Inhibition of bladder cancer growth using siRNA and miRNA

Article information
Regulation of SLD5 gene expression by miR-370 during acute growth of cancer cells.
Yamane K, Naito H, Wakabayashi T, Yoshida H, Muramatsu F, Iba T, Kidoya H, Takakura N
Sci Rep. 2016 Aug 8;6:30941. PMID: 27499248

Overview
The GINS complex, which is involved in the regulation of DNA polymerase ε, is constituted from the SLD 5 and PSF 1-3 proteins, although only PSF 1-3 have been implicated in cancer progression. Given that there are no reports on the expression level of SLD5 and the progress of cancer, research was performed to clarify the role of SLD5.

First, a comparison between human bladder cancer cells and normal cells showed that expression of SLD5 is enhanced in cancer cells.

Next, when SLD5 siRNA was administered in a bladder cancer xenograft model using AteloGene, the expression level of SLD5 was reduced to about a half that of a control group, and tumour growth was markedly suppressed.

Subsequently, the authors searched for miRNAs that negatively regulate SLD5 expression. This analysis identified miR-370 as a negative regulator of SLD5, while also showing that miR-370 expression was decreased in human bladder cancer cells.

Furthermore, administration of miR-370 using AteloGene (to the same model as used for the siRNA experiments) revealed a significant inhibitory effect on the expression of SLD5 and on tumour growth compared to the control group.

Improvement of AteloGene Local Use yields increased gelation speed at the site of administration and enhanced transfection efficiency

Product name
 – AteloGene Local Use “Quick Gelation” (Cat. No.: #1492 for Cosmo Bio customers）
                                                                  (Cat. No.: #1494 for REPROCELL customers)