Method for systemic administration of AteloGene | KOKEN CO., LTD. | KOKEN CO., LTD.

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Method for systemic administration

The standard single dose for a mouse is 200 μL of AteloGene® & siRNA/miRNA mixture. The upper limit of the one-time dose is 200 μL. Be sure not to exceed this dose. Repeated administrations, e.g. once a week for 3 weeks, twice a week for 2 weeks, etc., are also applicable.

  1. Restrain the mouse, if necessary.
  2. Wipe the tail of the mouse to disinfect it andto increase the visibility of the vein.
  3. Insert the needle into the tail vein at a position approximately 1/4 to 1/3 of the tail length from the tail end.
  4. Confirm that the needle has entered the vessel and then slowly inject 200 μL of the mixture.
    The injection speed should be less than 10 μL/s

Evaluation of the siRNA/miRNA transfection effect

Effects of siRNA/miRNA transfections with AteloGene® may differ depending on the siRNA/miRNA sequences, expression levels of target genes, target tissues, etc. Please consider optimizing siRNA/miRNA concentrations, administration frequencies and timings for administrations and evaluations accordingly.