Newsletters
Why not try in vitro experiments under conditions better resembling those in the body, instead of the usual in vitro experiments?
03 August 2015
In this edition of the newsletter, we introduce an article in which cancer cells and fibroblasts were co-cultured using Atelcollagen Acidic Solution I-PC 5 mg/mL.
Observation of cancer cell invasion under in vitro conditions resembling those in the living body
Article information
Oncogenic Ras/ERK signaling activates CDCP1 to promote tumor invasion and metastasis.
Uekita T, Fujii S, Miyazawa Y, Iwakawa R, Narisawa-Saito M, Nakashima K, Tsuta K, Tsuda H, Kiyono T, Yokota J, Sakai R.
Mol Cancer Res. 2014 Oct;12(10):1449-59. PMID: 24939643
Overview
In this paper, collagen gel was generated using Atelocollagen Acidic Solution and fibroblasts, and human cervical cancer cells were seeded on the collagen gel to create an environment resembling that in the living body.
In addition, frozen sections of the gel were prepared after 4 weeks of culture, and infiltration by the cancer cells was evaluated following fixation and staining.
For researchers interested in co-culture or 3D culture but concerned that now is not the right time, consider this cost-effective and highly versatile collagen acidic solution.
Product name
– Atelocollagen Acidic Solution 3 mg/mL (Cat. No.: IPC-30)
– Atelocollagen Acidic Solution 5 mg/mL (Cat. No.: IPC-50)
