. Bonassar.
      J Biomech. 2017 Nov 7;64:77-84. PMID: 28964498
      軟骨細胞をハニカムに播種し、三次元培養。
    • A Novel Regenerative Technique Combining Bone Morphogenetic Protein-2 With Fibroblast Growth Factor-2 for Circumferential Defects in Dog Incisors.
      Saito E, Saito A, Kato H, Shibukawa Y, Inoue S, Yuge F, Nakajima T, Takahashi T, Kawanami M.
      J Periodontol. 2016 Sep;87(9):1067-74. PMID: 27240475
      BMP-2およびFGF-2をハニカムに添加し、イヌ水平性歯周組織欠損部へ移植。
    • Utility of T2 mapping and dGEMRIC for evaluation of cartilage repair after allograft chondrocyte implantation in a rabbit model.
      Endo J, Watanabe A, Sasho T, Yamaguchi S, Saito M, Akagi R, Muramatsu Y, Mukoyama S, Katsuragi J, Akatsu Y, Fukawa T, Okubo T, Osone F, Takahashi K.
      Osteoarthritis Cartilage. 2015 Feb;23(2):280-8. PMID:25450842
      軟骨細胞をハニカムに播種し、ウサギ骨軟骨欠損部へ移植。
    • Inflammatory response of intervertebral disc cells is reduced by fibrin sealant scaffold in vitro.
      Buser Z, Liu J, Thorne KJ, Coughlin D, Lotz JC.
      J Tissue Eng Regen Med. 2014 Jan;8(1):77-84. PMID:22610998
      線維輪由来細胞をハニカムへ播種し、三次元培養。
    • Application of dedifferentiated fat cells for periodontal tissue regeneration.
      Sugawara A, Sato S.
      Hum Cell. 2014 Jan;27(1):12-21. PMID: 24068600
      脱分化脂肪細胞をハニカムに播種し骨分化誘導後、ラット歯槽骨欠損部へ移植。
    • Carbon nanotubes functionalized with fibroblast growth factor accelerate proliferation of bone marrow-derived stromal cells and bone formation.
      Hirata E, Ménard-Moyon C, Venturelli E, Takita H, Watari F, Bianco A, Yokoyama A.
      Nanotechnology. 2013 Nov 1;24(43):435101. PMID: 24077482
      骨髄由来間質細胞をFGF+カーボンナノチューブでコーティングしたハニカムに播種し、ラット頭頂骨骨膜下へ移植。
    • Experimental Formation of Dentin-like Structure in the Root Canal Implant Model Using Cryopreserved Swine Dental Pulp Progenitor Cells
      Kostas Kodonas, Christos Gogos, Serafim Papadimitriou, Kokona Kouzi-Koliakou, Dimitrios Tziafas
      J Endod. 2012 Jul;38(7):913-9. PMID: 22703653
      歯髄幹細胞をハニカムに播種し、ミニブタ抜歯窩へ移植。
    • Human telomerase reverse transcriptase and glucose-regulated protein 78 increase the life span of articular chondrocytes and their repair potential.
      Sato M, Shin-Ya K, Lee JI, Ishihara M, Nagai T, Kaneshiro N, Mitani G, Tahara H, Mochida J.
      BMC Musculoskelet Disord. 2012 Apr 2;13(1):51. PMID:22472071
      軟骨細胞をコラーゲン膜つきハニカム特注品に播種し、ウサギ骨軟骨欠損部へ移植。
    • Multiwalled carbon nanotube-coating of 3D collagen scaffolds for bone tissue engineering.
      Hirata E, Uo M, Takita H, Akasaka T, Watari F, Yokoyama A.
      Carbon 2011;149(10):3284-3291.
      カーボンナノチューブでハニカムをコーティングし、ラット大腿骨欠損部へ移植。
    • Calcified Honeycomb-shaped Collagen Maintains its Geometry in Vivo and Effectively Induces Vasculature and Osteogenesis.
      Yoshinori KUBOKI, Shouhei IKU, Ryota YOSHIMOTO, Tohru KAKU, Hiroko TAKITA, Dong LI, Yasuo KOKAI, Shunji YUNOKI, Rachel L. SAMMONS, Kazuhide OZEKI, and Teruo MIYATA.
      Nano Biomed. 2009;1(2): 85-94.
      HApでハニカムをコーティングし、ラット皮下へ移植。
    • Bone tissue reaction of nano-hydroxyapatite/collagen composite at the early stage of implantation.
      Fukui N, Sato T, Kuboki Y, Aoki H.
      Biomed Mater Eng. 2008;18(1):25-33. PMID: 18198404
      n-HApでハニカムをコーティングし、ウサギ下顎骨欠損部へ移植。
    • Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold.
      Kakudo N, Shimotsuma A, Miyake S, Kushida S, Kusumoto K.
      J Biomed Mater Res A. 2008 Jan;84(1):191-197. PMID:17607760
      脂肪組織由来間葉系幹細胞をハニカムに播種し、マウスに背部皮下に移植。
    • Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study.
      Takeuchi R, Ryo A, Komitsu N, Mikuni-Takagaki Y, Fukui A, Takagi Y, Shiraishi T, Morishita S, Yamazaki Y, Kumagai K, Aoki I, Saito T.
      Arthritis Res Ther. 2008;10(4):R77. PMID: 18616830
      中足趾節関節由来軟骨細胞をハニカムに播種し、低出力超音波パルスを与えながら培養。
    • Markedly different effects of hyaluronic acid and chondroitin sulfate-A on the differentiation of human articular chondrocytes in micromass and 3-D honeycomb rotation cultures.
      Banu N, Tsuchiya T.
      J Biomed Mater Res A. 2007 Feb;80(2):257-67. PMID: 16941596
      軟骨細胞をハニカムに播種し、回転培養。
    • Mechanism of bone induction by KUSA/A1 cells using atelocollagen honeycomb scaffold.
      Hidetsugu T, Paola RA, Hitoshi N, Mehmet G, Jin LY, Borkosky SS, Liliana M, Noriyuki N.
      J Biomed Sci. 2007 Mar;14(2):255-263. PMID: 17061146
      間葉系幹細胞をハニカムに播種し、マウス背部に移植。
    • Effects of growth factors on heparin-carrying polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering.
      Sato M, Ishihara M, Ishihara M, Kaneshiro N, Mitani G, Nagai T, Kutsuna T, Asazuma T, Kikuchi M, Mochida J.
      J Biomed Mater Res B Appl Biomater. 2007 Oct;83(1):181-8. PMID: 17385220
      軟骨細胞をコラーゲン膜付ハニカム特注品に播種し、三次元培養。
    • Differentiation of mesenchymal stem cells into osteoblasts on honeycomb collagen scaffolds.
      George J, Kuboki Y, Miyata T.
      Biotechnol Bioeng. 2006 Oct 20;95(3):404-11. PMID: 16572435
      間葉系幹細胞をハニカムに播種し、三次元培養。
    • Tissue engineering of articular cartilage with autologous cultured adipose tissue-derived stromal cells using atelocollagen honeycomb-shaped scaffold with a membrane sealing in rabbits.
      Masuoka K, Asazuma T, Hattori H, Yoshihara Y, Sato M, Matsumura K, Matsui T, Takase B, Nemoto K, Ishihara M.
      J Biomed Mater Res B Appl Biomater. 2006 Oct;79(1):25-34. PMID: 16506181
      脂肪由来間質細胞をコラーゲン膜付ハニカム特注品に播種し、ウサギ軟骨欠損部へ移植。
    • Effects of vibration and hyaluronic acid on activation of three-dimensional cultured chondrocytes.
      Takeuchi R, Saito T, Ishikawa H, Takigami H, Dezawa M, Ide C, Itokazu Y, Ikeda M, Shiraishi T, Morishita S.
      Arthritis Rheum. 2006 Jun;54(6):1897-905. PMID:16736525
      軟骨細胞をハニカムに播種し、三次元培養。
    • Efficacy of atelocollagen honeycomb scaffold in bone formation using KUSA/A1 cells. 
      Rodriguez AP, Missana L, Nagatsuka H, Gunduz M, Tsujigiwa H, Rivera R, Nagai N.
      J Biomed Mater Res A. 2006 Jun 15;77(4):707-717. PMID:16555268
      間葉系幹細胞をハニカムに播種し、マウス皮下へ移植。
    • An atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS-scaffold) for the culture of annulus fibrosus cells from an intervertebral disc.
      Sato M, Asazuma T, Ishihara M, Kikuchi T, Masuoka K, Ichimura S, Kikuchi M, Kurita A, Fujikawa K.
      J Biomed Mater Res A. 2003 Feb 1;64(2):248-56. PMID:12522811
      線維輪細胞をコラーゲン膜付きハニカム特注品に播種し、三次元培養。
    • Tissue engineering of the intervertebral disc with cultured annulus fibrosus cells using atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS scaffold).
      Sato M, Kikuchi M, Ishihara M, Ishihara M, Asazuma T, Kikuchi T, Masuoka K, Hattori H, Fujikawa K.
      Med Biol Eng Comput. 2003 May;41(3):365-71. PMID: 12803304
      線維輪細胞をコラーゲン膜付ハニカム特注品に播種し、ウサギ椎間板空隙へ移植。
    • Development of a cranium-infection rat model for artificial bone implantation.
      Inoue Y, Sakamoto Y, Ochiai H, Yoshimura Y, Okumoto T.
      Biomed Res Clin Prac, 2016 Volume 1(1): 18-21.
      黄色ブドウ球菌をハニカムに播種し、ラット頭蓋骨欠損部へ移植。
    • A three-dimensional collagen-sponge-based culture system coated with simplified recombinant fibronectin improves the function of a hepatocyte cell line.
      Nishida Y, Taniguchi A.
      In Vitro Cell Dev Biol Anim. 2016 Mar;52(3):271-7. PMID: 26714750
      フィブロネクチンのコラーゲンゲン結合ドメインと細胞結合ドメインからなる融合タンパク質でハニカムをコーティングし、肝細胞を培養。
    • Cinnamtannin B-1 Promotes Migration of Mesenchymal Stem Cells and Accelerates Wound Healing in Mice.
      Fujita K, Kuge K, Ozawa N, Sahara S, Zaiki K, Nakaoji K, Hamada K, Takenaka Y, Tanahashi T, Tamai K, Kaneda Y, Maeda A.
      PLoS One. 2015 Dec 11;10(12):e0144166. PMID: 26657737
      Cinnamtannin B-1をハニカムに添加し、マウス創傷部へ移植。
    • Red blood cell generation by three-dimensional aggregate cultivation of late erythroblasts.
      Lee E, Han SY, Choi HS, Chun B, Hwang B, Baek EJ.
      Tissue Eng Part A. 2015 Feb;21(3-4):817-28. PMID: 25314917
      赤芽球をハニカムに播種し、三次元培養。
    • Mallotus philippinensis bark extracts promote preferential migration of mesenchymal stem cells and improve wound healing in mice.
      Furumoto T, Ozawa N, Inami Y, Toyoshima M, Fujita K, Zaiki K, Sahara S, Akita M, Kitamura K, Nakaoji K, Hamada K, Tamai K, Kaneda Y, Maeda A.
      Phytomedicine. 2014 Feb 15;21(3):247-53. PMID: 24182990
      クスノガシワ抽出物をハニカムに添加し、マウス創傷部へ移植。
    • 3D-fection: cell transfection within 3D scaffolds and hydrogels.
      Sapet C, Formosa C, Sicard F, Bertosio E, Zelphati O, Laurent N.
      Ther Deliv. 2013 Jun;4(6):673-85. PMID: 23738666
      脂肪由来間質細胞、オリゴデンドロサイト、胎児腎細胞、子宮頸がん細胞、血管内皮細胞、間葉系幹細胞、軟骨細胞、腎臓細胞、線維芽細胞、神経幹細胞、マクロファージをハニカムにそれぞれ播種し、三次元培養。
    • 3D collagen scaffolds coated with multiwalled carbon nanotubes: initial cell attachment to internal surface.
      Hirata E, Uo M, Nodasaka Y, Takita H, Ushijima N, Akasaka T, Watari F, Yokoyama A.
      J Biomed Mater Res B Appl Biomater. 2010 May;93(2):544-550. PMID: 20186828
      カーボンナノチューブでハニカムをコーティングし、骨肉腫細胞を培養。
    • ES細胞と間葉系細胞とのモザイクスフィアからのin vitro毛髪新生。(In vitro and de novo generation of hair from mosaic spheres formed jointly from ES and mesenchymal cells.)
      八巻真理子
      日本再生医療学会雑誌 2009 8(2):91-97.
      ES細胞と間葉系幹細胞をハニカムに播種し、マウス背筋膜下に移植。
    • I型コラーゲンの人工細胞外マトリックスはマウス胚性幹細胞の腫瘍化能を抑制する。(Artificial extracellular matrix of type I collagen can suppress the tumorigenetic potential of mouse embryonic stem cells.)
      八巻真理子
      日本再生医療学会雑誌 2009 8(1):109-114.
      ES細胞(胚様体)をハニカムに播種し、マウス腎被膜下に移植。
    • Biodegradable honeycomb collagen scaffold for dermal tissue engineering.
      George J, Onodera J, Miyata T.
      J Biomed Mater Res A. 2008 Dec 15;87(4):1103-11. PMID: 18792951
      線維芽細胞をハニカムに播種し、三次元培養。
    • An experimental study of novel bioartificial materials applied to glycotechnology for tissue engineering.
      Megumi Hayashi, Yukio Sumi, Hirokazu Mizuno, Hideki Mizutani, Minoru Ueda, Ken-ichiro Hata.
      Materials Science and Engineering: C. Volume 24, Issue 3, 1 April 2004, Pages 447–455.
      ラクトースやマンニトール、アセチルグルコサミンをハニカムに結合させ、マウス皮下へ移植。
    • Embryonic stem cell-derived embryoid bodies in three-dimensional culture system form hepatocyte-like cells in vitro and in vivo.
      Imamura T, Cui L, Teng R, Johkura K, Okouchi Y, Asanuma K, Ogiwara N, Sasaki K.
      Tissue Eng. 2004 Nov-Dec;10(11-12):1716-24. PMID: 15684680
      ES細胞(胚様体)をハニカムに播種し、部分肝切除マウスモデルの肝中間葉へ移植。
    • A honeycomb collagen carrier for cell culture as a tissue engineering scaffold.
      Itoh H, Aso Y, Furuse M, Noishiki Y, Miyata T.
      Artif Organs. 2001 Mar;25(3):213-7. PMID: 11284889
      線維芽細胞、血管内皮細胞、卵巣細胞、腎細胞をハニカムに播種し、三次元培養。
    • Development of composite cultured oral mucosa utilizing collagen sponge matrix and contracted collagen gel: a preliminary study for clinical applications.
      Moriyama T, Asahina I, Ishii M, Oda M, Ishii Y, Enomoto S.
      Tissue Eng. 2001 Aug;7(4):415-27. PMID: 11506731
      線維芽細胞含有コラーゲン溶液をハニカムに添加してゲル化。さらに角化細胞を播種し、マウス創傷部へ移植。
    • Arg-gingipain a DNA vaccine induces protective immunity against infection by Porphyromonas gingivalis in a murine model.
      Yonezawa H, Ishihara K, Okuda K.
      Infect Immun. 2001 May;69(5):2858-64. PMID:11292699
      rgpA DNAワクチンをマウスに接種。マウス血清をハニカムに添加し、Porphyromonas gingivalisの接着を評価。