Show usage menuUsage
Method for systemic administration
The standard single dose for a mouse is 200 μL of AteloGene® & siRNA/miRNA mixture. The upper limit of the one-time dose is 200 μL. Be sure not to exceed this dose. Repeated administrations, e.g. once a week for 3 weeks, twice a week for 2 weeks, etc., are also applicable.
- Restrain the mouse, if necessary.
- Wipe the tail of the mouse to disinfect it andto increase the visibility of the vein.
- Insert the needle into the tail vein at a position approximately 1/4 to 1/3 of the tail length from the tail end.
- Confirm that the needle has entered the vessel and then slowly inject 200 μL of the mixture.
The injection speed should be less than 10 μL/s
Evaluation of the siRNA/miRNA transfection effect
Effects of siRNA/miRNA transfections with AteloGene® may differ depending on the siRNA/miRNA sequences, expression levels of target genes, target tissues, etc. Please consider optimizing siRNA/miRNA concentrations, administration frequencies and timings for administrations and evaluations accordingly.