Three-step three-dimensional culture for the experiment you don’t want to ruin

－Perfect for a small remaining budget－

As the end of the fiscal year approaches, many researchers have to report their findings within a limited timeframe and budget. Although eager to actively proceed with their research experiments, some hesitate to try three-dimensional (3D) culture due to inconsistent results among experimenters.

In such cases, 3D Ready Atelocollagen, introduced in this newsletter, will be helpful.

The above images show 3D cultures of GFP-expressing HepG2 cells. The gel on the right contains a low concentration of atelocollagen, and the cells have formed a sediment at the bottom (based on in-house data).

Why does this happen and how can it be avoided? To gelate commonly available atelocollagen acidic solutions for 3D culture, the solution must be neutralized by mixing buffer with the culture medium in advance. There is a concern that the results may vary depending on who performs this preparation step, particularly in laboratories accommodating many researchers. 3D Ready Atelocollagen resolves this issue.

3D Ready Atelocollagen was developed to make stable 3D cultures accessible to both beginners and experts. Because the product is a ready-to-use, neutral pH solution of atelocollagen premixed with DMEM , it reduces variability in experimental outcomes between researchers.