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Seeds for Regenerative Medicine in 5 and 10 Years
Clinical Applications Based on Basic Research
－Atelocollagen for research use from a medical device manufacturer－
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According to the website of the Alliance for Regenerative Medicine, 78 regenerative medicine products were approved worldwide as of March 2024. Excluding products specifically related to cancer immunotherapy or gene therapy, 18 products are classified as Tissue Engineering Products, of which six contain collagen.
Atelocollagen has been used as a medical device in clinics for over 30 years, and is currently being used in basic research on clinical applications in regenerative medicine, among others. In this newsletter we present three techniques for cell culturing used in regenerative medicine research, along with excerpts from papers where our atelocollagen products were used for each.
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Three Experimental Techniques for Regenerative Medicine Research and Product Use Cases for Each Technique:
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① Large-volume cell cultures of mesenchymal stem cells (MSCs) using microcarriers
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100% atelocollagen microspheres that can be dissolved using collagenase during cell recovery.
Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
Mashiko T, Kanayama K, Saito N, Shirado T, Asahi R, Mori M, Yoshimura K.
Cells. 2021 Mar 4;10(3):560. PMID: 33806638
In this paper, adipose-derived MSCs were seeded onto microspheres and then cultured under dynamic or static conditions.
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② Production of iPS cell-derived skin models using a collagen gel
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A high-purity atelocollagen solution made from the same raw materials as our atelocollagen medical devices.
Restoration of keratinocytic phenotypes in autonomous trisomy-rescued cells.
Tanuma-Takahashi A, Inoue M, Kajiwara K, Takagi R, Yamaguchi A, Samura O, Akutsu H, Sago H, Kiyono T, Okamoto A, Umezawa A. Stem Cell Res Ther. 2021 Aug 25;12(1):476. PMID: 34433490
In this paper, a skin model was created by seeding iPS cell-derived keratinocytes onto a fibroblast-containing collagen gel.
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③ Production of multi-layered vascular sheets using a permeable collagen membrane
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A highly transparent, atelocollagen-based substance-permeable membrane that is suitable for transplantation.
Hydrostatic pressure under hypoxia facilitates fabrication of tissue-engineered vascular grafts derived from human vascular smooth muscle cells in vitro.
Kojima T, Nakamura T, Saito J, Hidaka Y, Akimoto T, Inoue H, Chick CN, Usuki T, Kaneko M, Miyagi E, Ishikawa Y, Yokoyama U. Acta Biomater. 2023 Nov;171:209-222. PMID: 37793599.
In this paper, vascular smooth muscle cells were first seeded onto a collagen membrane and exposed to hydrostatic pressure under hypoxia. Multi-layered cell sheets were fabricated by repeating this process.
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④ Transplantation of liver organoids using a collagen gel
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An atelocollagen solution with low antigenicity is applicable for transplantation.
Syngeneic implantation of mouse hepatic progenitor cell-derived three-dimensional liver tissue with dense collagen fibrils.
Tamai M, Adachi E, Kawase M, Tagawa YI.
World J Gastroenterol. 2022 Apr 14;28(14):1444-1454. PMID: 35582675.
In this paper, liver organoids consisting of hepatic progenitor cells and mouse embryonic fibroblasts were generated in a collagen gel before being transplanted into a hepatectomy mouse model.
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⑤ Transplantation of iPS cell-derived neural crest cells using a collagen sponge
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An atelocollagen sponge featuring a honeycomb-like pore structure with all pores uniformly aligned.
Human iPSC-derived neural crest stem cells can produce EPO and induce erythropoiesis in anemic mice.
Brizi V, Buttò S, Cerullo D, Michele Lavecchia A, Rodrigues-Diez R, Novelli R, Corna D, Benigni A, Remuzzi G, Xinaris C.
Stem Cell Res. 2021 Aug;55:102476. PMID: 34339993
In this paper, iPS cell-derived neural crest cells were seeded onto honeycomb discs and then transplanted subcutaneously into mice.
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⑥ Transplantation of dental pulp stem cells using a collagen sponge
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An atelocollagen sponge with a random pore structure and strength comparable to that of cartilage.
Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model.
Rikitake K, Kunimatsu R, Yoshimi Y, Nakajima K, Hiraki T, Aisyah Rizky Putranti N, Tsuka Y, Abe T, Ando K, Hayashi Y, Nikawa H, Tanimoto K.
Oral Dis. 2023 Mar;29(2):725-734. PMID: 34510661.
In this paper, dental pulp stem cells from deciduous teeth were seeded onto MIGHTY, an atelocollagen sponge, and then were transplanted into mouse skull defects.
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We offer various other types of atelocollagen scaffolds, so please click on the button below for additional details.
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We plan to participate in two exhibitions in March 2024. Please visit us at the venue to see our various atelocollagen products in person.
1. The 23rd Congress of the Japanese Society for Regenerative Medicine
Dates ：March 21 (Wednesday) to March 23 (Saturday) 2024
Venue：Toki Messe
Booth ：B10
2. The 144th Annual Meeting of the Pharmaceutical Society of Japan
Dates ：March 29 (Friday) to March 31 (Sunday) 2024
Venue：Pacifico Yokohama
Booth ：61
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